Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test Systems
The objective: to study the genetic determinants of rifampicin resistance of Mycobacterium tuberculosis by sequencing the RRDR rpoB gene.Subjects and Methods. The study included 651 M. tuberculosis isolates that were tested for drug suceptibility (DST) by genotypic methods (gDST) TB-TEST (BIOCHIP-IM...
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2023-07-01
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| Series: | Туберкулез и болезни лёгких |
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| Online Access: | https://www.tibl-journal.com/jour/article/view/1733 |
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| author | E. A. Mazurina T. V. Umpeleva L. A. Golubeva L. S. Lavrenchuk D. V. Vakhrusheva I. A. Vasilyeva |
| author_facet | E. A. Mazurina T. V. Umpeleva L. A. Golubeva L. S. Lavrenchuk D. V. Vakhrusheva I. A. Vasilyeva |
| author_sort | E. A. Mazurina |
| collection | DOAJ |
| description | The objective: to study the genetic determinants of rifampicin resistance of Mycobacterium tuberculosis by sequencing the RRDR rpoB gene.Subjects and Methods. The study included 651 M. tuberculosis isolates that were tested for drug suceptibility (DST) by genotypic methods (gDST) TB-TEST (BIOCHIP-IMB, Russia), and Amplitube-MDR-RV (NPK Sintol, Russia) and phenotypic methods (phDST): the absolute concentration method, BACTEC MGIT 960 system, and Sensititre Myco TB kit. Sanger RRDR sequencing of the rpoB gene was performed for 20 isolates with discrepancies in the results of phDST and gDST.Results. As a result of sequencing, two variants of mutations in the RRDR region of the rpoB gene were found, which were not detected by TB-TEST and Amblitub MDR-RV test systems, however were strongly associated with rifampicin resistance of Mycobacterium tuberculosis. The most common variant was the insertion of three nucleotides (TTC) encoding the amino acid phenylalanine at position 434 of the codon (1296-1300insTTC). |
| format | Article |
| id | doaj-art-9b254fe445ed46afaf3e4ded13552a4e |
| institution | OA Journals |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| publishDate | 2023-07-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj-art-9b254fe445ed46afaf3e4ded13552a4e2025-08-20T01:47:41ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062023-07-01101310.58838/2075-1230-2023-101-3-69-771728Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test SystemsE. A. Mazurina0T. V. Umpeleva1L. A. Golubeva2L. S. Lavrenchuk3D. V. Vakhrusheva4I. A. Vasilyeva5Ural Phthisiopulmonology Research Institute – a Branch of National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of HealthUral Phthisiopulmonology Research Institute – a Branch of National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of HealthUral Phthisiopulmonology Research Institute – a Branch of National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of HealthUral Phthisiopulmonology Research Institute – a Branch of National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of HealthUral Phthisiopulmonology Research Institute – a Branch of National Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of HealthNational Medical Research Center of Phthisiopulmonology and Infectious Diseases, Russian Ministry of HealthThe objective: to study the genetic determinants of rifampicin resistance of Mycobacterium tuberculosis by sequencing the RRDR rpoB gene.Subjects and Methods. The study included 651 M. tuberculosis isolates that were tested for drug suceptibility (DST) by genotypic methods (gDST) TB-TEST (BIOCHIP-IMB, Russia), and Amplitube-MDR-RV (NPK Sintol, Russia) and phenotypic methods (phDST): the absolute concentration method, BACTEC MGIT 960 system, and Sensititre Myco TB kit. Sanger RRDR sequencing of the rpoB gene was performed for 20 isolates with discrepancies in the results of phDST and gDST.Results. As a result of sequencing, two variants of mutations in the RRDR region of the rpoB gene were found, which were not detected by TB-TEST and Amblitub MDR-RV test systems, however were strongly associated with rifampicin resistance of Mycobacterium tuberculosis. The most common variant was the insertion of three nucleotides (TTC) encoding the amino acid phenylalanine at position 434 of the codon (1296-1300insTTC).https://www.tibl-journal.com/jour/article/view/1733rifampicinmycobacterium tuberculosis (mtb)isolategenemutation |
| spellingShingle | E. A. Mazurina T. V. Umpeleva L. A. Golubeva L. S. Lavrenchuk D. V. Vakhrusheva I. A. Vasilyeva Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test Systems Туберкулез и болезни лёгких rifampicin mycobacterium tuberculosis (mtb) isolate gene mutation |
| title | Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test Systems |
| title_full | Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test Systems |
| title_fullStr | Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test Systems |
| title_full_unstemmed | Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test Systems |
| title_short | Genetic Determinants of Rifampicin Resistance of Mycobacterium tuberculosis not Included in the Russian Molecular Genetic Test Systems |
| title_sort | genetic determinants of rifampicin resistance of mycobacterium tuberculosis not included in the russian molecular genetic test systems |
| topic | rifampicin mycobacterium tuberculosis (mtb) isolate gene mutation |
| url | https://www.tibl-journal.com/jour/article/view/1733 |
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