Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond Electrode
Electrochemical detection of glucose was achieved at a glucose oxidase (GOx)-cobalt phthalocyanine (CoPc)-modified boron-doped diamond (BDD) electrode without any additional electron mediator in the electrolyte solution. The surface of the hydrogen-terminated BDD thin film prepared by microwave plas...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/943957 |
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| author | Takeshi Kondo Masaru Horitani Makoto Yuasa |
| author_facet | Takeshi Kondo Masaru Horitani Makoto Yuasa |
| author_sort | Takeshi Kondo |
| collection | DOAJ |
| description | Electrochemical detection of glucose was achieved at a glucose oxidase (GOx)-cobalt phthalocyanine (CoPc)-modified boron-doped diamond (BDD) electrode without any additional electron mediator in the electrolyte solution. The surface of the hydrogen-terminated BDD thin film prepared by microwave plasma-assisted CVD was modified with 4-vinylpyridine (4VP) via photochemical modification. The 4VP-BDD was then immersed in a CoPc solution to obtain CoPc-BDD. A poly(p-phenylenediamine) (PPD) thin film containing GOx was coated on the CoPc-BDD electrode surface via electropolymerization. At the GOx/PPD-CoPc-BDD electrode, anodic current for glucose oxidation was observed with a sigmoidal voltammetric curve, indicating successful electron mediation of H2O2 generated as the result of glucose oxidation at GOx. The signal-to-background ratio for voltammetric current of glucose detection was larger at the GOx/PPD-CoPc-BDD electrode than at the GOx/PPD-modified platinum electrode due to the smaller background current of the modified BDD electrode. |
| format | Article |
| id | doaj-art-9b4599576d5541e1838d018aa9562c02 |
| institution | Kabale University |
| issn | 2090-3529 2090-3537 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Electrochemistry |
| spelling | doaj-art-9b4599576d5541e1838d018aa9562c022025-02-03T01:22:32ZengWileyInternational Journal of Electrochemistry2090-35292090-35372012-01-01201210.1155/2012/943957943957Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond ElectrodeTakeshi Kondo0Masaru Horitani1Makoto Yuasa2Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, JapanDepartment of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, JapanDepartment of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda 278-8510, JapanElectrochemical detection of glucose was achieved at a glucose oxidase (GOx)-cobalt phthalocyanine (CoPc)-modified boron-doped diamond (BDD) electrode without any additional electron mediator in the electrolyte solution. The surface of the hydrogen-terminated BDD thin film prepared by microwave plasma-assisted CVD was modified with 4-vinylpyridine (4VP) via photochemical modification. The 4VP-BDD was then immersed in a CoPc solution to obtain CoPc-BDD. A poly(p-phenylenediamine) (PPD) thin film containing GOx was coated on the CoPc-BDD electrode surface via electropolymerization. At the GOx/PPD-CoPc-BDD electrode, anodic current for glucose oxidation was observed with a sigmoidal voltammetric curve, indicating successful electron mediation of H2O2 generated as the result of glucose oxidation at GOx. The signal-to-background ratio for voltammetric current of glucose detection was larger at the GOx/PPD-CoPc-BDD electrode than at the GOx/PPD-modified platinum electrode due to the smaller background current of the modified BDD electrode.http://dx.doi.org/10.1155/2012/943957 |
| spellingShingle | Takeshi Kondo Masaru Horitani Makoto Yuasa Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond Electrode International Journal of Electrochemistry |
| title | Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond Electrode |
| title_full | Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond Electrode |
| title_fullStr | Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond Electrode |
| title_full_unstemmed | Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond Electrode |
| title_short | Sensitive Electrochemical Detection of Glucose at Glucose Oxidase-Cobalt Phthalocyanine-Modified Boron-Doped Diamond Electrode |
| title_sort | sensitive electrochemical detection of glucose at glucose oxidase cobalt phthalocyanine modified boron doped diamond electrode |
| url | http://dx.doi.org/10.1155/2012/943957 |
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