Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2
A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aim...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/457380 |
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| author | Eudes Euler de Souza Lucena Fausto Pierdoná Guzen José Rodolfo Lopes de Paiva Cavalcanti Maria Jocileide de Medeiros Marinho Wogelsanger Oliveira Pereira Carlos Augusto Galvão Barboza Miriam Stela Mariz de Oliveira Costa Expedito Silva do Nascimento Júnior Jeferson Sousa Cavalcante |
| author_facet | Eudes Euler de Souza Lucena Fausto Pierdoná Guzen José Rodolfo Lopes de Paiva Cavalcanti Maria Jocileide de Medeiros Marinho Wogelsanger Oliveira Pereira Carlos Augusto Galvão Barboza Miriam Stela Mariz de Oliveira Costa Expedito Silva do Nascimento Júnior Jeferson Sousa Cavalcante |
| author_sort | Eudes Euler de Souza Lucena |
| collection | DOAJ |
| description | A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4. Cells cultivated only with conditioned medium acquired a glial phenotype. Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs. |
| format | Article |
| id | doaj-art-9b59e1087c1943e3915dd65aee195dff |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-9b59e1087c1943e3915dd65aee195dff2025-02-03T01:03:07ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/457380457380Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2Eudes Euler de Souza Lucena0Fausto Pierdoná Guzen1José Rodolfo Lopes de Paiva Cavalcanti2Maria Jocileide de Medeiros Marinho3Wogelsanger Oliveira Pereira4Carlos Augusto Galvão Barboza5Miriam Stela Mariz de Oliveira Costa6Expedito Silva do Nascimento Júnior7Jeferson Sousa Cavalcante8Laboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, BrazilLaboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, BrazilLaboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, BrazilLaboratory of Molecular Biology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, BrazilLaboratory of Molecular Biology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, BrazilDepartment of Morphology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, BrazilLaboratory of Neuroanatomy, Department of Morphology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, BrazilLaboratory of Neuroanatomy, Department of Morphology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, BrazilLaboratory of Neurochemical Studies, Department of Physiology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, BrazilA number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4. Cells cultivated only with conditioned medium acquired a glial phenotype. Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs.http://dx.doi.org/10.1155/2014/457380 |
| spellingShingle | Eudes Euler de Souza Lucena Fausto Pierdoná Guzen José Rodolfo Lopes de Paiva Cavalcanti Maria Jocileide de Medeiros Marinho Wogelsanger Oliveira Pereira Carlos Augusto Galvão Barboza Miriam Stela Mariz de Oliveira Costa Expedito Silva do Nascimento Júnior Jeferson Sousa Cavalcante Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2 The Scientific World Journal |
| title | Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2 |
| title_full | Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2 |
| title_fullStr | Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2 |
| title_full_unstemmed | Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2 |
| title_short | Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2 |
| title_sort | plasticity of mesenchymal stem cells from mouse bone marrow in the presence of conditioned medium of the facial nerve and fibroblast growth factor 2 |
| url | http://dx.doi.org/10.1155/2014/457380 |
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