Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays
Objective. To estimate performance characteristics and diagnostic value of immunofluorescent islet cell antibody (ICA) assay, immunoradiometric glutamic acid decarboxylase antibody (GADA) assay, and ELISA tyrosine phosphatase IA-2 antibody (IA-2A) and insulin antibody (IA) assays.Research Design and...
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Endocrinology Research Centre
2016-11-01
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| Series: | Сахарный диабет |
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| Online Access: | https://www.dia-endojournals.ru/jour/article/view/8032 |
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| author | Alexei V. Timofeev Ksenia A. Gorst Valentin Y. Uvarov Ekaterina A. Pronina Alisa V. Vitebskaya Anastasiya V. Popovich Anatoliy N. Tiulpakov Natalia A. Zubkova Olesya A. Gioeva Yulia V. Tikhonovich Alexander V. Ilin Oleg Y. Latyshev Goar F. Okminjan Ksenia L. Kabolova Julianna A. Kanokova Leokadia Y. Zhuleva Igor E. Koltunov Elena E. Petriaikina Irina G. Rybkina Irina V. Garyaeva Salome L. Guguchia Angelina B. Shimarova Artiom V. Bullikh Irina G. Kolomina Evgenia A. Evsyukova Sergei S. Bukin Natalia N. Egarmina Natalia Y. Arbatskaya Elina Y. Yanovskaya Vladimir I. Popenko Alexander A. Arkhipkin |
| author_facet | Alexei V. Timofeev Ksenia A. Gorst Valentin Y. Uvarov Ekaterina A. Pronina Alisa V. Vitebskaya Anastasiya V. Popovich Anatoliy N. Tiulpakov Natalia A. Zubkova Olesya A. Gioeva Yulia V. Tikhonovich Alexander V. Ilin Oleg Y. Latyshev Goar F. Okminjan Ksenia L. Kabolova Julianna A. Kanokova Leokadia Y. Zhuleva Igor E. Koltunov Elena E. Petriaikina Irina G. Rybkina Irina V. Garyaeva Salome L. Guguchia Angelina B. Shimarova Artiom V. Bullikh Irina G. Kolomina Evgenia A. Evsyukova Sergei S. Bukin Natalia N. Egarmina Natalia Y. Arbatskaya Elina Y. Yanovskaya Vladimir I. Popenko Alexander A. Arkhipkin |
| author_sort | Alexei V. Timofeev |
| collection | DOAJ |
| description | Objective. To estimate performance characteristics and diagnostic value of immunofluorescent islet cell antibody (ICA) assay, immunoradiometric glutamic acid decarboxylase antibody (GADA) assay, and ELISA tyrosine phosphatase IA-2 antibody (IA-2A) and insulin antibody (IA) assays.Research Design and Methods. Antibodies were tested in 438 children and adolescents with newly diagnosed diabetes mellitus (DM) type 1, and in 891 subjects without DM type 1. ICA were determined by the classic indirect immunofluorescent method recommended by the Juvenile Diabetes Foundation International, GADA were determined with the Immunotech IRMA Anti-GAD kit, and IA-2A and IA were determined with Medizym Anti-IA2 and Orgentec Anti-Insulin ELISA kits, respectively. Sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV) of the tests were estimated with contingency tables. Diagnostic accuracy was estimated from areas under receiver operating curves (AUC).Results. ICA test was of the greatest diagnostic value (Se=88%, Sp=96%, PPV=96%, NPV=94%, AUC=0,94), followed by IA-2A (Se=66%, Sp=98%, PPV=98%, NPV=59%, AUC=0,82) and GADA (Se=73%, Sp=84%, PPV=75%, NPV=83%, AUC=0,79). IA test exhibited a very low Se (4,3%) and lacked diagnostic accuracy (AUC=0,5).Conclusions. We recommend to use ICA, IA-2A and GADA tests surveyed in our study for diagnosis of DM type 1 and differential diagnosis of DM. We don’t recommend IA testing with an Orgentec Anti-Insulin ELISA kit for usage in clinical practice. |
| format | Article |
| id | doaj-art-9b477f127dc84b3cbb0b91b1699fd658 |
| institution | DOAJ |
| issn | 2072-0351 2072-0378 |
| language | English |
| publishDate | 2016-11-01 |
| publisher | Endocrinology Research Centre |
| record_format | Article |
| series | Сахарный диабет |
| spelling | doaj-art-9b477f127dc84b3cbb0b91b1699fd6582025-08-20T03:09:03ZengEndocrinology Research CentreСахарный диабет2072-03512072-03782016-11-0119433134010.14341/DM80327512Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assaysAlexei V. Timofeev0Ksenia A. Gorst1Valentin Y. Uvarov2Ekaterina A. Pronina3Alisa V. Vitebskaya4Anastasiya V. Popovich5Anatoliy N. Tiulpakov6Natalia A. Zubkova7Olesya A. Gioeva8Yulia V. Tikhonovich9Alexander V. Ilin10Oleg Y. Latyshev11Goar F. Okminjan12Ksenia L. Kabolova13Julianna A. Kanokova14Leokadia Y. Zhuleva15Igor E. Koltunov16Elena E. Petriaikina17Irina G. Rybkina18Irina V. Garyaeva19Salome L. Guguchia20Angelina B. Shimarova21Artiom V. Bullikh22Irina G. Kolomina23Evgenia A. Evsyukova24Sergei S. Bukin25Natalia N. Egarmina26Natalia Y. Arbatskaya27Elina Y. Yanovskaya28Vladimir I. Popenko29Alexander A. Arkhipkin30I.M.Sechenov First Moscow State Medical UniversityI.M.Sechenov First Moscow State Medical UniversityI.M.Sechenov First Moscow State Medical UniversityI.M.Sechenov First Moscow State Medical UniversityI.M.Sechenov First Moscow State Medical UniversityI.M.Sechenov