Dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryos
Dipropyl phthalate (DPRP), a plasticizer commonly utilized in the plastics industry, has been identified in food and the environment and has the potential to present a hazard to human health and the environment. In this study, the first comprehensive evaluation of DPRP-induced craniofacial chondroge...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016798 |
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| author | Qinyuan Shen Weitao Hu Fasheng Liu Si Dong Xinya Peng Yihang Zhong Chao Chen Yuhua Zuo Chenkai Ge Weirong Li Wenwen Zha Zhijun Ye Zigang Cao Lan Liao |
| author_facet | Qinyuan Shen Weitao Hu Fasheng Liu Si Dong Xinya Peng Yihang Zhong Chao Chen Yuhua Zuo Chenkai Ge Weirong Li Wenwen Zha Zhijun Ye Zigang Cao Lan Liao |
| author_sort | Qinyuan Shen |
| collection | DOAJ |
| description | Dipropyl phthalate (DPRP), a plasticizer commonly utilized in the plastics industry, has been identified in food and the environment and has the potential to present a hazard to human health and the environment. In this study, the first comprehensive evaluation of DPRP-induced craniofacial chondrogenic defects was conducted using a zebrafish model. Zebrafish embryos were exposed to 1, 2, and 4 mg/L DPRP from 6 to 96 h post-fertilization. At 80 hpf, it was observed that exposure to DPRP resulted in craniofacial developmental malformations, which were mainly characterized by the shortening of the mandibular pharyngeal arches and the inability of the accompanying artery to elongate forward. The resulting phenotype was similar to that of micrognathia syndrome. Transcriptome sequencing and molecular docking analyses revealed that DPRP down-regulated chondrocyte-related genes and induced activation of the FoxO signaling pathway, which in turn interfered with cell proliferation and apoptosis. In this process, DPRP induced elevated levels of oxidative stress in the craniofacial pharyngeal arch while promoting inflammatory responses. This ultimately led to craniofacial chondrogenic malformations in zebrafish. The present study demonstrates that DPRP induces developmental toxicity of zebrafish craniofacial cartilage, which may have adverse effects on other aquatic organisms and humans. |
| format | Article |
| id | doaj-art-9abe70ee6fca46178c0c1a5da51149b0 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-9abe70ee6fca46178c0c1a5da51149b02025-02-12T05:29:57ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117603Dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryosQinyuan Shen0Weitao Hu1Fasheng Liu2Si Dong3Xinya Peng4Yihang Zhong5Chao Chen6Yuhua Zuo7Chenkai Ge8Weirong Li9Wenwen Zha10Zhijun Ye11Zigang Cao12Lan Liao13The Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University, Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases, Nanchang, Jiangxi 330006, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR ChinaJiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR China; Correspondence to: College of Life Sciences, Jinggangshan University, No. 28 Xueyuan Road, Qingyuan District, Ji’an, Jiangxi 343009, PR China.The Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University, Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases, Nanchang, Jiangxi 330006, PR China; Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Jiangxi Key Laboratory of Developmental Biology of Organs and Epigenetics, Clinical Research Center of Affiliated Hospital of Jinggangshan University, College of Life Sciences, Jinggangshan University, Ji’an, Jiangxi 343009, PR China; The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330006, PR China; Correspondence to: The Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases, Nanchang, Jiangxi 330006, PR China.Dipropyl phthalate (DPRP), a plasticizer commonly utilized in the plastics industry, has been identified in food and the environment and has the potential to present a hazard to human health and the environment. In this study, the first comprehensive evaluation of DPRP-induced craniofacial chondrogenic defects was conducted using a zebrafish model. Zebrafish embryos were exposed to 1, 2, and 4 mg/L DPRP from 6 to 96 h post-fertilization. At 80 hpf, it was observed that exposure to DPRP resulted in craniofacial developmental malformations, which were mainly characterized by the shortening of the mandibular pharyngeal arches and the inability of the accompanying artery to elongate forward. The resulting phenotype was similar to that of micrognathia syndrome. Transcriptome sequencing and molecular docking analyses revealed that DPRP down-regulated chondrocyte-related genes and induced activation of the FoxO signaling pathway, which in turn interfered with cell proliferation and apoptosis. In this process, DPRP induced elevated levels of oxidative stress in the craniofacial pharyngeal arch while promoting inflammatory responses. This ultimately led to craniofacial chondrogenic malformations in zebrafish. The present study demonstrates that DPRP induces developmental toxicity of zebrafish craniofacial cartilage, which may have adverse effects on other aquatic organisms and humans.http://www.sciencedirect.com/science/article/pii/S0147651324016798Dipropyl phthalateChondrogenesisZebrafishOxidative stressFoxO signaling pathway |
| spellingShingle | Qinyuan Shen Weitao Hu Fasheng Liu Si Dong Xinya Peng Yihang Zhong Chao Chen Yuhua Zuo Chenkai Ge Weirong Li Wenwen Zha Zhijun Ye Zigang Cao Lan Liao Dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryos Ecotoxicology and Environmental Safety Dipropyl phthalate Chondrogenesis Zebrafish Oxidative stress FoxO signaling pathway |
| title | Dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryos |
| title_full | Dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryos |
| title_fullStr | Dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryos |
| title_full_unstemmed | Dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryos |
| title_short | Dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryos |
| title_sort | dipropyl phthalate induces craniofacial chondrogenic defects in zebrafish embryos |
| topic | Dipropyl phthalate Chondrogenesis Zebrafish Oxidative stress FoxO signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016798 |
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