A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic Diversity

This Systematic Literature Review (SLR) consolidates current research on mitochondrial DNA (mtDNA) analysis in horses, focusing on genetic variation, maternal lineage tracing, and haplogroup identification. The article selection process screened 1380 articles, with 76 fulfilling the inclusion criter...

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Main Authors: Ayman Agbani, Oumaima Aminou, Mohamed Machmoum, Agnes Germot, Bouabid Badaoui, Daniel Petit, Mohammed Piro
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/6/885
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author Ayman Agbani
Oumaima Aminou
Mohamed Machmoum
Agnes Germot
Bouabid Badaoui
Daniel Petit
Mohammed Piro
author_facet Ayman Agbani
Oumaima Aminou
Mohamed Machmoum
Agnes Germot
Bouabid Badaoui
Daniel Petit
Mohammed Piro
author_sort Ayman Agbani
collection DOAJ
description This Systematic Literature Review (SLR) consolidates current research on mitochondrial DNA (mtDNA) analysis in horses, focusing on genetic variation, maternal lineage tracing, and haplogroup identification. The article selection process screened 1380 articles, with 76 fulfilling the inclusion criteria. Data extraction covered sampling techniques, studied mtDNA regions, sequencing methods, and haplogroup identification. Following the methodology of the PRISMA guidelines, this review encompasses studies published since 2012, obtained from Scopus, PubMed, Research4Life, Web of Science, and ScienceDirect. The major findings emphasise the use of mtDNA for tracing ancestry, validating maternal lineages, and identifying haplogroups along with their geographic distributions. This review identifies challenges, including the need to update the haplogroup classification system and potential information loss due to sequence trimming. Additionally, it examines promising avenues for future research, such as the implementation of next-generation sequencing and the merging of haplogroup data with performance traits, which could influence conservation initiatives and breeding programs. This review emphasises the necessity for standardised classification systems and further research on underrepresented breeds and regions to improve our understanding of equine genetic diversity.
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series Animals
spelling doaj-art-9ab930c9a76646148ee744b2bd0083cc2025-08-20T03:43:50ZengMDPI AGAnimals2076-26152025-03-0115688510.3390/ani15060885A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic DiversityAyman Agbani0Oumaima Aminou1Mohamed Machmoum2Agnes Germot3Bouabid Badaoui4Daniel Petit5Mohammed Piro6Laboratory of Veterinary Genetic Analysis, Department of Medicine, Surgery and Reproduction Agronomic, Veterinary Institute Hassan II Rabat, Rabat B.P. 6202, MoroccoLaboratory of Veterinary Genetic Analysis, Department of Medicine, Surgery and Reproduction Agronomic, Veterinary Institute Hassan II Rabat, Rabat B.P. 6202, MoroccoLaboratory of Veterinary Genetic Analysis, Department of Medicine, Surgery and Reproduction Agronomic, Veterinary Institute Hassan II Rabat, Rabat B.P. 6202, MoroccoLABCiS, UR 22722, Faculty of Sciences and Technology, University of Limoges, F-87000 Limoges, FranceDepartment of Biology, Faculty of Science, Mohammed V University, Rabat B.P. 8007, MoroccoLABCiS, UR 22722, Faculty of Sciences and Technology, University of Limoges, F-87000 Limoges, FranceLaboratory of Veterinary Genetic Analysis, Department of Medicine, Surgery and Reproduction Agronomic, Veterinary Institute Hassan II Rabat, Rabat B.P. 6202, MoroccoThis Systematic Literature Review (SLR) consolidates current research on mitochondrial DNA (mtDNA) analysis in horses, focusing on genetic variation, maternal lineage tracing, and haplogroup identification. The article selection process screened 1380 articles, with 76 fulfilling the inclusion criteria. Data extraction covered sampling techniques, studied mtDNA regions, sequencing methods, and haplogroup identification. Following the methodology of the PRISMA guidelines, this review encompasses studies published since 2012, obtained from Scopus, PubMed, Research4Life, Web of Science, and ScienceDirect. The major findings emphasise the use of mtDNA for tracing ancestry, validating maternal lineages, and identifying haplogroups along with their geographic distributions. This review identifies challenges, including the need to update the haplogroup classification system and potential information loss due to sequence trimming. Additionally, it examines promising avenues for future research, such as the implementation of next-generation sequencing and the merging of haplogroup data with performance traits, which could influence conservation initiatives and breeding programs. This review emphasises the necessity for standardised classification systems and further research on underrepresented breeds and regions to improve our understanding of equine genetic diversity.https://www.mdpi.com/2076-2615/15/6/885mitochondrial DNAequine maternal lineageshorse genetic diversitymtDNA haplogroupsphylogenypopulation genetics
spellingShingle Ayman Agbani
Oumaima Aminou
Mohamed Machmoum
Agnes Germot
Bouabid Badaoui
Daniel Petit
Mohammed Piro
A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic Diversity
Animals
mitochondrial DNA
equine maternal lineages
horse genetic diversity
mtDNA haplogroups
phylogeny
population genetics
title A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic Diversity
title_full A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic Diversity
title_fullStr A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic Diversity
title_full_unstemmed A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic Diversity
title_short A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic Diversity
title_sort systematic literature review of mitochondrial dna analysis for horse genetic diversity
topic mitochondrial DNA
equine maternal lineages
horse genetic diversity
mtDNA haplogroups
phylogeny
population genetics
url https://www.mdpi.com/2076-2615/15/6/885
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