Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection
This study includes isolation of 25 isolates of Escherichia coli (E. coli ) strain from urinary tract samples in a pregnant woman. Microbiological and biochemical tests were used to identify the resistant bacteria of this genus. Screening methods were used to determine bacterial isolates for their...
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| Format: | Article |
| Language: | English |
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Sulaimani Polytechnic University
2018-10-01
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| Series: | Kurdistan Journal of Applied Research |
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| Online Access: | https://kjar.spu.edu.iq/index.php/kjar/article/view/218 |
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| author | Karzan Abdulmuhsin Mohammad Zirak F. Ahmed Bayar A Mohammed Rasti H Saeed |
| author_facet | Karzan Abdulmuhsin Mohammad Zirak F. Ahmed Bayar A Mohammed Rasti H Saeed |
| author_sort | Karzan Abdulmuhsin Mohammad |
| collection | DOAJ |
| description | This study includes isolation of 25 isolates of Escherichia coli (E. coli ) strain from urinary tract samples in a pregnant woman. Microbiological and biochemical tests were used to identify the resistant bacteria of this genus. Screening methods were used to determine bacterial isolates for their resistance to 10 antibiotics include: Amikacin (Ak), Amoxicillin (Ax), Ampicillin (Ap), Chloramphenicol (Cm), Ciprofloxacin (Cip), Erythromycin (Er), Nalidixic acid (Nal), Penicillin (Pen), Tetracycline (Tet) and Trimethoprim (Tm). The isolates E4, E9, E16, and E17 were resistant to all antibiotics used in the current study using the disk diffusion method. In contrast, the resistance percentage for all antibiotics ranged between 28-96%. Sites of resistance genes and hemolysin production genes were characterized by tranformation techniques in the E4 and E16. The results showed that the antibiotic resistance genes of Amikacin, Erythromycin, Tetracyclin, and Trimethoprim were located on a plasmid, whereas Amoxicillin, Ampicillin, Chloramphenicol, Ciprofloxacin, Nalidixic acid and Penicillin were located on chromosomal DNA. The results also demonstrated an inability to produce alpha or beta-hemolysin indicating that the genes which are responsible for hemolysin production were also located on chromosomal DNA.
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| format | Article |
| id | doaj-art-9ab3866a5d0b4164944b367dc70ffcd9 |
| institution | Kabale University |
| issn | 2411-7684 2411-7706 |
| language | English |
| publishDate | 2018-10-01 |
| publisher | Sulaimani Polytechnic University |
| record_format | Article |
| series | Kurdistan Journal of Applied Research |
| spelling | doaj-art-9ab3866a5d0b4164944b367dc70ffcd92025-02-09T21:00:43ZengSulaimani Polytechnic UniversityKurdistan Journal of Applied Research2411-76842411-77062018-10-013210.24017/science.2018.3.2218Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract InfectionKarzan Abdulmuhsin Mohammad0Zirak F. Ahmed1Bayar A Mohammed2Rasti H Saeed3Medical Analysis Department, Science Faculty | Ishik university, College of Education | Salahaddin University, Erbil, IraqBiology Department, College of Education, Salahaddin University, Erbil, IraqBiology Department, College of Education, Salahaddin University, Erbil, IraqBiology Department, College of Education, Salahaddin University, Erbil, IraqThis study includes isolation of 25 isolates of Escherichia coli (E. coli ) strain from urinary tract samples in a pregnant woman. Microbiological and biochemical tests were used to identify the resistant bacteria of this genus. Screening methods were used to determine bacterial isolates for their resistance to 10 antibiotics include: Amikacin (Ak), Amoxicillin (Ax), Ampicillin (Ap), Chloramphenicol (Cm), Ciprofloxacin (Cip), Erythromycin (Er), Nalidixic acid (Nal), Penicillin (Pen), Tetracycline (Tet) and Trimethoprim (Tm). The isolates E4, E9, E16, and E17 were resistant to all antibiotics used in the current study using the disk diffusion method. In contrast, the resistance percentage for all antibiotics ranged between 28-96%. Sites of resistance genes and hemolysin production genes were characterized by tranformation techniques in the E4 and E16. The results showed that the antibiotic resistance genes of Amikacin, Erythromycin, Tetracyclin, and Trimethoprim were located on a plasmid, whereas Amoxicillin, Ampicillin, Chloramphenicol, Ciprofloxacin, Nalidixic acid and Penicillin were located on chromosomal DNA. The results also demonstrated an inability to produce alpha or beta-hemolysin indicating that the genes which are responsible for hemolysin production were also located on chromosomal DNA. https://kjar.spu.edu.iq/index.php/kjar/article/view/218E. coli , antibiotics, resistant genes, plasmid |
| spellingShingle | Karzan Abdulmuhsin Mohammad Zirak F. Ahmed Bayar A Mohammed Rasti H Saeed Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection Kurdistan Journal of Applied Research E. coli , antibiotics, resistant genes, plasmid |
| title | Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection |
| title_full | Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection |
| title_fullStr | Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection |
| title_full_unstemmed | Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection |
| title_short | Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection |
| title_sort | determination the site of antibiotic resistance genes in escherichia coli isolated from urinary tract infection |
| topic | E. coli , antibiotics, resistant genes, plasmid |
| url | https://kjar.spu.edu.iq/index.php/kjar/article/view/218 |
| work_keys_str_mv | AT karzanabdulmuhsinmohammad determinationthesiteofantibioticresistancegenesinescherichiacoliisolatedfromurinarytractinfection AT zirakfahmed determinationthesiteofantibioticresistancegenesinescherichiacoliisolatedfromurinarytractinfection AT bayaramohammed determinationthesiteofantibioticresistancegenesinescherichiacoliisolatedfromurinarytractinfection AT rastihsaeed determinationthesiteofantibioticresistancegenesinescherichiacoliisolatedfromurinarytractinfection |