Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and Imaging
Hypochlorite (ClO−) has received extensive attention owing to its significant roles in the immune defense and pathogenesis of numerous diseases. However, excessive or misplaced production of ClO− may pose certain diseases. Thus, to determine its biological functions in depth, ClO− should be tested i...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/7515979 |
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| author | Qianchun Zhang Haijiang Du Siqi Xie Fengling Tian Xixi Long Shan Liu Yun Wu |
| author_facet | Qianchun Zhang Haijiang Du Siqi Xie Fengling Tian Xixi Long Shan Liu Yun Wu |
| author_sort | Qianchun Zhang |
| collection | DOAJ |
| description | Hypochlorite (ClO−) has received extensive attention owing to its significant roles in the immune defense and pathogenesis of numerous diseases. However, excessive or misplaced production of ClO− may pose certain diseases. Thus, to determine its biological functions in depth, ClO− should be tested in biosystems. In this study, a facile, one-pot synthesis of nitrogen-fluorine-doped carbon quantum dots (N, F-CDs) was developed using ammonium citrate tribasic, L-alanine, and ammonium fluoride as raw materials under hydrothermal conditions. The prepared N, F-CDs demonstrate not only strong blue fluorescence emission with a high fluorescence quantum yield (26.3%) but also a small particle size of approximately 2.9 nm, as well as excellent water solubility and biocompatibility. Meanwhile, the as-prepared N, F-CDs exhibit good performance in the highly selective and sensitive detection of ClO−. Thus, a wide concentration response range of 0–600 μM with a low limit of detection (0.75 μM) was favorably obtained for the N, F-CDs. Based on the excellent fluorescence stability, excellent water solubility, and low cell toxicity, the practicality and viability of the fluorescent composites were also successfully verified via detecting ClO− in water samples and living RAW 264.7 cells. The proposed probe is expected to provide a new approach for detecting ClO− in other organelles. |
| format | Article |
| id | doaj-art-9aced4d6ae494cd4931ff3bf18dfd2e5 |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-9aced4d6ae494cd4931ff3bf18dfd2e52025-08-20T03:36:53ZengWileyJournal of Analytical Methods in Chemistry2090-88732023-01-01202310.1155/2023/7515979Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and ImagingQianchun Zhang0Haijiang Du1Siqi Xie2Fengling Tian3Xixi Long4Shan Liu5Yun Wu6School of Biology and ChemistrySchool of Biology and ChemistrySchool of Biology and ChemistrySchool of Biology and ChemistrySchool of Biology and ChemistrySchool of Biology and ChemistrySchool of Biology and ChemistryHypochlorite (ClO−) has received extensive attention owing to its significant roles in the immune defense and pathogenesis of numerous diseases. However, excessive or misplaced production of ClO− may pose certain diseases. Thus, to determine its biological functions in depth, ClO− should be tested in biosystems. In this study, a facile, one-pot synthesis of nitrogen-fluorine-doped carbon quantum dots (N, F-CDs) was developed using ammonium citrate tribasic, L-alanine, and ammonium fluoride as raw materials under hydrothermal conditions. The prepared N, F-CDs demonstrate not only strong blue fluorescence emission with a high fluorescence quantum yield (26.3%) but also a small particle size of approximately 2.9 nm, as well as excellent water solubility and biocompatibility. Meanwhile, the as-prepared N, F-CDs exhibit good performance in the highly selective and sensitive detection of ClO−. Thus, a wide concentration response range of 0–600 μM with a low limit of detection (0.75 μM) was favorably obtained for the N, F-CDs. Based on the excellent fluorescence stability, excellent water solubility, and low cell toxicity, the practicality and viability of the fluorescent composites were also successfully verified via detecting ClO− in water samples and living RAW 264.7 cells. The proposed probe is expected to provide a new approach for detecting ClO− in other organelles.http://dx.doi.org/10.1155/2023/7515979 |
| spellingShingle | Qianchun Zhang Haijiang Du Siqi Xie Fengling Tian Xixi Long Shan Liu Yun Wu Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and Imaging Journal of Analytical Methods in Chemistry |
| title | Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and Imaging |
| title_full | Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and Imaging |
| title_fullStr | Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and Imaging |
| title_full_unstemmed | Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and Imaging |
| title_short | Preparation of One-Emission Nitrogen-Fluorine-Doped Carbon Quantum Dots and Their Applications in Environmental Water Samples and Living Cells for ClO− Detection and Imaging |
| title_sort | preparation of one emission nitrogen fluorine doped carbon quantum dots and their applications in environmental water samples and living cells for clo detection and imaging |
| url | http://dx.doi.org/10.1155/2023/7515979 |
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