Antibacterial action of novel zeolite-based compositions depends upon doping with Ag(+) and Сu(2+) cations
Recently, there is a growing interest to exploration of sorption and catalytic properties of solid nanomaterials, in particular natural zeolites, as well as to study of their antimicrobial effects with the aim of potential using them as a principal component of disinfection and degassing remedies. T...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2024-10-01
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| Series: | The Ukrainian Biochemical Journal |
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| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2024/10/Manko_96_5.pdf |
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| author | N. O. Manko O. O. Ilkov O. Yu. Klyuchivska V. O. Vasylechko V. V. Sydorchuk N. P. Kovalska O. I. Kostiv S. R. Bagday A. V. Zelinskiy O. M. Gromyko N. V. Skrypchenko Yа. M. Kalychak R. S. Stoika |
| author_facet | N. O. Manko O. O. Ilkov O. Yu. Klyuchivska V. O. Vasylechko V. V. Sydorchuk N. P. Kovalska O. I. Kostiv S. R. Bagday A. V. Zelinskiy O. M. Gromyko N. V. Skrypchenko Yа. M. Kalychak R. S. Stoika |
| author_sort | N. O. Manko |
| collection | DOAJ |
| description | Recently, there is a growing interest to exploration of sorption and catalytic properties of solid nanomaterials, in particular natural zeolites, as well as to study of their antimicrobial effects with the aim of potential using them as a principal component of disinfection and degassing remedies. The purpose of this work was to study the antimicrobial action of compositions based on the Transcarpathian clinoptilolite (CL) doped with Ag+ and Сu2+ cations or Ag microparticles (MPs). These compositions were subjected to mechanochemical modification in ethanol medium and with the addition of plant (Actinidia arguta) extract used as an antioxidant. Mechanochemical treatment (MChT) of all forms of CL MPs led to their grinding which caused better contact of CL with bacterial cells, while an increased content of larger pores improved their access to the active sites on the surface of the CL MPs. Treatment of CL samples with metallic silver used as a dopant with the help of the extract of Actinidia arguta plant did not increase the antibacterial activity regardless of treatment time. Treatment of AgNO3 with ethanol slightly increased the antibacterial action of the CL MPs towards Gram-positive bacteria and decreased it towards Gram-negative bacteria. The CL samples doped with copper and treated with ethanol and plant (Actinidia arguta) extract demonstrated comparable toxic action towards Bacillus subtilis regardless of grinding conditions. While such a treatment caused a significant decrease in the antibacterial activity towards Staphylococcus aureus and Pseudomonas aeruginosa strains, compared to the action of samples that were not treated with that plant extract. To address the potential biochemical mechanisms of the antibacterial action of the created zeolite-based compositions, their influence on generation of the reactive oxygen species (ROS) was studied using diphenylpicrylhydrazyl (DPH) fluorescent dye. Most versions of the CL composites demonstrated time-dependent antioxidant effect comparable with the effect of the ascorbic acid used as a positive control. Thus, the ROS generation is not the mechanism that is responsible for the antibacterial action of the created CL-based compositions. Probably, that action is explained by the peculiarities of interaction of doped CL microparticles with the surface of the bacterial cells. |
| format | Article |
| id | doaj-art-9b0df774b2a846dabc463102606d145b |
| institution | DOAJ |
| issn | 2409-4943 2413-5003 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj-art-9b0df774b2a846dabc463102606d145b2025-08-20T03:10:10ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032024-10-0196510411810.15407/ubj96.05.104Antibacterial action of novel zeolite-based compositions depends upon doping with Ag(+) and Сu(2+) cationsN. O. Manko0https://orcid.org/0000-0003-1203-7479 O. O. Ilkov1 O. Yu. Klyuchivska2https://orcid.org/0000-0001-8657-5945V. O. Vasylechko3https://orcid.org/0000-0003-1229-9313V. V. Sydorchuk4https://orcid.org/0000-0003-0967-8750N. P. KovalskaO. I. Kostiv5https://orcid.org/0000-0003-4441-8428S. R. Bagday6https://orcid.org/0009-0003-4414-7944A. V. Zelinskiy7https://orcid.org/0000-0001-8473-1174 O. M. Gromyko8https://orcid.org/0000-0002-8107-0128 N. V. Skrypchenko9https://orcid.org/0000-0002-3311-6654Yа. M. Kalychak10https://orcid.org/0000-0003-4863-315XR. S. Stoika11https://orcid.org/0000-0001-5719-2187Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, LvivHalychpharm, Lviv, UkraineDepartment of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, LvivFaculty of Chemistry, Ivan Franko National