Blastocystis sp. of Subtypes 2, 4 and 9 in Selected Avian Species, Brazil, 2009-2018
ABSTRACT Avian species of the metropolitan region of Belo Horizonte, Brazil, submitted to laboratory routine due to a variety of morbid conditions, were diagnosed as carriers of Blastocystis sp. All samples were examined under light microscopy and those with structures suggestive of Blastocystis sp....
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Fundação APINCO de Ciência e Tecnologia Avícolas
2024-11-01
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| Series: | Brazilian Journal of Poultry Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2024000300305&lng=en&tlng=en |
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| author | SYG Marin CR Silva JP Andrade CF Soares PHC Rodrigues HCAM Oliveira DAR Vilela HLG Coelho OC Freitas Neto NRS Martins |
| author_facet | SYG Marin CR Silva JP Andrade CF Soares PHC Rodrigues HCAM Oliveira DAR Vilela HLG Coelho OC Freitas Neto NRS Martins |
| author_sort | SYG Marin |
| collection | DOAJ |
| description | ABSTRACT Avian species of the metropolitan region of Belo Horizonte, Brazil, submitted to laboratory routine due to a variety of morbid conditions, were diagnosed as carriers of Blastocystis sp. All samples were examined under light microscopy and those with structures suggestive of Blastocystis sp. were tested by PCR. The total DNA was purified from feces and/or intestinal mucosa and used as a template for PCR, targeting the small subunit (SSU) rRNA gene, and the products were sequenced. The sequences detected in two chickens, one passerine (Saltator similis, Thraupidae) and a golden parakeet (Guaruba guarouba, Psittacidae) were phylogenetically characterized and shown to cluster with sequences of subtypes 2, 4 or 9. The occurrence of Blastocystis sp. in domestic and local native avifauna is described and the perspective of a complex local interspecies epidemiology is considered of risk, including to humans. |
| format | Article |
| id | doaj-art-9ab7fee9b2be4a9cbf9af84e2edbd747 |
| institution | Kabale University |
| issn | 1806-9061 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj-art-9ab7fee9b2be4a9cbf9af84e2edbd7472024-11-12T07:45:13ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612024-11-0126310.1590/1806-9061-2023-1836Blastocystis sp. of Subtypes 2, 4 and 9 in Selected Avian Species, Brazil, 2009-2018SYG Marinhttps://orcid.org/0000-0002-9374-5591CR Silvahttps://orcid.org/0009-0001-3753-1708JP Andradehttps://orcid.org/0000-0003-2108-9748CF Soareshttps://orcid.org/0009-0002-2837-7603PHC Rodrigueshttps://orcid.org/0009-0009-2672-609XHCAM Oliveirahttps://orcid.org/0009-0009-9580-782XDAR Vilelahttps://orcid.org/0000-0001-5707-3233HLG Coelhohttps://orcid.org/0000-0003-1460-0202OC Freitas Netohttps://orcid.org/0000-0002-1437-069XNRS Martinshttps://orcid.org/0000-0001-8925-2228ABSTRACT Avian species of the metropolitan region of Belo Horizonte, Brazil, submitted to laboratory routine due to a variety of morbid conditions, were diagnosed as carriers of Blastocystis sp. All samples were examined under light microscopy and those with structures suggestive of Blastocystis sp. were tested by PCR. The total DNA was purified from feces and/or intestinal mucosa and used as a template for PCR, targeting the small subunit (SSU) rRNA gene, and the products were sequenced. The sequences detected in two chickens, one passerine (Saltator similis, Thraupidae) and a golden parakeet (Guaruba guarouba, Psittacidae) were phylogenetically characterized and shown to cluster with sequences of subtypes 2, 4 or 9. The occurrence of Blastocystis sp. in domestic and local native avifauna is described and the perspective of a complex local interspecies epidemiology is considered of risk, including to humans.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2024000300305&lng=en&tlng=enStramenopilesGallus gallus domesticusGuaruba guaroubaSaltator similisSicalis flaveola |
| spellingShingle | SYG Marin CR Silva JP Andrade CF Soares PHC Rodrigues HCAM Oliveira DAR Vilela HLG Coelho OC Freitas Neto NRS Martins Blastocystis sp. of Subtypes 2, 4 and 9 in Selected Avian Species, Brazil, 2009-2018 Brazilian Journal of Poultry Science Stramenopiles Gallus gallus domesticus Guaruba guarouba Saltator similis Sicalis flaveola |
| title | Blastocystis sp. of Subtypes 2, 4 and 9 in Selected Avian Species, Brazil, 2009-2018 |
| title_full | Blastocystis sp. of Subtypes 2, 4 and 9 in Selected Avian Species, Brazil, 2009-2018 |
| title_fullStr | Blastocystis sp. of Subtypes 2, 4 and 9 in Selected Avian Species, Brazil, 2009-2018 |
| title_full_unstemmed | Blastocystis sp. of Subtypes 2, 4 and 9 in Selected Avian Species, Brazil, 2009-2018 |
| title_short | Blastocystis sp. of Subtypes 2, 4 and 9 in Selected Avian Species, Brazil, 2009-2018 |
| title_sort | blastocystis sp of subtypes 2 4 and 9 in selected avian species brazil 2009 2018 |
| topic | Stramenopiles Gallus gallus domesticus Guaruba guarouba Saltator similis Sicalis flaveola |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2024000300305&lng=en&tlng=en |
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