Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy
Sindbis virus (SINV) is an alphavirus that has a broad host range and has been widely used as a vector for recombinant gene transduction, DNA-based vaccine production, and oncolytic cancer therapy. The mechanism of SINV entry into host cells has yet to be fully understood. In this paper, we used sin...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2011/535206 |
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| author | Youling Gu Yuanzheng Yang Yuechueng Liu |
| author_facet | Youling Gu Yuanzheng Yang Yuechueng Liu |
| author_sort | Youling Gu |
| collection | DOAJ |
| description | Sindbis virus (SINV) is an alphavirus that has a broad host range and has been widely used as a vector for recombinant gene transduction, DNA-based vaccine production, and oncolytic cancer therapy. The mechanism of SINV entry into host cells has yet to be fully understood. In this paper, we used single virus tracking under total internal reflection fluorescence microscopy (TIRFM) to investigate SINV attachment to cell surface. Biotinylated viral particles were labeled with quantum dots, which retained viral viability and infectivity. By time-lapse imaging, we showed that the SINV exhibited a heterogeneous dynamics on the surface of the host cells. Analysis of SINV motility demonstrated a two-step attachment reaction. Moreover, dual color TIRFM of GFP-Rab5 and SINV suggested that the virus was targeted to the early endosomes after endocytosis. These findings demonstrate the utility of quantum dot labeling in studying the early steps and behavior of SINV infection. |
| format | Article |
| id | doaj-art-9afd854058e34b429fd60f9a66db69b9 |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-9afd854058e34b429fd60f9a66db69b92025-02-03T01:30:32ZengWileyAdvances in Virology1687-86391687-86472011-01-01201110.1155/2011/535206535206Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence MicroscopyYouling Gu0Yuanzheng Yang1Yuechueng Liu2Department of Pathology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, Ok 73190, USADepartment of Pathology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, Ok 73190, USADepartment of Pathology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, Ok 73190, USASindbis virus (SINV) is an alphavirus that has a broad host range and has been widely used as a vector for recombinant gene transduction, DNA-based vaccine production, and oncolytic cancer therapy. The mechanism of SINV entry into host cells has yet to be fully understood. In this paper, we used single virus tracking under total internal reflection fluorescence microscopy (TIRFM) to investigate SINV attachment to cell surface. Biotinylated viral particles were labeled with quantum dots, which retained viral viability and infectivity. By time-lapse imaging, we showed that the SINV exhibited a heterogeneous dynamics on the surface of the host cells. Analysis of SINV motility demonstrated a two-step attachment reaction. Moreover, dual color TIRFM of GFP-Rab5 and SINV suggested that the virus was targeted to the early endosomes after endocytosis. These findings demonstrate the utility of quantum dot labeling in studying the early steps and behavior of SINV infection.http://dx.doi.org/10.1155/2011/535206 |
| spellingShingle | Youling Gu Yuanzheng Yang Yuechueng Liu Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy Advances in Virology |
| title | Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy |
| title_full | Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy |
| title_fullStr | Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy |
| title_full_unstemmed | Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy |
| title_short | Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy |
| title_sort | imaging early steps of sindbis virus infection by total internal reflection fluorescence microscopy |
| url | http://dx.doi.org/10.1155/2011/535206 |
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