Comprehensive Identification of Auxin Response Factor Gene Family and Their Expression Profiles Under Lanthanum Stress in <i>Amorphophallus konjac</i>
<i>Amorphophallus konjac</i> is an economically important horticultural crop, valued for its corms as both a traditional medicine and a food source. The auxin transcription factor (ARF) family plays pivotal roles in auxin signaling and the processes of the morphogenesis of tissues and or...
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2025-06-01
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| author | Xiaoxian Li Herui Huang Jinjun Shen Jingyi Long Hui Wang Zhenming Yu |
| author_facet | Xiaoxian Li Herui Huang Jinjun Shen Jingyi Long Hui Wang Zhenming Yu |
| author_sort | Xiaoxian Li |
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| description | <i>Amorphophallus konjac</i> is an economically important horticultural crop, valued for its corms as both a traditional medicine and a food source. The auxin transcription factor (ARF) family plays pivotal roles in auxin signaling and the processes of the morphogenesis of tissues and organs. However, the specific role of the <i>ARF</i> gene family in the regulation of corms remains unknown. In this study, we identified 23 <i>AkARF</i> genes unevenly distributed across 11 chromosomes in <i>A. konjac</i>. Phylogenetic analysis classified these proteins into four distinct clades, with members of the same group sharing conserved gene structures. Expression profiling demonstrated <i>AkARF</i> genes were expressed in a tissue-specific and spatio-temporal manner. Furthermore, lanthanum treatment significantly increased corm biomass and endogenous auxin levels (peak at 20 mg·L<sup>−1</sup>; <i>p</i> < 0.05). Transcriptome and qRT-PCR analyses revealed coordinated expression of <i>AkARF6/13/14/16/20</i> with corm biomass accumulation. Pearson’s correlation analysis further confirmed positive correlations of <i>AkARF6/13</i> with auxin content (<i>p</i> < 0.05). These results suggested the potential regulatory roles of <i>AkARF6/13</i> in auxin-mediated corm development. This study provides the potential functional role of ARF-mediated growth and development of corms in <i>A. konjac</i>. |
| format | Article |
| id | doaj-art-9a99e028aee64a73aed190685d6ce8ec |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-9a99e028aee64a73aed190685d6ce8ec2025-08-20T03:26:52ZengMDPI AGHorticulturae2311-75242025-06-0111668710.3390/horticulturae11060687Comprehensive Identification of Auxin Response Factor Gene Family and Their Expression Profiles Under Lanthanum Stress in <i>Amorphophallus konjac</i>Xiaoxian Li0Herui Huang1Jinjun Shen2Jingyi Long3Hui Wang4Zhenming Yu5School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China<i>Amorphophallus konjac</i> is an economically important horticultural crop, valued for its corms as both a traditional medicine and a food source. The auxin transcription factor (ARF) family plays pivotal roles in auxin signaling and the processes of the morphogenesis of tissues and organs. However, the specific role of the <i>ARF</i> gene family in the regulation of corms remains unknown. In this study, we identified 23 <i>AkARF</i> genes unevenly distributed across 11 chromosomes in <i>A. konjac</i>. Phylogenetic analysis classified these proteins into four distinct clades, with members of the same group sharing conserved gene structures. Expression profiling demonstrated <i>AkARF</i> genes were expressed in a tissue-specific and spatio-temporal manner. Furthermore, lanthanum treatment significantly increased corm biomass and endogenous auxin levels (peak at 20 mg·L<sup>−1</sup>; <i>p</i> < 0.05). Transcriptome and qRT-PCR analyses revealed coordinated expression of <i>AkARF6/13/14/16/20</i> with corm biomass accumulation. Pearson’s correlation analysis further confirmed positive correlations of <i>AkARF6/13</i> with auxin content (<i>p</i> < 0.05). These results suggested the potential regulatory roles of <i>AkARF6/13</i> in auxin-mediated corm development. This study provides the potential functional role of ARF-mediated growth and development of corms in <i>A. konjac</i>.https://www.mdpi.com/2311-7524/11/6/687auxin response factorsgene family<i>Amorphophallus konjac</i>expression pattern analysis |
| spellingShingle | Xiaoxian Li Herui Huang Jinjun Shen Jingyi Long Hui Wang Zhenming Yu Comprehensive Identification of Auxin Response Factor Gene Family and Their Expression Profiles Under Lanthanum Stress in <i>Amorphophallus konjac</i> Horticulturae auxin response factors gene family <i>Amorphophallus konjac</i> expression pattern analysis |
| title | Comprehensive Identification of Auxin Response Factor Gene Family and Their Expression Profiles Under Lanthanum Stress in <i>Amorphophallus konjac</i> |
| title_full | Comprehensive Identification of Auxin Response Factor Gene Family and Their Expression Profiles Under Lanthanum Stress in <i>Amorphophallus konjac</i> |
| title_fullStr | Comprehensive Identification of Auxin Response Factor Gene Family and Their Expression Profiles Under Lanthanum Stress in <i>Amorphophallus konjac</i> |
| title_full_unstemmed | Comprehensive Identification of Auxin Response Factor Gene Family and Their Expression Profiles Under Lanthanum Stress in <i>Amorphophallus konjac</i> |
| title_short | Comprehensive Identification of Auxin Response Factor Gene Family and Their Expression Profiles Under Lanthanum Stress in <i>Amorphophallus konjac</i> |
| title_sort | comprehensive identification of auxin response factor gene family and their expression profiles under lanthanum stress in i amorphophallus konjac i |
| topic | auxin response factors gene family <i>Amorphophallus konjac</i> expression pattern analysis |
| url | https://www.mdpi.com/2311-7524/11/6/687 |
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