Microbiological study of fish from the rural market of Katana on the edge of Kahuzi-Biega National Park, South Kivu Province, DRC
Introduction Safeguarding public health requires effective planning and adequate epidemiological surveillance. Microbial foodborne illnesses are a major public health problem. Purpose This study aims to assess bacterial infestation in fish sold at the rural market of Katana, located on th...
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Orapuh, Inc.
2025-05-01
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| Online Access: | https://www.orapuh.org/ojs/ojs-3.1.2-4/index.php/orapj/article/view/380 |
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| author | Lushombo Bunani KAZADI MINZANGI Kabongo Kanimba Ololo Tshiamala Efoloko Bokungu Bahizire Kayeye Valery Byenda Badesire Zigashane olivier Mutabazi Muhindo |
| author_facet | Lushombo Bunani KAZADI MINZANGI Kabongo Kanimba Ololo Tshiamala Efoloko Bokungu Bahizire Kayeye Valery Byenda Badesire Zigashane olivier Mutabazi Muhindo |
| author_sort | Lushombo Bunani |
| collection | DOAJ |
| description |
Introduction
Safeguarding public health requires effective planning and adequate epidemiological surveillance. Microbial foodborne illnesses are a major public health problem.
Purpose
This study aims to assess bacterial infestation in fish sold at the rural market of Katana, located on the edge of the Kahuzi-Biega National Park in South Kivu Province, Democratic Republic of the Congo.
Methods
Fish samples were collected, and bacterial cultures were performed on culture media. Identification was conducted using Gram staining at the Bacteriology Laboratory of the Fomulac/Katana General Reference Hospital in Katana, located in Kabare Territory, north of Bukavu City. For morphological identification, each sample was analysed in triplicate. Data were statistically analysed using the F test and the χ² test with Past version 4.10 software.
Results
Rod-shaped bacteria accounted for 52.6% of isolates, of which 93.3% were Gram-negative and 6.6% were Gram-positive. Among the Gram-negative bacteria, 21.4% were identified as Vibrio cholerae, while 71.9% were classified as other bacteria. Gram-positive bacteria were observed in species of Haplochromis sp. and Limnotrissa miodon. Gram-negative diplococci comprised 95.4% of isolates, compared to 4.5% that were Gram-positive. Among cocci, Gram-positive cocci accounted for 60%, while Gram-negative cocci made up 40%. The Hikojima strain of Vibrio cholerae was detected in 100% of Vibrio cholerae colonies. Biochemical identification revealed the presence of Vibrio cholerae, Citrobacter sp., Enterobacter sp., Salmonella sp., Escherichia coli, and Klebsiella sp. Statistical analysis showed no significant difference between fish species and the bacteria sought or between fish species and fish condition. However, there was a significant difference between colony characteristics and both the culture medium and fish condition, while no significant difference was observed between the culture medium and the bacteria sought.
Conclusion
Fish sold at Katana's rural market are contaminated with bacteria regardless of the conditioning methods used. The unsanitary conditions of the market, display cases, and vendors' hygiene practices are the primary sources of contamination.
