Single‐Virus Stochastic Biosensing: Proof of Concept for SARS‐CoV‐2 Detection in Complex Medium Using CMOS‐Based Nanocapacitor Arrays
Abstract Stochastic detection opens a promising window toward improved biosensing assays, despite the challenges posed by the unpredictable behavior of nanoscale entities as well as interference from the target medium. This study presents a novel proof of concept for label‐free detection of single v...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-08-01
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| Series: | Advanced Sensor Research |
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| Online Access: | https://doi.org/10.1002/adsr.202400193 |
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| author | Suryasnata Tripathy Mohammad Saghafi Sudip Kumar Dutta Stijn van derHam Diogenis Salvanos Cecilia Laborde Byron Martina Serge G. Lemay |
| author_facet | Suryasnata Tripathy Mohammad Saghafi Sudip Kumar Dutta Stijn van derHam Diogenis Salvanos Cecilia Laborde Byron Martina Serge G. Lemay |
| author_sort | Suryasnata Tripathy |
| collection | DOAJ |
| description | Abstract Stochastic detection opens a promising window toward improved biosensing assays, despite the challenges posed by the unpredictable behavior of nanoscale entities as well as interference from the target medium. This study presents a novel proof of concept for label‐free detection of single virus particles in complex media at physiological salt concentrations using stochastic electrochemical impedance. SARS‐CoV‐2 particles are successfully detected in cell culture medium using thiolated aptamers that selectively bind to the virus's spike S1 proteins, enabling the identification of individual viral particles. Stochastic biosensing, which relies on large datasets, is powered here by CMOS‐based nanocapacitor arrays with 65536 individually addressable electrodes serving as electrochemical transducers. This configuration allows for high‐frequency impedance measurements under physiological conditions, demonstrating the potential for scalable, real‐time, label‐free virus detection. |
| format | Article |
| id | doaj-art-9b0c40e15a58442fb58fd52bce345d41 |
| institution | Kabale University |
| issn | 2751-1219 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Sensor Research |
| spelling | doaj-art-9b0c40e15a58442fb58fd52bce345d412025-08-20T03:59:32ZengWiley-VCHAdvanced Sensor Research2751-12192025-08-0148n/an/a10.1002/adsr.202400193Single‐Virus Stochastic Biosensing: Proof of Concept for SARS‐CoV‐2 Detection in Complex Medium Using CMOS‐Based Nanocapacitor ArraysSuryasnata Tripathy0Mohammad Saghafi1Sudip Kumar Dutta2Stijn van derHam3Diogenis Salvanos4Cecilia Laborde5Byron Martina6Serge G. Lemay7MESA+ Institute for Nanotechnology and Faculty of Science and Technology University of Twente P.O. Box 217 AE Enschede 7500 The NetherlandsMESA+ Institute for Nanotechnology and Faculty of Science and Technology University of Twente P.O. Box 217 AE Enschede 7500 The NetherlandsArtemis Bioservices Molengraaffsingel 10 JD Delft 2629 The NetherlandsMESA+ Institute for Nanotechnology and Faculty of Science and Technology University of Twente P.O. Box 217 AE Enschede 7500 The NetherlandsMESA+ Institute for Nanotechnology and Faculty of Science and Technology University of Twente P.O. Box 217 AE Enschede 7500 The NetherlandsMESA+ Institute for Nanotechnology and Faculty of Science and Technology University of Twente P.O. Box 217 AE Enschede 7500 The NetherlandsArtemis Bioservices Molengraaffsingel 10 JD Delft 2629 The NetherlandsMESA+ Institute for Nanotechnology and Faculty of Science and Technology University of Twente P.O. Box 217 AE Enschede 7500 The NetherlandsAbstract Stochastic detection opens a promising window toward improved biosensing assays, despite the challenges posed by the unpredictable behavior of nanoscale entities as well as interference from the target medium. This study presents a novel proof of concept for label‐free detection of single virus particles in complex media at physiological salt concentrations using stochastic electrochemical impedance. SARS‐CoV‐2 particles are successfully detected in cell culture medium using thiolated aptamers that selectively bind to the virus's spike S1 proteins, enabling the identification of individual viral particles. Stochastic biosensing, which relies on large datasets, is powered here by CMOS‐based nanocapacitor arrays with 65536 individually addressable electrodes serving as electrochemical transducers. This configuration allows for high‐frequency impedance measurements under physiological conditions, demonstrating the potential for scalable, real‐time, label‐free virus detection.https://doi.org/10.1002/adsr.202400193CMOSelectrochemical impedance spectroscopynanoelectrodesSARS‐CoV‐2stochastic biosensing |
| spellingShingle | Suryasnata Tripathy Mohammad Saghafi Sudip Kumar Dutta Stijn van derHam Diogenis Salvanos Cecilia Laborde Byron Martina Serge G. Lemay Single‐Virus Stochastic Biosensing: Proof of Concept for SARS‐CoV‐2 Detection in Complex Medium Using CMOS‐Based Nanocapacitor Arrays Advanced Sensor Research CMOS electrochemical impedance spectroscopy nanoelectrodes SARS‐CoV‐2 stochastic biosensing |
| title | Single‐Virus Stochastic Biosensing: Proof of Concept for SARS‐CoV‐2 Detection in Complex Medium Using CMOS‐Based Nanocapacitor Arrays |
| title_full | Single‐Virus Stochastic Biosensing: Proof of Concept for SARS‐CoV‐2 Detection in Complex Medium Using CMOS‐Based Nanocapacitor Arrays |
| title_fullStr | Single‐Virus Stochastic Biosensing: Proof of Concept for SARS‐CoV‐2 Detection in Complex Medium Using CMOS‐Based Nanocapacitor Arrays |
| title_full_unstemmed | Single‐Virus Stochastic Biosensing: Proof of Concept for SARS‐CoV‐2 Detection in Complex Medium Using CMOS‐Based Nanocapacitor Arrays |
| title_short | Single‐Virus Stochastic Biosensing: Proof of Concept for SARS‐CoV‐2 Detection in Complex Medium Using CMOS‐Based Nanocapacitor Arrays |
| title_sort | single virus stochastic biosensing proof of concept for sars cov 2 detection in complex medium using cmos based nanocapacitor arrays |
| topic | CMOS electrochemical impedance spectroscopy nanoelectrodes SARS‐CoV‐2 stochastic biosensing |
| url | https://doi.org/10.1002/adsr.202400193 |
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