Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (<em>Cosmopolites sordidus</em>)
Fungal Volatile Organic Compounds (VOCs) repel banana black weevil (BW), <i>Cosmopolites sordidus</i> (Germar, 1824), the key-pest of banana [<i>Musa</i> sp. (Linnaeus, 1753)]. The entomopathogens <i>Beauveria bassiana</i> (Bb1TS11) and <i>Metarhizium robert...
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2020-08-01
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| author | Ana Lozano-Soria Ugo Picciotti Federico Lopez-Moya Javier Lopez-Cepero Francesco Porcelli Luis Vicente Lopez-Llorca |
| author_facet | Ana Lozano-Soria Ugo Picciotti Federico Lopez-Moya Javier Lopez-Cepero Francesco Porcelli Luis Vicente Lopez-Llorca |
| author_sort | Ana Lozano-Soria |
| collection | DOAJ |
| description | Fungal Volatile Organic Compounds (VOCs) repel banana black weevil (BW), <i>Cosmopolites sordidus</i> (Germar, 1824), the key-pest of banana [<i>Musa</i> sp. (Linnaeus, 1753)]. The entomopathogens <i>Beauveria bassiana</i> (Bb1TS11) and <i>Metarhizium robertsii</i> (Mr4TS04) were isolated from banana plantation soils using an insect bait. Bb1TS11 and Mr4TS04 were pathogenic to BW adults. Bb1TS11, Bb203 (from infected palm weevils), Mr4TS04 and the nematophagous fungus <i>Pochonia clamydosporia</i> (Pc123), were tested for VOCs production. VOCs were identified by Gas Chromatography/Mass Spectrometry–Solid-Phase Micro Extraction (GC/MS-SPME). GC/MS-SPME identified a total of 97 VOCs in all strains tested. Seven VOCs (styrene, benzothiazole, camphor, borneol, 1,3-dimethoxy-benzene, 1-octen-3-ol and 3-cyclohepten-1-one) were selected for their abundance or previous record as insect repellents. In olfactometry bioassays, BW-starved adults in the dark showed the highest mobility to banana corm. 2-cyclohepten-1-one (C7), commercially available isomer of 3-cyclohepten-1-one, is the best BW repellent (<i>p</i> < 0.05), followed by 1,3-dimethoxy-benzene (C5). The rest of the VOCs have a milder repellency to BW. Styrene (C1) and benzothiazole (C2) (known to repel palm weevil) block the attraction of banana corm and BW pheromone to BW adults in bioassays. Therefore, VOCs from biocontrol fungi can be used in future studies to biomanage BW in the field. |
| format | Article |
| id | doaj-art-9b61dcee64fb43169736e72d60327774 |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2020-08-01 |
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| series | Insects |
| spelling | doaj-art-9b61dcee64fb43169736e72d603277742025-08-20T03:30:51ZengMDPI AGInsects2075-44502020-08-0111850910.3390/insects11080509Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (<em>Cosmopolites sordidus</em>)Ana Lozano-Soria0Ugo Picciotti1Federico Lopez-Moya2Javier Lopez-Cepero3Francesco Porcelli4Luis Vicente Lopez-Llorca5Department of Marine Science and Applied Biology, Laboratory of Plant Pathology, University of Alicante, 03080 Alicante, SpainDepartment of Marine Science and Applied Biology, Laboratory of Plant Pathology, University of Alicante, 03080 Alicante, SpainDepartment of Marine Science and Applied Biology, Laboratory of Plant Pathology, University of Alicante, 03080 Alicante, SpainTechnical Department of Coplaca, 38001 Tenerife, SpainDepartment of Soil, Plant, and Food Sciences—(DiSSPA), University of Bari Aldo Moro, 70126 Bari, ItalyDepartment of Marine Science and Applied Biology, Laboratory of Plant Pathology, University of Alicante, 03080 Alicante, SpainFungal Volatile Organic Compounds (VOCs) repel banana black weevil (BW), <i>Cosmopolites sordidus</i> (Germar, 1824), the key-pest of banana [<i>Musa</i> sp. (Linnaeus, 1753)]. The entomopathogens <i>Beauveria bassiana</i> (Bb1TS11) and <i>Metarhizium robertsii</i> (Mr4TS04) were isolated from banana plantation soils using an insect bait. Bb1TS11 and Mr4TS04 were pathogenic to BW adults. Bb1TS11, Bb203 (from infected palm weevils), Mr4TS04 and the nematophagous fungus <i>Pochonia clamydosporia</i> (Pc123), were tested for VOCs production. VOCs were identified by Gas Chromatography/Mass Spectrometry–Solid-Phase Micro Extraction (GC/MS-SPME). GC/MS-SPME identified a total of 97 VOCs in all strains tested. Seven VOCs (styrene, benzothiazole, camphor, borneol, 1,3-dimethoxy-benzene, 1-octen-3-ol and 3-cyclohepten-1-one) were selected for their abundance or previous record as insect repellents. In olfactometry bioassays, BW-starved adults in the dark showed the highest mobility to banana corm. 2-cyclohepten-1-one (C7), commercially available isomer of 3-cyclohepten-1-one, is the best BW repellent (<i>p</i> < 0.05), followed by 1,3-dimethoxy-benzene (C5). The rest of the VOCs have a milder repellency to BW. Styrene (C1) and benzothiazole (C2) (known to repel palm weevil) block the attraction of banana corm and BW pheromone to BW adults in bioassays. Therefore, VOCs from biocontrol fungi can be used in future studies to biomanage BW in the field.https://www.mdpi.com/2075-4450/11/8/509Curculionidae<i>Musa accuminata</i>invasive speciespest controlbiological controlVOCs |
| spellingShingle | Ana Lozano-Soria Ugo Picciotti Federico Lopez-Moya Javier Lopez-Cepero Francesco Porcelli Luis Vicente Lopez-Llorca Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (<em>Cosmopolites sordidus</em>) Insects Curculionidae <i>Musa accuminata</i> invasive species pest control biological control VOCs |
| title | Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (<em>Cosmopolites sordidus</em>) |
| title_full | Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (<em>Cosmopolites sordidus</em>) |
| title_fullStr | Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (<em>Cosmopolites sordidus</em>) |
| title_full_unstemmed | Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (<em>Cosmopolites sordidus</em>) |
| title_short | Volatile Organic Compounds from Entomopathogenic and Nematophagous Fungi, Repel Banana Black Weevil (<em>Cosmopolites sordidus</em>) |
| title_sort | volatile organic compounds from entomopathogenic and nematophagous fungi repel banana black weevil em cosmopolites sordidus em |
| topic | Curculionidae <i>Musa accuminata</i> invasive species pest control biological control VOCs |
| url | https://www.mdpi.com/2075-4450/11/8/509 |
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