Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase
Abstract Influenza A virus neuraminidase (NA) is drawing attention as a target for vaccine development. In this study, we propose kinetic MUNANA assay as a tool to identify monoclonal antibodies (mAbs) that specifically target functional epitopes on NA. By analyzing changes in the parameters of the...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | npj Viruses |
| Online Access: | https://doi.org/10.1038/s44298-025-00123-y |
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| author | Ilya V. Smirnov Danica F. Besavilla Karin Schön Hannes Axelsson Davide Angeletti |
| author_facet | Ilya V. Smirnov Danica F. Besavilla Karin Schön Hannes Axelsson Davide Angeletti |
| author_sort | Ilya V. Smirnov |
| collection | DOAJ |
| description | Abstract Influenza A virus neuraminidase (NA) is drawing attention as a target for vaccine development. In this study, we propose kinetic MUNANA assay as a tool to identify monoclonal antibodies (mAbs) that specifically target functional epitopes on NA. By analyzing changes in the parameters of the Michaelis-Menten curve (Km and Vmax), we revealed distinct mechanisms of Ab-mediated inhibition. Additionally, we developed a web-based application facilitating efficient processing of the assay results and enabling statistical inference. We employed the kinetic MUNANA assay to test newly developed mAbs targeting NA of the widely used PR8 H1N1 strain. Among these, mAbs with strong effect on NA enzymatic parameters were more likely to select for escape mutants that had a substantial impact on the overall enzymatic activity of the virus. In summary, when combined with ELLA, kinetic MUNANA is a rapid method to profile the putative binding site and the effect of NA-specific mAbs. |
| format | Article |
| id | doaj-art-9ad91d936111477683edce6535ae641a |
| institution | OA Journals |
| issn | 2948-1767 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Viruses |
| spelling | doaj-art-9ad91d936111477683edce6535ae641a2025-08-20T02:15:25ZengNature Portfolionpj Viruses2948-17672025-05-013111110.1038/s44298-025-00123-yKinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidaseIlya V. Smirnov0Danica F. Besavilla1Karin Schön2Hannes Axelsson3Davide Angeletti4Department of Microbiology and Immunology, Institute of Biomedicine, University of GothenburgDepartment of Microbiology and Immunology, Institute of Biomedicine, University of GothenburgDepartment of Microbiology and Immunology, Institute of Biomedicine, University of GothenburgDepartment of Microbiology and Immunology, Institute of Biomedicine, University of GothenburgDepartment of Microbiology and Immunology, Institute of Biomedicine, University of GothenburgAbstract Influenza A virus neuraminidase (NA) is drawing attention as a target for vaccine development. In this study, we propose kinetic MUNANA assay as a tool to identify monoclonal antibodies (mAbs) that specifically target functional epitopes on NA. By analyzing changes in the parameters of the Michaelis-Menten curve (Km and Vmax), we revealed distinct mechanisms of Ab-mediated inhibition. Additionally, we developed a web-based application facilitating efficient processing of the assay results and enabling statistical inference. We employed the kinetic MUNANA assay to test newly developed mAbs targeting NA of the widely used PR8 H1N1 strain. Among these, mAbs with strong effect on NA enzymatic parameters were more likely to select for escape mutants that had a substantial impact on the overall enzymatic activity of the virus. In summary, when combined with ELLA, kinetic MUNANA is a rapid method to profile the putative binding site and the effect of NA-specific mAbs.https://doi.org/10.1038/s44298-025-00123-y |
| spellingShingle | Ilya V. Smirnov Danica F. Besavilla Karin Schön Hannes Axelsson Davide Angeletti Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase npj Viruses |
| title | Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase |
| title_full | Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase |
| title_fullStr | Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase |
| title_full_unstemmed | Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase |
| title_short | Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase |
| title_sort | kinetic munana assay reveals functionally relevant antibody epitopes on influenza a virus neuraminidase |
| url | https://doi.org/10.1038/s44298-025-00123-y |
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