Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan
Introduction: The multidrug-resistant (MDR) Salmonella enterica serovar Typhi isolates have been increasingly reported from the Asian and African countries. The emergence of isolates with decreased susceptibility to fluoroquinolones and cephalosporins has worsened the situation. Recently, an outbre...
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The Journal of Infection in Developing Countries
2020-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12049 |
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| author | Muhammad Saeed Muhammad Hidayat Rasool Farhan Rasheed Muhammad Saqalein Muhammad Atif Nisar Ambreen Anwar Imran Saba Tariq Afreenish Amir Aamer Ikram Mohsin Khurshid |
| author_facet | Muhammad Saeed Muhammad Hidayat Rasool Farhan Rasheed Muhammad Saqalein Muhammad Atif Nisar Ambreen Anwar Imran Saba Tariq Afreenish Amir Aamer Ikram Mohsin Khurshid |
| author_sort | Muhammad Saeed |
| collection | DOAJ |
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Introduction: The multidrug-resistant (MDR) Salmonella enterica serovar Typhi isolates have been increasingly reported from the Asian and African countries. The emergence of isolates with decreased susceptibility to fluoroquinolones and cephalosporins has worsened the situation. Recently, an outbreak from Sindh, Pakistan was reported caused by extensively drug-resistant (XDR) S. Typhi strains.
Methodology: In the present study, a total of 82 cases of typhoid have been investigated during 2018 from the febrile children referred to a tertiary care hospital in the population-wise largest province (Punjab) of Pakistan. S. Typhi was identified by standard microbiological techniques and isolates were characterized for antimicrobial resistance profiling and minimum inhibitory concentrations were determined. The presence of various ESBL genes in S. Typhi was confirmed by the PCR.
Results: Out of the 82 isolates tested, 35 (43%) were found to be XDR; resistant to the first-line drugs. The resistance to third-generation cephalosporins was mainly mediated by extended-spectrum beta-lactamases i.e. blaTEM and blaCTX-M genes.
Conclusions: The higher prevalence of ESBL producing Salmonella typhi clinical strains raises the concern about transmission prevention and infection management in the community as well as clinical settings. Moreover, the study highlights the problem concerning the declining antibiotic arsenal for the therapeutic management of typhoid fever and the emergence and spread of XDR strains in Pakistan.
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| format | Article |
| id | doaj-art-9acd113016464374b7d59e52c63e9d44 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2020-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-9acd113016464374b7d59e52c63e9d442025-08-20T03:52:39ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-02-01140210.3855/jidc.12049Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, PakistanMuhammad Saeed0Muhammad Hidayat Rasool1Farhan Rasheed2Muhammad Saqalein3Muhammad Atif Nisar4Ambreen Anwar Imran5Saba Tariq6Afreenish Amir7Aamer Ikram8Mohsin Khurshid9Department of Microbiology, Government College University Faisalabad, PakistanDepartment of Microbiology, Government College University Faisalabad, PakistanDepartment of Pathology, Allama Iqbal Medical College, Lahore, PakistanDepartment of Microbiology, Government College University Faisalabad, PakistanDepartment of Microbiology, Government College University Faisalabad, PakistanDepartment of Pathology, Allama Iqbal Medical College, Lahore, PakistanCentre for Applied Molecular Biology, University of the Punjab, Lahore, PakistanNational Institute of Health, Islamabad, PakistanNational Institute of Health, Islamabad, PakistanDepartment of Microbiology, Government College University Faisalabad, Pakistan Introduction: The multidrug-resistant (MDR) Salmonella enterica serovar Typhi isolates have been increasingly reported from the Asian and African countries. The emergence of isolates with decreased susceptibility to fluoroquinolones and cephalosporins has worsened the situation. Recently, an outbreak from Sindh, Pakistan was reported caused by extensively drug-resistant (XDR) S. Typhi strains. Methodology: In the present study, a total of 82 cases of typhoid have been investigated during 2018 from the febrile children referred to a tertiary care hospital in the population-wise largest province (Punjab) of Pakistan. S. Typhi was identified by standard microbiological techniques and isolates were characterized for antimicrobial resistance profiling and minimum inhibitory concentrations were determined. The presence of various ESBL genes in S. Typhi was confirmed by the PCR. Results: Out of the 82 isolates tested, 35 (43%) were found to be XDR; resistant to the first-line drugs. The resistance to third-generation cephalosporins was mainly mediated by extended-spectrum beta-lactamases i.e. blaTEM and blaCTX-M genes. Conclusions: The higher prevalence of ESBL producing Salmonella typhi clinical strains raises the concern about transmission prevention and infection management in the community as well as clinical settings. Moreover, the study highlights the problem concerning the declining antibiotic arsenal for the therapeutic management of typhoid fever and the emergence and spread of XDR strains in Pakistan. https://jidc.org/index.php/journal/article/view/12049SalmonellacephalosporinsESBLTyphoid |
| spellingShingle | Muhammad Saeed Muhammad Hidayat Rasool Farhan Rasheed Muhammad Saqalein Muhammad Atif Nisar Ambreen Anwar Imran Saba Tariq Afreenish Amir Aamer Ikram Mohsin Khurshid Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan Journal of Infection in Developing Countries Salmonella cephalosporins ESBL Typhoid |
| title | Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan |
| title_full | Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan |
| title_fullStr | Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan |
| title_full_unstemmed | Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan |
| title_short | Extended-spectrum beta-lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan |
| title_sort | extended spectrum beta lactamases producing extensively drug resistant salmonella typhi in punjab pakistan |
| topic | Salmonella cephalosporins ESBL Typhoid |
| url | https://jidc.org/index.php/journal/article/view/12049 |
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