First Moscow State Medical UniversityEndocrinology Research CentreEndocrinology Research CentreEndocrinology Research CentreEndocrinology Research CentreEndocrinology Research CentreRussian Medical Academy of Postgraduate EducationRussian Medical Academy of Postgraduate EducationRussian Medical Academy of Postgraduate EducationRussian Medical Academy of Postgraduate EducationRussian Medical Academy of Postgraduate EducationMorozov Children Clinical HospitalMorozov Children Clinical HospitalMorozov Children Clinical HospitalMorozov Children Clinical HospitalMorozov Children Clinical HospitalMorozov Children Clinical HospitalMorozov Children Clinical HospitalBashliaeva Children Clinical HospitalBashliaeva Children Clinical HospitalBashliaeva Children Clinical HospitalBashliaeva Children Clinical HospitalPirogov Russian National Research Medical UniversityPirogov Russian National Research Medical UniversityEngelhardt Institute of Molecular Biology Russian Academy of SciencesScientific Center EFIS Ltd.Objective. To estimate performance characteristics and diagnostic value of immunofluorescent islet cell antibody (ICA) assay, immunoradiometric glutamic acid decarboxylase antibody (GADA) assay, and ELISA tyrosine phosphatase IA-2 antibody (IA-2A) and insulin antibody (IA) assays.Research Design and Methods. Antibodies were tested in 438 children and adolescents with newly diagnosed diabetes mellitus (DM) type 1, and in 891 subjects without DM type 1. ICA were determined by the classic indirect immunofluorescent method recommended by the Juvenile Diabetes Foundation International, GADA were determined with the Immunotech IRMA Anti-GAD kit, and IA-2A and IA were determined with Medizym Anti-IA2 and Orgentec Anti-Insulin ELISA kits, respectively. Sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (NPV) of the tests were estimated with contingency tables. Diagnostic accuracy was estimated from areas under receiver operating curves (AUC).Results. ICA test was of the greatest diagnostic value (Se=88%, Sp=96%, PPV=96%, NPV=94%, AUC=0,94), followed by IA-2A (Se=66%, Sp=98%, PPV=98%, NPV=59%, AUC=0,82) and GADA (Se=73%, Sp=84%, PPV=75%, NPV=83%, AUC=0,79). IA test exhibited a very low Se (4,3%) and lacked diagnostic accuracy (AUC=0,5).Conclusions. We recommend to use ICA, IA-2A and GADA tests surveyed in our study for diagnosis of DM type 1 and differential diagnosis of DM. We don’t recommend IA testing with an Orgentec Anti-Insulin ELISA kit for usage in clinical practice.https://www.dia-endojournals.ru/jour/article/view/8032diabetes mellitusdiabetes mellitus type 1differential diagnosisautoantibodiesislet cell antibodyglutamic acid decarboxylase antibodytyrosine phosphatase antibodyinsulin antibodyimmunofluorescence assayradioimmune assayimmunoenzyme assa |
| spellingShingle | Alexei V. Timofeev Ksenia A. Gorst Valentin Y. Uvarov Ekaterina A. Pronina Alisa V. Vitebskaya Anastasiya V. Popovich Anatoliy N. Tiulpakov Natalia A. Zubkova Olesya A. Gioeva Yulia V. Tikhonovich Alexander V. Ilin Oleg Y. Latyshev Goar F. Okminjan Ksenia L. Kabolova Julianna A. Kanokova Leokadia Y. Zhuleva Igor E. Koltunov Elena E. Petriaikina Irina G. Rybkina Irina V. Garyaeva Salome L. Guguchia Angelina B. Shimarova Artiom V. Bullikh Irina G. Kolomina Evgenia A. Evsyukova Sergei S. Bukin Natalia N. Egarmina Natalia Y. Arbatskaya Elina Y. Yanovskaya Vladimir I. Popenko Alexander A. Arkhipkin Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays Сахарный диабет diabetes mellitus diabetes mellitus type 1 differential diagnosis autoantibodies islet cell antibody glutamic acid decarboxylase antibody tyrosine phosphatase antibody insulin antibody immunofluorescence assay radioimmune assay immunoenzyme assa |
| title | Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_full | Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_fullStr | Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_full_unstemmed | Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_short | Diagnostic value of islet autoantibody assays practised in Russia. 1. Classic immunofluorescence islet cell antibody assay, immunoradiometric glutamic acid decarboxylase antibody assay, and ELISA tyrosine phosphatase antibody and insulin antibody assays |
| title_sort | diagnostic value of islet autoantibody assays practised in russia 1 classic immunofluorescence islet cell antibody assay immunoradiometric glutamic acid decarboxylase antibody assay and elisa tyrosine phosphatase antibody and insulin antibody assays |
| topic | diabetes mellitus diabetes mellitus type 1 differential diagnosis autoantibodies islet cell antibody glutamic acid decarboxylase antibody tyrosine phosphatase antibody insulin antibody immunofluorescence assay radioimmune assay immunoenzyme assa |
| url | https://www.dia-endojournals.ru/jour/article/view/8032 |
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