Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells.
Calgranulin B is known to be involved in tumor development, but the underlying molecular mechanism is not clear. To gain insight into possible roles of calgranulin B, we screened for calgranulin B-interacting molecules in the SNU-484 gastric cancer and the SNU-81 colon cancer cells. Calgranulin B-in...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171232&type=printable |
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| author | Kyung-Hee Kim Seung-Gu Yeo Byong Chul Yoo Jae Kyung Myung |
| author_facet | Kyung-Hee Kim Seung-Gu Yeo Byong Chul Yoo Jae Kyung Myung |
| author_sort | Kyung-Hee Kim |
| collection | DOAJ |
| description | Calgranulin B is known to be involved in tumor development, but the underlying molecular mechanism is not clear. To gain insight into possible roles of calgranulin B, we screened for calgranulin B-interacting molecules in the SNU-484 gastric cancer and the SNU-81 colon cancer cells. Calgranulin B-interacting partners were identified by yeast two-hybrid and functional information was obtained by computational analysis. Most of the calgranulin B-interacting partners were involved in metabolic and cellular processes, and found to have molecular function of binding and catalytic activities. Interestingly, 46 molecules in the network of the calgranulin B-interacting proteins are known to be associated with cancer and FKBP2 was found to interact with calgranulin B in both SNU-484 and SNU-81 cells. Polyubiquitin-C encoded by UBC, which exhibited an interaction with calgranulin B, has been associated with various molecules of the extracellular space and plasma membrane identified in our screening, including Na-K-Cl cotransporter 1 and dystonin in SNU-484 cells, and ATPase subunit beta-1 in SNU-81 cells. Our data provide novel insight into the roles of calgranulin B of gastrointestinal cancer cells, and offer new clues suggesting calgranulin B acts as an effector molecule through which the cell can communicate with the tumor microenvironment via polyubiquitin-C. |
| format | Article |
| id | doaj-art-9b1eb9c8bfa04ae4b2abca396bdec0c9 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-9b1eb9c8bfa04ae4b2abca396bdec0c92025-08-20T02:03:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017123210.1371/journal.pone.0171232Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells.Kyung-Hee KimSeung-Gu YeoByong Chul YooJae Kyung MyungCalgranulin B is known to be involved in tumor development, but the underlying molecular mechanism is not clear. To gain insight into possible roles of calgranulin B, we screened for calgranulin B-interacting molecules in the SNU-484 gastric cancer and the SNU-81 colon cancer cells. Calgranulin B-interacting partners were identified by yeast two-hybrid and functional information was obtained by computational analysis. Most of the calgranulin B-interacting partners were involved in metabolic and cellular processes, and found to have molecular function of binding and catalytic activities. Interestingly, 46 molecules in the network of the calgranulin B-interacting proteins are known to be associated with cancer and FKBP2 was found to interact with calgranulin B in both SNU-484 and SNU-81 cells. Polyubiquitin-C encoded by UBC, which exhibited an interaction with calgranulin B, has been associated with various molecules of the extracellular space and plasma membrane identified in our screening, including Na-K-Cl cotransporter 1 and dystonin in SNU-484 cells, and ATPase subunit beta-1 in SNU-81 cells. Our data provide novel insight into the roles of calgranulin B of gastrointestinal cancer cells, and offer new clues suggesting calgranulin B acts as an effector molecule through which the cell can communicate with the tumor microenvironment via polyubiquitin-C.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171232&type=printable |
| spellingShingle | Kyung-Hee Kim Seung-Gu Yeo Byong Chul Yoo Jae Kyung Myung Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells. PLoS ONE |
| title | Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells. |
| title_full | Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells. |
| title_fullStr | Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells. |
| title_full_unstemmed | Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells. |
| title_short | Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells. |
| title_sort | identification of calgranulin b interacting proteins and network analysis in gastrointestinal cancer cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171232&type=printable |
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