Influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti-CSPG4 electrovaccination: a retrospective study on 77 cases
The most appropriate approach to regional/sentinel lymph nodes (LN) for staging canine oral malignant melanoma (OMM) is still controversial. This study aims to retrospectively evaluate the prognostic impact of neck dissection modality and LN metastasis in a homogeneous cohort of dogs treated by surg...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-07-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1616419/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849417933427572736 |
|---|---|
| author | Davide Giacobino Matteo Olimpo Erica Ilaria Ferraris Greta Martinelli Lorella Maniscalco Mariateresa Camerino Federica Riccardo Federica Cavallo Lidia Tarone Marzia Cino Alfredo Dentini Selina Iussich Elena Lardone Luca Manassero Raffaella De Maria Paolo Buracco Emanuela Morello |
| author_facet | Davide Giacobino Matteo Olimpo Erica Ilaria Ferraris Greta Martinelli Lorella Maniscalco Mariateresa Camerino Federica Riccardo Federica Cavallo Lidia Tarone Marzia Cino Alfredo Dentini Selina Iussich Elena Lardone Luca Manassero Raffaella De Maria Paolo Buracco Emanuela Morello |
| author_sort | Davide Giacobino |
| collection | DOAJ |
| description | The most appropriate approach to regional/sentinel lymph nodes (LN) for staging canine oral malignant melanoma (OMM) is still controversial. This study aims to retrospectively evaluate the prognostic impact of neck dissection modality and LN metastasis in a homogeneous cohort of dogs treated by surgery and adjuvant anti-CSPG4 electrovaccination. Seventy-seven dogs were enrolled and divided into two groups based on the presence (Group A, 24 dogs) or absence (Group B, 53 dogs) of histologically confirmed LN metastasis at the time of surgery. The overall LN metastatic rate was 31%; metastasis was found mostly in the mandibular lymph center (83%). Median survival time (MST) and disease-free interval (DFI) in Group A were 406 and 134 days, respectively. Although shorter, these values were not significantly different from MST and DFI in Group B (534 and 219 days, respectively; p = 0.16 and p = 0.11). Stratifying the cases based on the type of lymphadenectomy performed, no statistical differences were observed between Groups 1 (ipsilateral lymphadenectomy) and 2 (bilateral lymphadenectomy) regarding both MST and DFI. Similarly, no significant differences in MST and DFI were observed among subgroups based on ipsilateral (Group 4) and bilateral (Group 6) removal versus ipsilateral (Group 3) and bilateral (Group 5) non-removal of even the medial retropharyngeal LN. No association was found between LN metastasis and recurrence or distant metastasis. Finally, no association was found between lymphadenectomy pattern and progressive disease. The results recorded in this study, i.e., that ipsilateral mandibular lymphadenectomy may be a reasonable surgical option in OMM, apply for this cohort of dogs only, and the translation of this principle to canine OMMs differently treated needs further investigations. Additionally, further efforts should be addressed to studies on sentinel LN identification for canine OMM staging. |
| format | Article |
| id | doaj-art-9aaa96c0559a4435be6ffeb89c1d9240 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-9aaa96c0559a4435be6ffeb89c1d92402025-08-20T03:32:36ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-07-011210.3389/fvets.2025.16164191616419Influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti-CSPG4 electrovaccination: a retrospective study on 77 casesDavide Giacobino0Matteo Olimpo1Erica Ilaria Ferraris2Greta Martinelli3Lorella Maniscalco4Mariateresa Camerino5Federica Riccardo6Federica Cavallo7Lidia Tarone8Marzia Cino9Alfredo Dentini10Selina Iussich11Elena Lardone12Luca Manassero13Raffaella De Maria14Paolo Buracco15Emanuela Morello16Department of Veterinary Sciences, University of Torino, Torino, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyAnimal Oncology and Imaging Center AG, Hünenberg, SwitzerlandDepartment of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, ItalyDepartment of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, ItalyDepartment of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, ItalyDepartment of Medical Veterinary Science, University of Parma, Parma, ItalyClinica Veterinaria Tyrus, Terni, ItalyMyLav La Vallonea, Veterinary Analysis Laboratory srl, Alessano, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyDepartment of Veterinary Sciences, University of Torino, Torino, ItalyThe most appropriate approach to regional/sentinel lymph nodes (LN) for staging canine oral malignant melanoma (OMM) is still controversial. This study aims to retrospectively evaluate the prognostic impact of neck dissection modality and LN metastasis in a homogeneous cohort of dogs