Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave
For two libraries (PDF1 and ODF1) using Illumina sequencing 44,082,301 and 43,708,132 clean reads were obtained, respectively. After being mapped to the bovine RefSeq database, 15,533 genes were identified to be expressed in both types of follicles (cut-off RPKM > 0.5), of which 719 were highly e...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2016/3472748 |
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| author | Pengfei Li Jinzhu Meng Wenzhong Liu George W. Smith Jianbo Yao Lihua Lyu |
| author_facet | Pengfei Li Jinzhu Meng Wenzhong Liu George W. Smith Jianbo Yao Lihua Lyu |
| author_sort | Pengfei Li |
| collection | DOAJ |
| description | For two libraries (PDF1 and ODF1) using Illumina sequencing 44,082,301 and 43,708,132 clean reads were obtained, respectively. After being mapped to the bovine RefSeq database, 15,533 genes were identified to be expressed in both types of follicles (cut-off RPKM > 0.5), of which 719 were highly expressed in bovine follicles (cut-off RPKM > 100). Furthermore, 83 genes were identified as being differentially expressed in ODF1 versus PDF1, where 42 genes were upregulated and 41 genes were downregulated. KEGG pathway analysis revealed two upregulated genes in ODF1 versus PDF1, CYP11A1, and CYP19A1, which are important genes in the steroid hormone biosynthesis pathway. This study represents the first investigation of transcriptome of bovine follicles at predeviation and onset of deviation stages and provides a foundation for future investigation of the regulatory mechanisms involved in follicular development in cattle. |
| format | Article |
| id | doaj-art-9ae1c5f4b2e7472ebbc1463aa22c4deb |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-9ae1c5f4b2e7472ebbc1463aa22c4deb2025-02-03T01:24:11ZengWileyInternational Journal of Genomics2314-436X2314-43782016-01-01201610.1155/2016/34727483472748Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular WavePengfei Li0Jinzhu Meng1Wenzhong Liu2George W. Smith3Jianbo Yao4Lihua Lyu5College of Life Science, Shanxi Agricultural University, Taigu, Shanxi 030801, ChinaCollege of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, ChinaCollege of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, ChinaLaboratory of Mammalian Reproductive Biology and Genomics, Departments of Animal Science and Physiology, Michigan State University, East Lansing, MI 48824, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506, USACollege of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, ChinaFor two libraries (PDF1 and ODF1) using Illumina sequencing 44,082,301 and 43,708,132 clean reads were obtained, respectively. After being mapped to the bovine RefSeq database, 15,533 genes were identified to be expressed in both types of follicles (cut-off RPKM > 0.5), of which 719 were highly expressed in bovine follicles (cut-off RPKM > 100). Furthermore, 83 genes were identified as being differentially expressed in ODF1 versus PDF1, where 42 genes were upregulated and 41 genes were downregulated. KEGG pathway analysis revealed two upregulated genes in ODF1 versus PDF1, CYP11A1, and CYP19A1, which are important genes in the steroid hormone biosynthesis pathway. This study represents the first investigation of transcriptome of bovine follicles at predeviation and onset of deviation stages and provides a foundation for future investigation of the regulatory mechanisms involved in follicular development in cattle.http://dx.doi.org/10.1155/2016/3472748 |
| spellingShingle | Pengfei Li Jinzhu Meng Wenzhong Liu George W. Smith Jianbo Yao Lihua Lyu Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave International Journal of Genomics |
| title | Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave |
| title_full | Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave |
| title_fullStr | Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave |
| title_full_unstemmed | Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave |
| title_short | Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave |
| title_sort | transcriptome analysis of bovine ovarian follicles at predeviation and onset of deviation stages of a follicular wave |
| url | http://dx.doi.org/10.1155/2016/3472748 |
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