The Application of Single-Cell RNA Sequencing in Vaccinology
Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases an...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/8624963 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567464138899456 |
|---|---|
| author | Andrés Noé Tamsin N. Cargill Carolyn M. Nielsen Andrew J. C. Russell Eleanor Barnes |
| author_facet | Andrés Noé Tamsin N. Cargill Carolyn M. Nielsen Andrew J. C. Russell Eleanor Barnes |
| author_sort | Andrés Noé |
| collection | DOAJ |
| description | Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell RNA sequencing has been used to evaluate the immunological response to different vaccine platforms and regimens. By highlighting published and unpublished single-cell RNA sequencing studies relevant to vaccinology, we discuss some general considerations how the field could be enriched with the widespread adoption of this technology. |
| format | Article |
| id | doaj-art-9aa93c1a5b9547b5b110fc43972a2890 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9aa93c1a5b9547b5b110fc43972a28902025-02-03T01:01:28ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/86249638624963The Application of Single-Cell RNA Sequencing in VaccinologyAndrés Noé0Tamsin N. Cargill1Carolyn M. Nielsen2Andrew J. C. Russell3Eleanor Barnes4The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UKPeter Medawar Building for Pathogen Research and Oxford NIHR Biomedical Research Centre, Nuffield Department of Medicine, University of Oxford, South Parks Road, Oxford OX1 3SY, UKThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, UKWellcome Sanger Institute, Wellcome Genome Campus, Cambridge CB10 1SA, UKPeter Medawar Building for Pathogen Research and Oxford NIHR Biomedical Research Centre, Nuffield Department of Medicine, University of Oxford, South Parks Road, Oxford OX1 3SY, UKSingle-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell RNA sequencing has been used to evaluate the immunological response to different vaccine platforms and regimens. By highlighting published and unpublished single-cell RNA sequencing studies relevant to vaccinology, we discuss some general considerations how the field could be enriched with the widespread adoption of this technology.http://dx.doi.org/10.1155/2020/8624963 |
| spellingShingle | Andrés Noé Tamsin N. Cargill Carolyn M. Nielsen Andrew J. C. Russell Eleanor Barnes The Application of Single-Cell RNA Sequencing in Vaccinology Journal of Immunology Research |
| title | The Application of Single-Cell RNA Sequencing in Vaccinology |
| title_full | The Application of Single-Cell RNA Sequencing in Vaccinology |
| title_fullStr | The Application of Single-Cell RNA Sequencing in Vaccinology |
| title_full_unstemmed | The Application of Single-Cell RNA Sequencing in Vaccinology |
| title_short | The Application of Single-Cell RNA Sequencing in Vaccinology |
| title_sort | application of single cell rna sequencing in vaccinology |
| url | http://dx.doi.org/10.1155/2020/8624963 |
| work_keys_str_mv | AT andresnoe theapplicationofsinglecellrnasequencinginvaccinology AT tamsinncargill theapplicationofsinglecellrnasequencinginvaccinology AT carolynmnielsen theapplicationofsinglecellrnasequencinginvaccinology AT andrewjcrussell theapplicationofsinglecellrnasequencinginvaccinology AT eleanorbarnes theapplicationofsinglecellrnasequencinginvaccinology AT andresnoe applicationofsinglecellrnasequencinginvaccinology AT tamsinncargill applicationofsinglecellrnasequencinginvaccinology AT carolynmnielsen applicationofsinglecellrnasequencinginvaccinology AT andrewjcrussell applicationofsinglecellrnasequencinginvaccinology AT eleanorbarnes applicationofsinglecellrnasequencinginvaccinology |