University of Lviv, Lviv, Ukraine; Department of Food Technology, Lviv University of Trade and Economics, Lviv, UkraineDepartment of Heterogeneous Catalytic Oxidation Processes, Institute of Sorption and Problems of Endoecology, National Academy of Sciences of Ukraine, Kyiv, UkraineFaculty of Chemistry, Ivan Franko National University of Lviv, Lviv, UkraineFaculty of Chemistry, Ivan Franko National University of Lviv, Lviv, UkraineFaculty of Chemistry, Ivan Franko National University of Lviv, Lviv, UkraineFaculty of Biology, Ivan Franko National University of Lviv, Lviv, UkraineDepartment of Fruit Plants Acclimatization, M. M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine, KyivFaculty of Chemistry, Ivan Franko National University of Lviv, Lviv, UkraineDepartment of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, LvivRecently, there is a growing interest to exploration of sorption and catalytic properties of solid nanomaterials, in particular natural zeolites, as well as to study of their antimicrobial effects with the aim of potential using them as a principal component of disinfection and degassing remedies. The purpose of this work was to study the antimicrobial action of compositions based on the Transcarpathian clinoptilolite (CL) doped with Ag+ and Сu2+ cations or Ag microparticles (MPs). These compositions were subjected to mechanochemical modification in ethanol medium and with the addition of plant (Actinidia arguta) extract used as an antioxidant. Mechanochemical treatment (MChT) of all forms of CL MPs led to their grinding which caused better contact of CL with bacterial cells, while an increased content of larger pores improved their access to the active sites on the surface of the CL MPs. Treatment of CL samples with metallic silver used as a dopant with the help of the extract of Actinidia arguta plant did not increase the antibacterial activity regardless of treatment time. Treatment of AgNO3 with ethanol slightly increased the antibacterial action of the CL MPs towards Gram-positive bacteria and decreased it towards Gram-negative bacteria. The CL samples doped with copper and treated with ethanol and plant (Actinidia arguta) extract demonstrated comparable toxic action towards Bacillus subtilis regardless of grinding conditions. While such a treatment caused a significant decrease in the antibacterial activity towards Staphylococcus aureus and Pseudomonas aeruginosa strains, compared to the action of samples that were not treated with that plant extract. To address the potential biochemical mechanisms of the antibacterial action of the created zeolite-based compositions, their influence on generation of the reactive oxygen species (ROS) was studied using diphenylpicrylhydrazyl (DPH) fluorescent dye. Most versions of the CL composites demonstrated time-dependent antioxidant effect comparable with the effect of the ascorbic acid used as a positive control. Thus, the ROS generation is not the mechanism that is responsible for the antibacterial action of the created CL-based compositions. Probably, that action is explained by the peculiarities of interaction of doped CL microparticles with the surface of the bacterial cells.http://ukrbiochemjournal.org/wp-content/uploads/2024/10/Manko_96_5.pdfantimicrobial actionclinoptilolite-based compositionsdoping with ag+ and сu2+ cationsphysicochemical treatment |
| spellingShingle | N. O. Manko O. O. Ilkov O. Yu. Klyuchivska V. O. Vasylechko V. V. Sydorchuk N. P. Kovalska O. I. Kostiv S. R. Bagday A. V. Zelinskiy O. M. Gromyko N. V. Skrypchenko Yа. M. Kalychak R. S. Stoika Antibacterial action of novel zeolite-based compositions depends upon doping with Ag(+) and Сu(2+) cations The Ukrainian Biochemical Journal antimicrobial action clinoptilolite-based compositions doping with ag+ and сu2+ cations physicochemical treatment |
| title | Antibacterial action of novel zeolite-based compositions depends upon doping with Ag(+) and Сu(2+) cations |
| title_full | Antibacterial action of novel zeolite-based compositions depends upon doping with Ag(+) and Сu(2+) cations |
| title_fullStr | Antibacterial action of novel zeolite-based compositions depends upon doping with Ag(+) and Сu(2+) cations |
| title_full_unstemmed | Antibacterial action of novel zeolite-based compositions depends upon doping with Ag(+) and Сu(2+) cations |
| title_short | Antibacterial action of novel zeolite-based compositions depends upon doping with Ag(+) and Сu(2+) cations |
| title_sort | antibacterial action of novel zeolite based compositions depends upon doping with ag and сu 2 cations |
| topic | antimicrobial action clinoptilolite-based compositions doping with ag+ and сu2+ cations physicochemical treatment |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2024/10/Manko_96_5.pdf |
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