treated by surgery and adjuvant anti-CSPG4 electrovaccination. Seventy-seven dogs were enrolled and divided into two groups based on the presence (Group A, 24 dogs) or absence (Group B, 53 dogs) of histologically confirmed LN metastasis at the time of surgery. The overall LN metastatic rate was 31%; metastasis was found mostly in the mandibular lymph center (83%). Median survival time (MST) and disease-free interval (DFI) in Group A were 406 and 134 days, respectively. Although shorter, these values were not significantly different from MST and DFI in Group B (534 and 219 days, respectively; p = 0.16 and p = 0.11). Stratifying the cases based on the type of lymphadenectomy performed, no statistical differences were observed between Groups 1 (ipsilateral lymphadenectomy) and 2 (bilateral lymphadenectomy) regarding both MST and DFI. Similarly, no significant differences in MST and DFI were observed among subgroups based on ipsilateral (Group 4) and bilateral (Group 6) removal versus ipsilateral (Group 3) and bilateral (Group 5) non-removal of even the medial retropharyngeal LN. No association was found between LN metastasis and recurrence or distant metastasis. Finally, no association was found between lymphadenectomy pattern and progressive disease. The results recorded in this study, i.e., that ipsilateral mandibular lymphadenectomy may be a reasonable surgical option in OMM, apply for this cohort of dogs only, and the translation of this principle to canine OMMs differently treated needs further investigations. Additionally, further efforts should be addressed to studies on sentinel LN identification for canine OMM staging.https://www.frontiersin.org/articles/10.3389/fvets.2025.1616419/fulllymph nodeoral malignant melanomadogCSPG4lymph node dissection |
| spellingShingle | Davide Giacobino Matteo Olimpo Erica Ilaria Ferraris Greta Martinelli Lorella Maniscalco Mariateresa Camerino Federica Riccardo Federica Cavallo Lidia Tarone Marzia Cino Alfredo Dentini Selina Iussich Elena Lardone Luca Manassero Raffaella De Maria Paolo Buracco Emanuela Morello Influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti-CSPG4 electrovaccination: a retrospective study on 77 cases Frontiers in Veterinary Science lymph node oral malignant melanoma dog CSPG4 lymph node dissection |
| title | Influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti-CSPG4 electrovaccination: a retrospective study on 77 cases |
| title_full | Influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti-CSPG4 electrovaccination: a retrospective study on 77 cases |
| title_fullStr | Influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti-CSPG4 electrovaccination: a retrospective study on 77 cases |
| title_full_unstemmed | Influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti-CSPG4 electrovaccination: a retrospective study on 77 cases |
| title_short | Influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti-CSPG4 electrovaccination: a retrospective study on 77 cases |
| title_sort | influence of the extent of cervical lymph node dissection and lymph nodes metastases on prognosis in a cohort of dogs with oral malignant melanoma treated by surgical resection and adjuvant anti cspg4 electrovaccination a retrospective study on 77 cases |
| topic | lymph node oral malignant melanoma dog CSPG4 lymph node dissection |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1616419/full |
| work_keys_str_mv | AT davidegiacobino influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT matteoolimpo influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT ericailariaferraris influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT gretamartinelli influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT lorellamaniscalco influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT mariateresacamerino influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT federicariccardo influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT federicacavallo influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT lidiatarone influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT marziacino influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT alfredodentini influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT selinaiussich influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT elenalardone influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT lucamanassero influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT raffaellademaria influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT paoloburacco influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases AT emanuelamorello influenceoftheextentofcervicallymphnodedissectionandlymphnodesmetastasesonprognosisinacohortofdogswithoralmalignantmelanomatreatedbysurgicalresectionandadjuvantanticspg4electrovaccinationaretrospectivestudyon77cases |