The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil

Excessive use of chemical fertilizers and extensive farming can degrade soil properties so that leading to decline in crop yields. Combining plant growth-promoting rhizobacteria (PGPR) with biochar (BC) may be an alternative way to mitigate this situation. However, the proportion of PGPR and BC at w...

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Main Authors: Qianmei Zou, Longyuan Zhao, Lirong Guan, Ping Chen, Jie Zhao, Yueying Zhao, Yunlong Du, Yong Xie
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2024.1501400/full
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author Qianmei Zou
Longyuan Zhao
Lirong Guan
Ping Chen
Jie Zhao
Yueying Zhao
Yunlong Du
Yunlong Du
Yong Xie
Yong Xie
author_facet Qianmei Zou
Longyuan Zhao
Lirong Guan
Ping Chen
Jie Zhao
Yueying Zhao
Yunlong Du
Yunlong Du
Yong Xie
Yong Xie
author_sort Qianmei Zou
collection DOAJ
description Excessive use of chemical fertilizers and extensive farming can degrade soil properties so that leading to decline in crop yields. Combining plant growth-promoting rhizobacteria (PGPR) with biochar (BC) may be an alternative way to mitigate this situation. However, the proportion of PGPR and BC at which crop yield can be improved, as well as the improvement effect extent on different eco-geographic region and crops, remain unclear. This research used cabbage [Brassica pekinensis (Lour.) Rupr.] as the target crop and established as treatment conventional fertilization as a control and a 50% reduction in nitrogen fertilizer at the Yunnan-Guizhou Plateau of China, adding BC or PGPR to evaluate the effects of different treatments on cabbage yield and the soil physicochemical properties. Specifically, high-throughput sequencing probed beneficial soil microbial communities and investigated the impact of BC and PGPR on cabbage yield and soil properties. The results revealed that the soil alkaline hydrolyzable nitrogen (AH-N), available phosphorus (AP), and available potassium (AK) contents were higher in the BC application than in control. The BC application or mixed with PGPR significantly increased the soil organic matter (OM) content (P<0.05), with a maximum of 42.59 g/kg. Further, applying BC or PGPR significantly increased the abundance of beneficial soil microorganisms in the whole growth period of cabbage (P<0.05), such as Streptomyces, Lysobacter, and Bacillus. Meanwhile, the co-application of BC and PGPR increased the abundance of Pseudomonas, and also significantly enhanced the Shannon index and Simpson index of bacterial community (P<0.05). Combined or not with PGPR, the BC application significantly enhanced cabbage yield (P<0.05), with the highest yield reached 1.41 fold of the control. Our research indicated that BC is an suitable and promising carrier of PGPR for soil improvement, combining BC and PGPR can effectively ameliorate the diversity of bacterial community even in acid red soil rhizosphere, and the most direct reflection is to improve soil fertility and cabbage yield.
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spelling doaj-art-9b1bcb7bf263461bb137512e26499e142025-08-20T01:58:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.15014001501400The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soilQianmei Zou0Longyuan Zhao1Lirong Guan2Ping Chen3Jie Zhao4Yueying Zhao5Yunlong Du6Yunlong Du7Yong Xie8Yong Xie9College of Plant Protection, Yunnan Agricultural University, Kunming, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, ChinaCollege of Chemical Engineering, Yunnan Open University, Kunming, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, ChinaExcessive use of chemical fertilizers and extensive farming can degrade soil properties so that leading to decline in crop yields. Combining plant growth-promoting rhizobacteria (PGPR) with biochar (BC) may be an alternative way to mitigate this situation. However, the proportion of PGPR and BC at which crop yield can be improved, as well as the improvement effect extent on different eco-geographic region and crops, remain unclear. This research used cabbage [Brassica pekinensis (Lour.) Rupr.] as the target crop and established as treatment conventional fertilization as a control and a 50% reduction in nitrogen fertilizer at the Yunnan-Guizhou Plateau of China, adding BC or PGPR to evaluate the effects of different treatments on cabbage yield and the soil physicochemical properties. Specifically, high-throughput sequencing probed beneficial soil microbial communities and investigated the impact of BC and PGPR on cabbage yield and soil properties. The results revealed that the soil alkaline hydrolyzable nitrogen (AH-N), available phosphorus (AP), and available potassium (AK) contents were higher in the BC application than in control. The BC application or mixed with PGPR significantly increased the soil organic matter (OM) content (P<0.05), with a maximum of 42.59 g/kg. Further, applying BC or PGPR significantly increased the abundance of beneficial soil microorganisms in the whole growth period of cabbage (P<0.05), such as Streptomyces, Lysobacter, and Bacillus. Meanwhile, the co-application of BC and PGPR increased the abundance of Pseudomonas, and also significantly enhanced the Shannon index and Simpson index of bacterial community (P<0.05). Combined or not with PGPR, the BC application significantly enhanced cabbage yield (P<0.05), with the highest yield reached 1.41 fold of the control. Our research indicated that BC is an suitable and promising carrier of PGPR for soil improvement, combining BC and PGPR can effectively ameliorate the diversity of bacterial community even in acid red soil rhizosphere, and the most direct reflection is to improve soil fertility and cabbage yield.https://www.frontiersin.org/articles/10.3389/fpls.2024.1501400/fullbeneficial microbial communitiesbiological agentsrhizosphere microorganismssoil improvementsoil physicochemical properties
spellingShingle Qianmei Zou
Longyuan Zhao
Lirong Guan
Ping Chen
Jie Zhao
Yueying Zhao
Yunlong Du
Yunlong Du
Yong Xie
Yong Xie
The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil
Frontiers in Plant Science
beneficial microbial communities
biological agents
rhizosphere microorganisms
soil improvement
soil physicochemical properties
title The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil
title_full The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil
title_fullStr The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil
title_full_unstemmed The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil
title_short The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil
title_sort synergistic interaction effect between biochar and plant growth promoting rhizobacteria on beneficial microbial communities in soil
topic beneficial microbial communities
biological agents
rhizosphere microorganisms
soil improvement
soil physicochemical properties
url https://www.frontiersin.org/articles/10.3389/fpls.2024.1501400/full
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