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| format | Article |
| id | doaj-art-9b53c2ca6e254beb896d0026a35a7298 |
| institution | OA Journals |
| issn | 2644-3740 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Orapuh, Inc. |
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| spelling | doaj-art-9b53c2ca6e254beb896d0026a35a72982025-08-20T02:30:35ZengOrapuh, Inc.Orapuh Journal2644-37402025-05-016410.4314/orapj.v6i4.40Microbiological study of fish from the rural market of Katana on the edge of Kahuzi-Biega National Park, South Kivu Province, DRCLushombo Bunani0KAZADI MINZANGI1Kabongo Kanimba2Ololo Tshiamala3Efoloko Bokungu4Bahizire Kayeye5Valery Byenda Badesire6Zigashane olivier7Mutabazi Muhindo8Higher Pedagogical Institute of Kabare, ISP Kabare, South Kivu, Democratic Republic of the CongoUniversity of Kinshasa, Kinshasa, Democratic Republic of the CongoResearch Centre for Applied and Technological Sciences, CRSAT/Kinshasa, Democratic Republic of the CongoResearch Centre for Applied and Technological Sciences, CRSAT/Kinshasa, Democratic Republic of the CongoResearch Centre for Applied and Technological Sciences, CRSAT/Kinshasa, Democratic Republic of the CongoHigher Pedagogical Institute of Kabare, ISP Kabare, South Kivu, Democratic Republic of the CongoHigher Pedagogical Institute of Kabare, ISP Kabare, South Kivu, Democratic Republic of the CongoHigher Pedagogical Institute of Kabare, ISP Kabare, South Kivu, Democratic Republic of the CongoHigher Pedagogical Institute of Kabare, ISP Kabare, South Kivu, Democratic Republic of the Congo Introduction Safeguarding public health requires effective planning and adequate epidemiological surveillance. Microbial foodborne illnesses are a major public health problem. Purpose This study aims to assess bacterial infestation in fish sold at the rural market of Katana, located on the edge of the Kahuzi-Biega National Park in South Kivu Province, Democratic Republic of the Congo. Methods Fish samples were collected, and bacterial cultures were performed on culture media. Identification was conducted using Gram staining at the Bacteriology Laboratory of the Fomulac/Katana General Reference Hospital in Katana, located in Kabare Territory, north of Bukavu City. For morphological identification, each sample was analysed in triplicate. Data were statistically analysed using the F test and the χ² test with Past version 4.10 software. Results Rod-shaped bacteria accounted for 52.6% of isolates, of which 93.3% were Gram-negative and 6.6% were Gram-positive. Among the Gram-negative bacteria, 21.4% were identified as Vibrio cholerae, while 71.9% were classified as other bacteria. Gram-positive bacteria were observed in species of Haplochromis sp. and Limnotrissa miodon. Gram-negative diplococci comprised 95.4% of isolates, compared to 4.5% that were Gram-positive. Among cocci, Gram-positive cocci accounted for 60%, while Gram-negative cocci made up 40%. The Hikojima strain of Vibrio cholerae was detected in 100% of Vibrio cholerae colonies. Biochemical identification revealed the presence of Vibrio cholerae, Citrobacter sp., Enterobacter sp., Salmonella sp., Escherichia coli, and Klebsiella sp. Statistical analysis showed no significant difference between fish species and the bacteria sought or between fish species and fish condition. However, there was a significant difference between colony characteristics and both the culture medium and fish condition, while no significant difference was observed between the culture medium and the bacteria sought. Conclusion Fish sold at Katana's rural market are contaminated with bacteria regardless of the conditioning methods used. The unsanitary conditions of the market, display cases, and vendors' hygiene practices are the primary sources of contamination. https://www.orapuh.org/ojs/ojs-3.1.2-4/index.php/orapj/article/view/380Food poisoningFish infestationVibrio cholerae coloniesBiochemical identification of bacteriaGram-negative diplococci |
| spellingShingle | Lushombo Bunani KAZADI MINZANGI Kabongo Kanimba Ololo Tshiamala Efoloko Bokungu Bahizire Kayeye Valery Byenda Badesire Zigashane olivier Mutabazi Muhindo Microbiological study of fish from the rural market of Katana on the edge of Kahuzi-Biega National Park, South Kivu Province, DRC Orapuh Journal Food poisoning Fish infestation Vibrio cholerae colonies Biochemical identification of bacteria Gram-negative diplococci |
| title | Microbiological study of fish from the rural market of Katana on the edge of Kahuzi-Biega National Park, South Kivu Province, DRC |
| title_full | Microbiological study of fish from the rural market of Katana on the edge of Kahuzi-Biega National Park, South Kivu Province, DRC |
| title_fullStr | Microbiological study of fish from the rural market of Katana on the edge of Kahuzi-Biega National Park, South Kivu Province, DRC |
| title_full_unstemmed | Microbiological study of fish from the rural market of Katana on the edge of Kahuzi-Biega National Park, South Kivu Province, DRC |
| title_short | Microbiological study of fish from the rural market of Katana on the edge of Kahuzi-Biega National Park, South Kivu Province, DRC |
| title_sort | microbiological study of fish from the rural market of katana on the edge of kahuzi biega national park south kivu province drc |
| topic | Food poisoning Fish infestation Vibrio cholerae colonies Biochemical identification of bacteria Gram-negative diplococci |
| url | https://www.orapuh.org/ojs/ojs-3.1.2-4/index.php/orapj/article/view/380 |
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