Sucrose non-fermenting protein kinase gene UvSnf1 is required for virulence in Ustilaginoidea virens
Rice false smut caused by Ustilaginoidea virens is becoming one of the most devastating diseases in rice production areas in the world. Revealing U. virens potential pathogenic mechanisms provides ideas for formulating more effective prevention and control strategies. Sucrose non-fermenting 1 (Snf1)...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2023.2235460 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849431710296440832 |
|---|---|
| author | Hui Wen Shuai Meng Shuwei Xie Huanbin Shi Jiehua Qiu Nan Jiang Yanjun Kou |
| author_facet | Hui Wen Shuai Meng Shuwei Xie Huanbin Shi Jiehua Qiu Nan Jiang Yanjun Kou |
| author_sort | Hui Wen |
| collection | DOAJ |
| description | Rice false smut caused by Ustilaginoidea virens is becoming one of the most devastating diseases in rice production areas in the world. Revealing U. virens potential pathogenic mechanisms provides ideas for formulating more effective prevention and control strategies. Sucrose non-fermenting 1 (Snf1) protein kinase plays a critical role in activating transcription and suppressing gene expression, as well as in cellular response to various stresses, such as nutrient limitation. In our study, we identified the Snf1 homolog UvSnf1 and analyzed its biological functions in U. virens. The expression level of UvSnf1 was dramatically up-regulated during invasion, indicating that UvSnf1 may participate in infection. Phenotypic analyses of UvSnf1 deletion mutants revealed that UvSnf1 is necessary for hyphae growth, spore production, and virulence in U. virens. Moreover, UvSnf1 promotes U. virens to use unfavorable carbon sources when the sucrose is insufficient. In addition, deletion of UvSnf1 down-regulates the expression of the cell wall-degrading enzymes (CWDEs) genes under sucrose limitation conditions in U. virens. Further analyses showed that CWDEs (UvCut1 and UvXyp1) are not only involved in growth, spore production, and virulence but are also required for the utilization of carbon sources. In conclusion, this study demonstrates that UvSnf1 plays vital roles in virulence and carbon source utilization in U. virens, and one of the possible mechanisms is playing a role in regulating the expression of CWDE genes. |
| format | Article |
| id | doaj-art-9aa5637a8b0e4d458c616a3a97a159a4 |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-9aa5637a8b0e4d458c616a3a97a159a42025-08-20T03:27:33ZengTaylor & Francis GroupVirulence2150-55942150-56082023-12-0114110.1080/21505594.2023.2235460Sucrose non-fermenting protein kinase gene UvSnf1 is required for virulence in Ustilaginoidea virensHui Wen0Shuai Meng1Shuwei Xie2Huanbin Shi3Jiehua Qiu4Nan Jiang5Yanjun Kou6State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, ChinaRice false smut caused by Ustilaginoidea virens is becoming one of the most devastating diseases in rice production areas in the world. Revealing U. virens potential pathogenic mechanisms provides ideas for formulating more effective prevention and control strategies. Sucrose non-fermenting 1 (Snf1) protein kinase plays a critical role in activating transcription and suppressing gene expression, as well as in cellular response to various stresses, such as nutrient limitation. In our study, we identified the Snf1 homolog UvSnf1 and analyzed its biological functions in U. virens. The expression level of UvSnf1 was dramatically up-regulated during invasion, indicating that UvSnf1 may participate in infection. Phenotypic analyses of UvSnf1 deletion mutants revealed that UvSnf1 is necessary for hyphae growth, spore production, and virulence in U. virens. Moreover, UvSnf1 promotes U. virens to use unfavorable carbon sources when the sucrose is insufficient. In addition, deletion of UvSnf1 down-regulates the expression of the cell wall-degrading enzymes (CWDEs) genes under sucrose limitation conditions in U. virens. Further analyses showed that CWDEs (UvCut1 and UvXyp1) are not only involved in growth, spore production, and virulence but are also required for the utilization of carbon sources. In conclusion, this study demonstrates that UvSnf1 plays vital roles in virulence and carbon source utilization in U. virens, and one of the possible mechanisms is playing a role in regulating the expression of CWDE genes.https://www.tandfonline.com/doi/10.1080/21505594.2023.2235460Plant diseaserice false smutpathogenicityvirulenceSnf1cell wall-degrading enzyme |
| spellingShingle | Hui Wen Shuai Meng Shuwei Xie Huanbin Shi Jiehua Qiu Nan Jiang Yanjun Kou Sucrose non-fermenting protein kinase gene UvSnf1 is required for virulence in Ustilaginoidea virens Virulence Plant disease rice false smut pathogenicity virulence Snf1 cell wall-degrading enzyme |
| title | Sucrose non-fermenting protein kinase gene UvSnf1 is required for virulence in Ustilaginoidea virens |
| title_full | Sucrose non-fermenting protein kinase gene UvSnf1 is required for virulence in Ustilaginoidea virens |
| title_fullStr | Sucrose non-fermenting protein kinase gene UvSnf1 is required for virulence in Ustilaginoidea virens |
| title_full_unstemmed | Sucrose non-fermenting protein kinase gene UvSnf1 is required for virulence in Ustilaginoidea virens |
| title_short | Sucrose non-fermenting protein kinase gene UvSnf1 is required for virulence in Ustilaginoidea virens |
| title_sort | sucrose non fermenting protein kinase gene uvsnf1 is required for virulence in ustilaginoidea virens |
| topic | Plant disease rice false smut pathogenicity virulence Snf1 cell wall-degrading enzyme |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2023.2235460 |
| work_keys_str_mv | AT huiwen sucrosenonfermentingproteinkinasegeneuvsnf1isrequiredforvirulenceinustilaginoideavirens AT shuaimeng sucrosenonfermentingproteinkinasegeneuvsnf1isrequiredforvirulenceinustilaginoideavirens AT shuweixie sucrosenonfermentingproteinkinasegeneuvsnf1isrequiredforvirulenceinustilaginoideavirens AT huanbinshi sucrosenonfermentingproteinkinasegeneuvsnf1isrequiredforvirulenceinustilaginoideavirens AT jiehuaqiu sucrosenonfermentingproteinkinasegeneuvsnf1isrequiredforvirulenceinustilaginoideavirens AT nanjiang sucrosenonfermentingproteinkinasegeneuvsnf1isrequiredforvirulenceinustilaginoideavirens AT yanjunkou sucrosenonfermentingproteinkinasegeneuvsnf1isrequiredforvirulenceinustilaginoideavirens |