Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Abstract The purpose of chronic hepatitis B (CHB) treatment is functional cure (FC). It refers to the removal of hepatitis B surface antigen (HBsAg) from the serum. However, this outcome is rare with current treatments due to the presence of integrated hepatitis B virus (HBV) DNA and intrahepatic co...
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BMC
2025-07-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-025-04062-z |
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| author | Meng Wang Qi Zhou Huan Wu Yefu Wang |
| author_facet | Meng Wang Qi Zhou Huan Wu Yefu Wang |
| author_sort | Meng Wang |
| collection | DOAJ |
| description | Abstract The purpose of chronic hepatitis B (CHB) treatment is functional cure (FC). It refers to the removal of hepatitis B surface antigen (HBsAg) from the serum. However, this outcome is rare with current treatments due to the presence of integrated hepatitis B virus (HBV) DNA and intrahepatic covalently closed circular DNA (cccDNA). This study investigates the efficacy of removal of HBsAg using immunoadsorption. An immunosorbent was prepared by coupling anti-HBsAg monoclonal antibodies to agarose beads, selected from six candidates based on adsorption efficiency. Then the adsorbents were characterized by scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS). V79 lung fibroblasts were used to determine the cytotoxicity of the immunosorbent through in vitro viability assays. The results indicated that the immunosorbent was non-cytotoxic. Static and dynamic adsorption studies in vitro demonstrated efficient HBsAg removal. To validate therapeutic potential, an extracorporeal circulation system equipped with the immunosorbent (10 ml sorbent column) was tested in rabbits injected with HBsAg-positive rabbit serum. This system selectively reduced serum HBsAg levels by 76.38 ± 1.37% and HBV DNA by 66.20 ± 0.85%. Given the absence of existing therapies capable of reducing HBsAg directly, our findings suggest that combining immunoadsorption with HBV drugs may serve as a novel therapeutic strategy—or at least provide a valuable adjunct—to advance functional cure efforts in CHB. Further clinical validation is warranted. |
| format | Article |
| id | doaj-art-9b56353cd7d743f2ab72e24a515b1cc8 |
| institution | Kabale University |
| issn | 1471-230X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj-art-9b56353cd7d743f2ab72e24a515b1cc82025-08-20T04:01:35ZengBMCBMC Gastroenterology1471-230X2025-07-0125111110.1186/s12876-025-04062-zEfficacy of extracorporeal immunoadsorption therapy for removal of HBsAgMeng Wang0Qi Zhou1Huan Wu2Yefu Wang3State Key Laboratory of Virology, College of Life Sciences, Wuhan UniversityState Key Laboratory of Virology, College of Life Sciences, Wuhan UniversityState Key Laboratory of Virology, College of Life Sciences, Wuhan UniversityState Key Laboratory of Virology, College of Life Sciences, Wuhan UniversityAbstract The purpose of chronic hepatitis B (CHB) treatment is functional cure (FC). It refers to the removal of hepatitis B surface antigen (HBsAg) from the serum. However, this outcome is rare with current treatments due to the presence of integrated hepatitis B virus (HBV) DNA and intrahepatic covalently closed circular DNA (cccDNA). This study investigates the efficacy of removal of HBsAg using immunoadsorption. An immunosorbent was prepared by coupling anti-HBsAg monoclonal antibodies to agarose beads, selected from six candidates based on adsorption efficiency. Then the adsorbents were characterized by scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS). V79 lung fibroblasts were used to determine the cytotoxicity of the immunosorbent through in vitro viability assays. The results indicated that the immunosorbent was non-cytotoxic. Static and dynamic adsorption studies in vitro demonstrated efficient HBsAg removal. To validate therapeutic potential, an extracorporeal circulation system equipped with the immunosorbent (10 ml sorbent column) was tested in rabbits injected with HBsAg-positive rabbit serum. This system selectively reduced serum HBsAg levels by 76.38 ± 1.37% and HBV DNA by 66.20 ± 0.85%. Given the absence of existing therapies capable of reducing HBsAg directly, our findings suggest that combining immunoadsorption with HBV drugs may serve as a novel therapeutic strategy—or at least provide a valuable adjunct—to advance functional cure efforts in CHB. Further clinical validation is warranted.https://doi.org/10.1186/s12876-025-04062-zHBVHBsAgMonoclonal antibodyAgarose beadsExtracorporeal immunoadsorptionRabbits |
| spellingShingle | Meng Wang Qi Zhou Huan Wu Yefu Wang Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg BMC Gastroenterology HBV HBsAg Monoclonal antibody Agarose beads Extracorporeal immunoadsorption Rabbits |
| title | Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg |
| title_full | Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg |
| title_fullStr | Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg |
| title_full_unstemmed | Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg |
| title_short | Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg |
| title_sort | efficacy of extracorporeal immunoadsorption therapy for removal of hbsag |
| topic | HBV HBsAg Monoclonal antibody Agarose beads Extracorporeal immunoadsorption Rabbits |
| url | https://doi.org/10.1186/s12876-025-04062-z |
| work_keys_str_mv | AT mengwang efficacyofextracorporealimmunoadsorptiontherapyforremovalofhbsag AT qizhou efficacyofextracorporealimmunoadsorptiontherapyforremovalofhbsag AT huanwu efficacyofextracorporealimmunoadsorptiontherapyforremovalofhbsag AT yefuwang efficacyofextracorporealimmunoadsorptiontherapyforremovalofhbsag |