Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
The membrane dipole potential that arises from the interfacial water and constitutive dipolar groups of lipid molecules modulates the interaction of amphiphiles and proteins with membranes. Consequently, its determination for lipid mixtures resembling the existing diversity in biological membranes i...
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MDPI AG
2024-12-01
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| author | Renato M. S. Cardoso Fabiana Lairion Edgardo Anibal Disalvo Luís M. S. Loura Maria João Moreno |
| author_facet | Renato M. S. Cardoso Fabiana Lairion Edgardo Anibal Disalvo Luís M. S. Loura Maria João Moreno |
| author_sort | Renato M. S. Cardoso |
| collection | DOAJ |
| description | The membrane dipole potential that arises from the interfacial water and constitutive dipolar groups of lipid molecules modulates the interaction of amphiphiles and proteins with membranes. Consequently, its determination for lipid mixtures resembling the existing diversity in biological membranes is very relevant. In this work, the dipole potentials of monolayers, formed at the air–water interface, from pure or mixed lipids (1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphatidyserine (POPS), sphingomyelin (SpM) and cholesterol) were measured and correlated with the mean area per lipid. The results showed that, as previously observed, cholesterol increases the dipole potential in correspondence with the decrease in the average area per lipid. At the small mole fractions encountered in biomembranes, the presence of the negatively charged lipid POPS increases the dipole potentials of monolayers despite inducing an increase in the average area per lipid. Additionally, the inclusion of POPE in POPC:cholesterol monolayers disrupts the area condensation induced by cholesterol while increasing the membrane dipole moment, leading to a small reduction in the dipole potential. This trend is reinforced for the quaternary POPC:cholesterol:POPE:POPS 4:3:2:1 system, which mimics the inner leaflets of eukaryotic plasma membranes. In agreement with previous works, the replacement of phosphocholine lipids with sphingomyelin leads to a decrease in the dipole potential. Together, this results in a lower dipole potential for the SpM-enriched outer leaflet, generating a non-zero transbilayer dipole potential in the asymmetric plasma membranes of eukaryotic cells. |
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| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-9aa198b9609e4b07995d2f9fc8b7e55e2025-08-20T02:01:28ZengMDPI AGMolecules1420-30492024-12-012924584310.3390/molecules29245843Dipole Potential of Monolayers with Biologically Relevant Lipid CompositionsRenato M. S. Cardoso0Fabiana Lairion1Edgardo Anibal Disalvo2Luís M. S. Loura3Maria João Moreno4Coimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, PortugalInstitute of Biochemistry and Molecular Medicine Prof. Alberto Boveris (IBIMOL), University of Buenos Aires and National Council for Scientific and Technical Research (CONICET), Buenos Aires 1113, ArgentinaApplied Biophysics and Food Research Center (CIBAAL), National University of Santiago del Estero and National Council for Scientific and Technical Research (CONICET), Santiago del Estero 4206, ArgentinaCoimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, PortugalCoimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, PortugalThe membrane dipole potential that arises from the interfacial water and constitutive dipolar groups of lipid molecules modulates the interaction of amphiphiles and proteins with membranes. Consequently, its determination for lipid mixtures resembling the existing diversity in biological membranes is very relevant. In this work, the dipole potentials of monolayers, formed at the air–water interface, from pure or mixed lipids (1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphatidyserine (POPS), sphingomyelin (SpM) and cholesterol) were measured and correlated with the mean area per lipid. The results showed that, as previously observed, cholesterol increases the dipole potential in correspondence with the decrease in the average area per lipid. At the small mole fractions encountered in biomembranes, the presence of the negatively charged lipid POPS increases the dipole potentials of monolayers despite inducing an increase in the average area per lipid. Additionally, the inclusion of POPE in POPC:cholesterol monolayers disrupts the area condensation induced by cholesterol while increasing the membrane dipole moment, leading to a small reduction in the dipole potential. This trend is reinforced for the quaternary POPC:cholesterol:POPE:POPS 4:3:2:1 system, which mimics the inner leaflets of eukaryotic plasma membranes. In agreement with previous works, the replacement of phosphocholine lipids with sphingomyelin leads to a decrease in the dipole potential. Together, this results in a lower dipole potential for the SpM-enriched outer leaflet, generating a non-zero transbilayer dipole potential in the asymmetric plasma membranes of eukaryotic cells.https://www.mdpi.com/1420-3049/29/24/5843transmembrane potentialcholesterolPOPCPOPEPOPSsurface pressure |
| spellingShingle | Renato M. S. Cardoso Fabiana Lairion Edgardo Anibal Disalvo Luís M. S. Loura Maria João Moreno Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions Molecules transmembrane potential cholesterol POPC POPE POPS surface pressure |
| title | Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions |
| title_full | Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions |
| title_fullStr | Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions |
| title_full_unstemmed | Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions |
| title_short | Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions |
| title_sort | dipole potential of monolayers with biologically relevant lipid compositions |
| topic | transmembrane potential cholesterol POPC POPE POPS surface pressure |
| url | https://www.mdpi.com/1420-3049/29/24/5843 |
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