METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS
Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression and methylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expression status...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2022-08-01
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| Series: | Sabiad |
| Subjects: | |
| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/A2B085FFFDD4490982C11044F84317FB |
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| Summary: | Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression and methylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expression status of GPRC5C gene was investigated in a larger group of OSCC patients and healthy cohort for further validation. Its potential candidate biomarker was evaluated for early diagnosis of OSCC by non-invasive methods. Materials and Methods: DNA and RNA were isolated from tissues/body fluid samples of 30 patients OSCC and 15 healthy individuals. The methylation and expression levels were analyzed by using QMSP and QRT-PCR methods, respectively. The data were compared with clinical parameters and evaluated by statistical analysis. Results: Decreased expression levels were observed in 43% and increased expression levels were observed in 33% in the tumor tissues compared to corresponding-normal tissues of OSCC patients. Methylation-based expression loss was observed in 4 patients having the ratio between 3.4 and 12.2%. We also observed a decrease in expression in OSCC patients with observed methylation. There was statistical significance between differentiation degree and expression in OSCC. Conclusion: It was concluded that GPRC5C may play a role as a potential methylation biomarker candidate of specific subgroups of the oral cavity. Other mechanisms need to be explored to understand the differences in expression levels. |
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| ISSN: | 2651-4060 |