Subretinal microglia support donor photoreceptor survival in rd1 mice
Abstract Purpose To investigate the potential relationship between subretinal microglia and transplanted donor photoreceptors. Methods Photoreceptor precursors were transplanted into wild-type mice and rd1 mice by trans-scleral injection. Immunohistochemistry was employed to detect microglia and mac...
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| Format: | Article |
| Language: | English |
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BMC
2024-11-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-024-04052-0 |
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| author | Qinjia Ren Fang Lu Ruwa Hao Yingying Chen Chen Liang |
| author_facet | Qinjia Ren Fang Lu Ruwa Hao Yingying Chen Chen Liang |
| author_sort | Qinjia Ren |
| collection | DOAJ |
| description | Abstract Purpose To investigate the potential relationship between subretinal microglia and transplanted donor photoreceptors. Methods Photoreceptor precursors were transplanted into wild-type mice and rd1 mice by trans-scleral injection. Immunohistochemistry was employed to detect microglia and macrophages. PlX5622 feed was used to achieve microglia depletion and microglia repopulation. RNA-seq and qPCR were utilized to evaluate gene expression. Confocal microscopy was used to observe the interaction between microglia and donor photoreceptors. Results Donor photoreceptors survived in rd1 mice but not in wild-type mice after trans-scleral injection. The microglial cells closely interacted with donor cells. While donor cells failed to survive in rd1 mice after microglia depletion, they could survive following microglia repopulation. The RNA-seq analysis showed a pro-neurodevelopmental effect of sub-retinal microglia/RPE tissue in rd1 mice. Conclusions Subretinal microglia supported donor photoreceptor survival in rd1 mice. |
| format | Article |
| id | doaj-art-9b232ab1995a458993bce370532d819e |
| institution | OA Journals |
| issn | 1757-6512 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj-art-9b232ab1995a458993bce370532d819e2025-08-20T02:33:00ZengBMCStem Cell Research & Therapy1757-65122024-11-0115111010.1186/s13287-024-04052-0Subretinal microglia support donor photoreceptor survival in rd1 miceQinjia Ren0Fang Lu1Ruwa Hao2Yingying Chen3Chen Liang4Department of Ophthalmology, West China Hospital, Sichuan UniversityDepartment of Ophthalmology, West China Hospital, Sichuan UniversityDepartment of Ophthalmology, West China Hospital, Sichuan UniversityDepartment of Ophthalmology, West China Hospital, Sichuan UniversityDepartment of Ophthalmology, West China Hospital, Sichuan UniversityAbstract Purpose To investigate the potential relationship between subretinal microglia and transplanted donor photoreceptors. Methods Photoreceptor precursors were transplanted into wild-type mice and rd1 mice by trans-scleral injection. Immunohistochemistry was employed to detect microglia and macrophages. PlX5622 feed was used to achieve microglia depletion and microglia repopulation. RNA-seq and qPCR were utilized to evaluate gene expression. Confocal microscopy was used to observe the interaction between microglia and donor photoreceptors. Results Donor photoreceptors survived in rd1 mice but not in wild-type mice after trans-scleral injection. The microglial cells closely interacted with donor cells. While donor cells failed to survive in rd1 mice after microglia depletion, they could survive following microglia repopulation. The RNA-seq analysis showed a pro-neurodevelopmental effect of sub-retinal microglia/RPE tissue in rd1 mice. Conclusions Subretinal microglia supported donor photoreceptor survival in rd1 mice.https://doi.org/10.1186/s13287-024-04052-0Subretinal microgliaPhotoreceptor transplantationInherited retinal degeneration |
| spellingShingle | Qinjia Ren Fang Lu Ruwa Hao Yingying Chen Chen Liang Subretinal microglia support donor photoreceptor survival in rd1 mice Stem Cell Research & Therapy Subretinal microglia Photoreceptor transplantation Inherited retinal degeneration |
| title | Subretinal microglia support donor photoreceptor survival in rd1 mice |
| title_full | Subretinal microglia support donor photoreceptor survival in rd1 mice |
| title_fullStr | Subretinal microglia support donor photoreceptor survival in rd1 mice |
| title_full_unstemmed | Subretinal microglia support donor photoreceptor survival in rd1 mice |
| title_short | Subretinal microglia support donor photoreceptor survival in rd1 mice |
| title_sort | subretinal microglia support donor photoreceptor survival in rd1 mice |
| topic | Subretinal microglia Photoreceptor transplantation Inherited retinal degeneration |
| url | https://doi.org/10.1186/s13287-024-04052-0 |
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