Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains

Abstract The fellowship, entitled ‘Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains’, was implemented at the Univers...

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Main Authors: Abdul Muhammad Ehtesham, Virginia Filipello, Pablo S. Fernandèz Escàmez, Alberto Garre Perez
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:EFSA Journal
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Online Access:https://doi.org/10.2903/j.efsa.2024.e221103
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author Abdul Muhammad Ehtesham
Virginia Filipello
Pablo S. Fernandèz Escàmez
Alberto Garre Perez
author_facet Abdul Muhammad Ehtesham
Virginia Filipello
Pablo S. Fernandèz Escàmez
Alberto Garre Perez
author_sort Abdul Muhammad Ehtesham
collection DOAJ
description Abstract The fellowship, entitled ‘Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains’, was implemented at the Universidad Politécnica de Cartagena (UPCT), Spain. Supervised by Dr. Alberto Garre and Prof. Pablo S. Fernandez and coordinated by Dr. Virginia Filipello of the Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Italy, the fellowship aimed to provide hands‐on training in quantitative microbial risk assessment (QMRA). The fellow benefited from UPCT's expertise in microbiological risk assessment, gaining knowledge of methodologies, terminologies and software tools essential for QMRA. The focus of the fellowship was on the risks associated with plant‐based milk products, which are increasingly popular as sustainable alternatives to dairy milk. Despite the heat treatments these beverages undergo to ensure safety, risks persist, such as cross‐contamination during post‐processing or the survival of heat‐resistant spores like Bacillus cereus. A recent European outbreak linked to contaminated oat milk underscored the importance of assessing these risks. The project was conducted in two phases: first, at UPCT's Food Microbiology Laboratory, where the fellow handled and characterised the thermal resistance of various B. cereus strains using a thermoresistometer; and second, through remote analysis of experimental data using risk analysis software tools. The fellow developed skills in microbiological techniques, such as spore preparation and thermal resistance evaluation, and became proficient in data analysis using the R programming language and the biorisk package. The fellowship culminated in the development of a QMRA model to estimate the likelihood of B. cereus‐related foodborne illness from plant‐based milks, considering different heat treatments and bacterial strains. The fellow's training covered all stages of risk assessment, including hazard identification, exposure assessment, hazard characterisation and risk characterisation, providing a comprehensive foundation for a career in food safety and microbial risk assessment.
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spelling doaj-art-9abbb8f0b33641d8929b8a4e478b03ed2025-08-20T02:39:11ZengWileyEFSA Journal1831-47322024-12-0122S1n/an/a10.2903/j.efsa.2024.e221103Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chainsAbdul Muhammad Ehtesham0Virginia Filipello1Pablo S. Fernandèz Escàmez2Alberto Garre Perez3Istituto zooprofilattico della Lombardia e dell'Emilia RomagnaIstituto zooprofilattico della Lombardia e dell'Emilia RomagnaDepartemento de Ingenierìa Agronòmica ETSIA‐ Universidad politècnica de Cartagena Murcia SpainDepartemento de Ingenierìa Agronòmica ETSIA‐ Universidad politècnica de Cartagena Murcia SpainAbstract The fellowship, entitled ‘Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains’, was implemented at the Universidad Politécnica de Cartagena (UPCT), Spain. Supervised by Dr. Alberto Garre and Prof. Pablo S. Fernandez and coordinated by Dr. Virginia Filipello of the Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Italy, the fellowship aimed to provide hands‐on training in quantitative microbial risk assessment (QMRA). The fellow benefited from UPCT's expertise in microbiological risk assessment, gaining knowledge of methodologies, terminologies and software tools essential for QMRA. The focus of the fellowship was on the risks associated with plant‐based milk products, which are increasingly popular as sustainable alternatives to dairy milk. Despite the heat treatments these beverages undergo to ensure safety, risks persist, such as cross‐contamination during post‐processing or the survival of heat‐resistant spores like Bacillus cereus. A recent European outbreak linked to contaminated oat milk underscored the importance of assessing these risks. The project was conducted in two phases: first, at UPCT's Food Microbiology Laboratory, where the fellow handled and characterised the thermal resistance of various B. cereus strains using a thermoresistometer; and second, through remote analysis of experimental data using risk analysis software tools. The fellow developed skills in microbiological techniques, such as spore preparation and thermal resistance evaluation, and became proficient in data analysis using the R programming language and the biorisk package. The fellowship culminated in the development of a QMRA model to estimate the likelihood of B. cereus‐related foodborne illness from plant‐based milks, considering different heat treatments and bacterial strains. The fellow's training covered all stages of risk assessment, including hazard identification, exposure assessment, hazard characterisation and risk characterisation, providing a comprehensive foundation for a career in food safety and microbial risk assessment.https://doi.org/10.2903/j.efsa.2024.e221103bacillus cereusplant‐based milkquantitative microbiological risk assessment
spellingShingle Abdul Muhammad Ehtesham
Virginia Filipello
Pablo S. Fernandèz Escàmez
Alberto Garre Perez
Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains
EFSA Journal
bacillus cereus
plant‐based milk
quantitative microbiological risk assessment
title Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains
title_full Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains
title_fullStr Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains
title_full_unstemmed Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains
title_short Training in modern statistical methodologies and software tools for the definition and analysis of (stochastic) quantitative microbial risk assessment models with relevant food products for the Italian and Spanish food supply chains
title_sort training in modern statistical methodologies and software tools for the definition and analysis of stochastic quantitative microbial risk assessment models with relevant food products for the italian and spanish food supply chains
topic bacillus cereus
plant‐based milk
quantitative microbiological risk assessment
url https://doi.org/10.2903/j.efsa.2024.e221103
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