Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection
Abstract The biofilm acts as a protective layer for Streptococcus suis (S. suis), contributing to the development of drug resistance and chronic infections. Autoinducer 2 (AI-2) quorum sensing represents the primary regulatory pathway governing biofilm formation in S. suis. Consequently, targeting A...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-025-01450-x |
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| author | Shuji Gao Shuo Yuan Yingying Quan Wenjie Jin Yamin Shen Rishun Li Baobao Liu Yuxin Wang Li Yi Shaohui Wang Xiaogai Hou Yang Wang |
| author_facet | Shuji Gao Shuo Yuan Yingying Quan Wenjie Jin Yamin Shen Rishun Li Baobao Liu Yuxin Wang Li Yi Shaohui Wang Xiaogai Hou Yang Wang |
| author_sort | Shuji Gao |
| collection | DOAJ |
| description | Abstract The biofilm acts as a protective layer for Streptococcus suis (S. suis), contributing to the development of drug resistance and chronic infections. Autoinducer 2 (AI-2) quorum sensing represents the primary regulatory pathway governing biofilm formation in S. suis. Consequently, targeting AI-2 quorum sensing to inhibit biofilm formation represents a promising strategy for preventing and managing drug resistance and chronic infections caused by S. suis. This study established a small natural product library by integrating commercial drug molecules with Chinese herbal medicine molecules. Consequently, two natural products, salvianolic acid A (SAA) and rhapontin (RH), which target S. suis AI-2 via quorum sensing, were identified. SAA and RH inhibit AI-2 synthesis through noncompetitive and competitive binding to S-ribosylhomocysteinase (LuxS). By inhibiting S. suis AI-2 quorum sensing, these compounds modulate the expression of adhesion genes and the synthesis of extracellular polysaccharides (EPS), reducing the adhesion ability of S. suis and ultimately inhibiting biofilm formation. Using LC‒MS/MS, we further analysed the impact of SAA and RH on the metabolic activity of S. suis, revealing the potential medicinal value of these compounds. Finally, the efficacy of SAA and RH against S. suis infection was validated in Galleria mellonella larvae, confirming their significant anti-infection effects. |
| format | Article |
| id | doaj-art-9abaf7df9b3b46d1843eb7c634c09c13 |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-9abaf7df9b3b46d1843eb7c634c09c132025-02-09T12:50:05ZengBMCVeterinary Research1297-97162025-02-0156111910.1186/s13567-025-01450-xTargeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infectionShuji Gao0Shuo Yuan1Yingying Quan2Wenjie Jin3Yamin Shen4Rishun Li5Baobao Liu6Yuxin Wang7Li Yi8Shaohui Wang9Xiaogai Hou10Yang Wang11College of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyHenan Provincial Engineering Research Center for Detection and Prevention and Control of Emerging Infectious Diseases in Livestock and PoultryCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Agriculture/College of Tree Peony, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyAbstract The biofilm acts as a protective layer for Streptococcus suis (S. suis), contributing to the development of drug resistance and chronic infections. Autoinducer 2 (AI-2) quorum sensing represents the primary regulatory pathway governing biofilm formation in S. suis. Consequently, targeting AI-2 quorum sensing to inhibit biofilm formation represents a promising strategy for preventing and managing drug resistance and chronic infections caused by S. suis. This study established a small natural product library by integrating commercial drug molecules with Chinese herbal medicine molecules. Consequently, two natural products, salvianolic acid A (SAA) and rhapontin (RH), which target S. suis AI-2 via quorum sensing, were identified. SAA and RH inhibit AI-2 synthesis through noncompetitive and competitive binding to S-ribosylhomocysteinase (LuxS). By inhibiting S. suis AI-2 quorum sensing, these compounds modulate the expression of adhesion genes and the synthesis of extracellular polysaccharides (EPS), reducing the adhesion ability of S. suis and ultimately inhibiting biofilm formation. Using LC‒MS/MS, we further analysed the impact of SAA and RH on the metabolic activity of S. suis, revealing the potential medicinal value of these compounds. Finally, the efficacy of SAA and RH against S. suis infection was validated in Galleria mellonella larvae, confirming their significant anti-infection effects.https://doi.org/10.1186/s13567-025-01450-xStreptococcus suisbiofilmnatural productsquorum sensingvirulence |
| spellingShingle | Shuji Gao Shuo Yuan Yingying Quan Wenjie Jin Yamin Shen Rishun Li Baobao Liu Yuxin Wang Li Yi Shaohui Wang Xiaogai Hou Yang Wang Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection Veterinary Research Streptococcus suis biofilm natural products quorum sensing virulence |
| title | Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection |
| title_full | Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection |
| title_fullStr | Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection |
| title_full_unstemmed | Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection |
| title_short | Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection |
| title_sort | targeting ai 2 quorum sensing harnessing natural products against streptococcus suis biofilm infection |
| topic | Streptococcus suis biofilm natural products quorum sensing virulence |
| url | https://doi.org/10.1186/s13567-025-01450-x |
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