Fluorescent probes to track complex membrane blebbing
Cellular blebbing, pivotal in processes such as apoptosis, cytokinesis, and migration, involves dynamic interactions between the actomyosin network and microtubules. However, existing probes inadequately capture these simultaneous interactions, limiting our ability to study blebbing mechanisms. We p...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Biosensors and Bioelectronics: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137025000111 |
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| author | Juan L. Cortes-Muñoz Johan-Moritz Kux Pablo J. Sáez Arturo Jiménez-Sánchez |
| author_facet | Juan L. Cortes-Muñoz Johan-Moritz Kux Pablo J. Sáez Arturo Jiménez-Sánchez |
| author_sort | Juan L. Cortes-Muñoz |
| collection | DOAJ |
| description | Cellular blebbing, pivotal in processes such as apoptosis, cytokinesis, and migration, involves dynamic interactions between the actomyosin network and microtubules. However, existing probes inadequately capture these simultaneous interactions, limiting our ability to study blebbing mechanisms. We present AztecBleb probes (AztecBlebDAPI, AztecBlebGFP, AztecBlebTxR, AztecBlebCy5), novel fluorescent reporters designed to selectively target and monitor blebbing in real-time. These probes incorporate a pregnenolone-based scaffold as a hydrophobic core derived from abiraterone acetate, facilitating precise localization to blebs and microtubules without disrupting cellular function. Through persistent staining of cell blebs, these photostable and biocompatible probes enable continuous monitoring of blebbing and microtubule dynamics during cellular migration. Our approach provides new insights into the coordination of bleb formation and cytoskeletal remodeling, offering a unique tool for studying motility-driven cellular behaviors. |
| format | Article |
| id | doaj-art-9b06ae623c34498ebed906eeaba53d42 |
| institution | OA Journals |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-9b06ae623c34498ebed906eeaba53d422025-08-20T02:06:44ZengElsevierBiosensors and Bioelectronics: X2590-13702025-05-012310058410.1016/j.biosx.2025.100584Fluorescent probes to track complex membrane blebbingJuan L. Cortes-Muñoz0Johan-Moritz Kux1Pablo J. Sáez2Arturo Jiménez-Sánchez3Bioorganic Chemistry Laboratory (BioChela) at Instituto de Química. Universidad Nacional Autónoma de México (UNAM), Circuito Exterior s/n, Coyoacán, Ciudad Universitaria, 04510, Mexico City, MexicoCellComM Lab - IBMZ - SF1328. University Medical Center Hamburg- Eppendorf | UKE, Martinistraße 52, N30, 20246, Hamburg, GermanyCellComM Lab - IBMZ - SF1328. University Medical Center Hamburg- Eppendorf | UKE, Martinistraße 52, N30, 20246, Hamburg, Germany; Corresponding author.Bioorganic Chemistry Laboratory (BioChela) at Instituto de Química. Universidad Nacional Autónoma de México (UNAM), Circuito Exterior s/n, Coyoacán, Ciudad Universitaria, 04510, Mexico City, Mexico; Corresponding author.Cellular blebbing, pivotal in processes such as apoptosis, cytokinesis, and migration, involves dynamic interactions between the actomyosin network and microtubules. However, existing probes inadequately capture these simultaneous interactions, limiting our ability to study blebbing mechanisms. We present AztecBleb probes (AztecBlebDAPI, AztecBlebGFP, AztecBlebTxR, AztecBlebCy5), novel fluorescent reporters designed to selectively target and monitor blebbing in real-time. These probes incorporate a pregnenolone-based scaffold as a hydrophobic core derived from abiraterone acetate, facilitating precise localization to blebs and microtubules without disrupting cellular function. Through persistent staining of cell blebs, these photostable and biocompatible probes enable continuous monitoring of blebbing and microtubule dynamics during cellular migration. Our approach provides new insights into the coordination of bleb formation and cytoskeletal remodeling, offering a unique tool for studying motility-driven cellular behaviors.http://www.sciencedirect.com/science/article/pii/S2590137025000111Cell blebbingCell migrationFluorescent probesFluorophore-targetingCytoskeleton dynamics |
| spellingShingle | Juan L. Cortes-Muñoz Johan-Moritz Kux Pablo J. Sáez Arturo Jiménez-Sánchez Fluorescent probes to track complex membrane blebbing Biosensors and Bioelectronics: X Cell blebbing Cell migration Fluorescent probes Fluorophore-targeting Cytoskeleton dynamics |
| title | Fluorescent probes to track complex membrane blebbing |
| title_full | Fluorescent probes to track complex membrane blebbing |
| title_fullStr | Fluorescent probes to track complex membrane blebbing |
| title_full_unstemmed | Fluorescent probes to track complex membrane blebbing |
| title_short | Fluorescent probes to track complex membrane blebbing |
| title_sort | fluorescent probes to track complex membrane blebbing |
| topic | Cell blebbing Cell migration Fluorescent probes Fluorophore-targeting Cytoskeleton dynamics |
| url | http://www.sciencedirect.com/science/article/pii/S2590137025000111 |
| work_keys_str_mv | AT juanlcortesmunoz fluorescentprobestotrackcomplexmembraneblebbing AT johanmoritzkux fluorescentprobestotrackcomplexmembraneblebbing AT pablojsaez fluorescentprobestotrackcomplexmembraneblebbing AT arturojimenezsanchez fluorescentprobestotrackcomplexmembraneblebbing |