Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiae
Pseudomonas syringae pv. actinidiae (Psa), the etiological agent of the bacterial canker in Actinidia plants, remains the main threat to kiwifruit orchards worldwide. Though environment-friendly disease control methods based on biological control agents (BCAs) represent a promising alternative to xe...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425000167 |
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| author | Cristiana Correia Antonio Cellini Irene Donati Panagiotis Voulgaris Adebayo Ebenezer Obafemi Elia Soriato Elodie Vandelle Conceição Santos Francesco Spinelli |
| author_facet | Cristiana Correia Antonio Cellini Irene Donati Panagiotis Voulgaris Adebayo Ebenezer Obafemi Elia Soriato Elodie Vandelle Conceição Santos Francesco Spinelli |
| author_sort | Cristiana Correia |
| collection | DOAJ |
| description | Pseudomonas syringae pv. actinidiae (Psa), the etiological agent of the bacterial canker in Actinidia plants, remains the main threat to kiwifruit orchards worldwide. Though environment-friendly disease control methods based on biological control agents (BCAs) represent a promising alternative to xenobiotic pesticides, their efficacy in field conditions has often resulted erratic. The selection of beneficial microorganisms directly from the phyllosphere of the host plant is a promising approach to overcome this limitation since it ensures the adaptation of the isolates to the environment in which they are going to be applied. This work reports the screening of the kiwifruit epiphytic bacterial community from three Psa infected orchards in Portugal to identify potential bacterial BCAs capable of inhibiting Psa growth or interfering with its virulence. Strains of Pseudomonas putida and Pseudomonas azotoformans efficiently antagonized Psa on flowers and leaves and colonized all susceptible organs with high surviving rates in glasshouse conditions. In vitro metabolic analysis together with genome sequencing and annotation revealed siderophore production, in particular pyoverdine, which may limit iron availability to the pathogen. Moreover, several biosynthetic gene clusters of secondary metabolites, were predicted in the genome of both strains, including non-ribosomal peptides, and the bacteriocin pyocin was predicted in the genome of BG1. Overall, these results open new perspectives to develop commercial products for Psa management based on kiwifruit-native bacteria, well-adapted to common orchard management practices, with a high efficiency of host plant colonization, at Psa-conducive temperatures, and point out possible mechanisms of action for these two BCA candidates, supporting further steps to assess their effectiveness in orchard conditions. |
| format | Article |
| id | doaj-art-9a8ec3e1c4f441afa5377ce275decb51 |
| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-9a8ec3e1c4f441afa5377ce275decb512025-02-05T04:31:13ZengElsevierBiological Control1049-96442025-02-01201105706Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiaeCristiana Correia0Antonio Cellini1Irene Donati2Panagiotis Voulgaris3Adebayo Ebenezer Obafemi4Elia Soriato5Elodie Vandelle6Conceição Santos7Francesco Spinelli8Department of Agricultural Sciences, Alma Mater Studiorum University of Bologna, Viale Fanin 46, 40127 Bologna, Italy; Department of Biology, LAQV-REQUIMTE, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalDepartment of Agricultural Sciences, Alma Mater Studiorum University of Bologna, Viale Fanin 46, 40127 Bologna, ItalyDepartment of Agricultural Sciences, Alma Mater Studiorum University of Bologna, Viale Fanin 46, 40127 Bologna, ItalyDepartment of Agricultural Sciences, Alma Mater Studiorum University of Bologna, Viale Fanin 46, 40127 Bologna, ItalyDepartment of Agricultural Sciences, Alma Mater Studiorum University of Bologna, Viale Fanin 46, 40127 Bologna, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie, 15, Verona 37134, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie, 15, Verona 37134, ItalyDepartment of Biology, LAQV-REQUIMTE, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalDepartment of Agricultural Sciences, Alma Mater Studiorum University of Bologna, Viale Fanin 46, 40127 Bologna, Italy; Corresponding author.Pseudomonas syringae pv. actinidiae (Psa), the etiological agent of the bacterial canker in Actinidia plants, remains the main threat to kiwifruit orchards worldwide. Though environment-friendly disease control methods based on biological control agents (BCAs) represent a promising alternative to xenobiotic pesticides, their efficacy in field conditions has often resulted erratic. The selection of beneficial microorganisms directly from the phyllosphere of the host plant is a promising approach to overcome this limitation since it ensures the adaptation of the isolates to the environment in which they are going to be applied. This work reports the screening of the kiwifruit epiphytic bacterial community from three Psa infected orchards in Portugal to identify potential bacterial BCAs capable of inhibiting Psa growth or interfering with its virulence. Strains of Pseudomonas putida and Pseudomonas azotoformans efficiently antagonized Psa on flowers and leaves and colonized all susceptible organs with high surviving rates in glasshouse conditions. In vitro metabolic analysis together with genome sequencing and annotation revealed siderophore production, in particular pyoverdine, which may limit iron availability to the pathogen. Moreover, several biosynthetic gene clusters of secondary metabolites, were predicted in the genome of both strains, including non-ribosomal peptides, and the bacteriocin pyocin was predicted in the genome of BG1. Overall, these results open new perspectives to develop commercial products for Psa management based on kiwifruit-native bacteria, well-adapted to common orchard management practices, with a high efficiency of host plant colonization, at Psa-conducive temperatures, and point out possible mechanisms of action for these two BCA candidates, supporting further steps to assess their effectiveness in orchard conditions.http://www.sciencedirect.com/science/article/pii/S1049964425000167Bacterial cankerBiocontrolNative bacteriaPhyllosphereProtection |
| spellingShingle | Cristiana Correia Antonio Cellini Irene Donati Panagiotis Voulgaris Adebayo Ebenezer Obafemi Elia Soriato Elodie Vandelle Conceição Santos Francesco Spinelli Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiae Biological Control Bacterial canker Biocontrol Native bacteria Phyllosphere Protection |
| title | Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiae |
| title_full | Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiae |
| title_fullStr | Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiae |
| title_full_unstemmed | Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiae |
| title_short | Pseudomonas azotoformans and Pseudomonas putida: Novel kiwifruit-native biological control agents against Pseudomonas syringae pv. actinidiae |
| title_sort | pseudomonas azotoformans and pseudomonas putida novel kiwifruit native biological control agents against pseudomonas syringae pv actinidiae |
| topic | Bacterial canker Biocontrol Native bacteria Phyllosphere Protection |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425000167 |
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