Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designs
A study on green analytical chemistry aims to develop eco-friendly alternatives to hazardous substances and minimize waste generation. The study thoroughly examined various tools to determine their greenness. A newly validated RP-UPLC method was then employed quantitatively to detect Triamcinolone A...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Green Chemistry Letters and Reviews |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17518253.2023.2301315 |
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| author | Hossam M. Nassef Hoda A. Ahmed Mohamed A. El-Atawy Tahani Y. A. Alanazi Mahmoud A. Mohamed |
| author_facet | Hossam M. Nassef Hoda A. Ahmed Mohamed A. El-Atawy Tahani Y. A. Alanazi Mahmoud A. Mohamed |
| author_sort | Hossam M. Nassef |
| collection | DOAJ |
| description | A study on green analytical chemistry aims to develop eco-friendly alternatives to hazardous substances and minimize waste generation. The study thoroughly examined various tools to determine their greenness. A newly validated RP-UPLC method was then employed quantitatively to detect Triamcinolone Acetonide (TRA) in a creamy pharmaceutical formulation using UPLC BEH C18 (50 × 2.1 mm, 1.7μm) column for analysis, with a detection wavelength set at 240 nm. The Box-Behnken design was developed to efficiently determine the optimal chromatographic conditions while minimizing the required trials. The study effectively assessed the impact of three independent variables on various responses, including retention time, tailing factor, and theoretical plates. The variables examined ethanol ratio, pH levels, and flow rate were meticulously tested to optimize experimental conditions. The utilization of desirability and overlay plots, along with a mobile phase of ethanol and purified water in a volumetric ratio of 30:70 and pH adjustment to 5.0. Calibration curves were constructed to assess the linearity of TRA within the concentration range of 2.5-50 µg/mL, yielding correlation coefficients (r = 0.9999). The accuracy was validated through recovery studies with the acceptable range of 99.6-101.2%. The specificity of the method has been validated by conducting forced degradation studies per ICH guidelines. |
| format | Article |
| id | doaj-art-9abbd8c0a3d742aebbfc6df3a6e1c222 |
| institution | OA Journals |
| issn | 1751-8253 1751-7192 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Green Chemistry Letters and Reviews |
| spelling | doaj-art-9abbd8c0a3d742aebbfc6df3a6e1c2222025-08-20T02:36:09ZengTaylor & Francis GroupGreen Chemistry Letters and Reviews1751-82531751-71922024-12-0117110.1080/17518253.2023.2301315Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designsHossam M. Nassef0Hoda A. Ahmed1Mohamed A. El-Atawy2Tahani Y. A. Alanazi3Mahmoud A. Mohamed4Chemistry Department, Faculty of Science at Yanbu, Taibah University, Yanbu, Saudi ArabiaChemistry Department, Faculty of Science at Yanbu, Taibah University, Yanbu, Saudi ArabiaChemistry Department, Faculty of Science at Yanbu, Taibah University, Yanbu, Saudi ArabiaChemistry Department, Faculty of Science, University of Ha’il, Ha’il, Saudi ArabiaHikma Pharmaceutical Company, Beni-Suef, EgyptA study on green analytical chemistry aims to develop eco-friendly alternatives to hazardous substances and minimize waste generation. The study thoroughly examined various tools to determine their greenness. A newly validated RP-UPLC method was then employed quantitatively to detect Triamcinolone Acetonide (TRA) in a creamy pharmaceutical formulation using UPLC BEH C18 (50 × 2.1 mm, 1.7μm) column for analysis, with a detection wavelength set at 240 nm. The Box-Behnken design was developed to efficiently determine the optimal chromatographic conditions while minimizing the required trials. The study effectively assessed the impact of three independent variables on various responses, including retention time, tailing factor, and theoretical plates. The variables examined ethanol ratio, pH levels, and flow rate were meticulously tested to optimize experimental conditions. The utilization of desirability and overlay plots, along with a mobile phase of ethanol and purified water in a volumetric ratio of 30:70 and pH adjustment to 5.0. Calibration curves were constructed to assess the linearity of TRA within the concentration range of 2.5-50 µg/mL, yielding correlation coefficients (r = 0.9999). The accuracy was validated through recovery studies with the acceptable range of 99.6-101.2%. The specificity of the method has been validated by conducting forced degradation studies per ICH guidelines.https://www.tandfonline.com/doi/10.1080/17518253.2023.2301315Greens assessmentRP-UPLCspecificityBox-Behnken designSix Sigma approach |
| spellingShingle | Hossam M. Nassef Hoda A. Ahmed Mohamed A. El-Atawy Tahani Y. A. Alanazi Mahmoud A. Mohamed Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designs Green Chemistry Letters and Reviews Greens assessment RP-UPLC specificity Box-Behnken design Six Sigma approach |
| title | Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designs |
| title_full | Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designs |
| title_fullStr | Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designs |
| title_full_unstemmed | Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designs |
| title_short | Greens assessment of RP-UPLC method for estimating Triamcinolone Acetonide and its degraded products compared to Box-Behnken and Six Sigma designs |
| title_sort | greens assessment of rp uplc method for estimating triamcinolone acetonide and its degraded products compared to box behnken and six sigma designs |
| topic | Greens assessment RP-UPLC specificity Box-Behnken design Six Sigma approach |
| url | https://www.tandfonline.com/doi/10.1080/17518253.2023.2301315 |
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