Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic Reaction

A simple, accurate and sensitive spectrophotometric way is used to determine Bisacodyl in pure and pharmaceutical preparations. The proposed method depends on using 2,4-Dinitrophenylhydrazine as chromogenic reagent . The method was based on the oxidative coupling reaction of Bisacodyl with 2,4-Dinit...

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Main Author: Baghdad Science Journal
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2017-03-01
Series:مجلة بغداد للعلوم
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Online Access:http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2351
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author Baghdad Science Journal
author_facet Baghdad Science Journal
author_sort Baghdad Science Journal
collection DOAJ
description A simple, accurate and sensitive spectrophotometric way is used to determine Bisacodyl in pure and pharmaceutical preparations. The proposed method depends on using 2,4-Dinitrophenylhydrazine as chromogenic reagent . The method was based on the oxidative coupling reaction of Bisacodyl with 2,4-Dinitrophenylhydrazine with Sodium periodate in the presence of sodium hydroxide as alkaline media to form red water soluble dye product , that has a maximum absorption at ?max 522nm . Beer ,s law is obeyed in the concentration of (2.00–20.00) ?g.ml -1 .The molar absorptivity is (6505) L.mol-1.cm-1,a sandall sensitivity of(0.0555) ?g.cm-2), correlation coefficient of (0.9970) , Limitof detection (LOD) (0.0312 ?g.ml-1), limit of Quantitation (LOQ) (0. 3125 ?g.ml-1) and the relative standard deviation of RSD% (1.6). The method gave a successful determination for Bisacodyl in pharmaceutical preparations and the value of recovery % was better than (100.16%)
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institution Kabale University
issn 2078-8665
2411-7986
language English
publishDate 2017-03-01
publisher University of Baghdad, College of Science for Women
record_format Article
series مجلة بغداد للعلوم
spelling doaj-art-9a8a407bc8c64f31be69859231a5edd32025-08-20T03:33:46ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862017-03-0114110.21123/bsj.14.1.181-188Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic ReactionBaghdad Science JournalA simple, accurate and sensitive spectrophotometric way is used to determine Bisacodyl in pure and pharmaceutical preparations. The proposed method depends on using 2,4-Dinitrophenylhydrazine as chromogenic reagent . The method was based on the oxidative coupling reaction of Bisacodyl with 2,4-Dinitrophenylhydrazine with Sodium periodate in the presence of sodium hydroxide as alkaline media to form red water soluble dye product , that has a maximum absorption at ?max 522nm . Beer ,s law is obeyed in the concentration of (2.00–20.00) ?g.ml -1 .The molar absorptivity is (6505) L.mol-1.cm-1,a sandall sensitivity of(0.0555) ?g.cm-2), correlation coefficient of (0.9970) , Limitof detection (LOD) (0.0312 ?g.ml-1), limit of Quantitation (LOQ) (0. 3125 ?g.ml-1) and the relative standard deviation of RSD% (1.6). The method gave a successful determination for Bisacodyl in pharmaceutical preparations and the value of recovery % was better than (100.16%)http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2351Bisacodyl drug, Spectrophotometric determination, Pharmaceutical preparation
spellingShingle Baghdad Science Journal
Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic Reaction
مجلة بغداد للعلوم
Bisacodyl drug, Spectrophotometric determination, Pharmaceutical preparation
title Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic Reaction
title_full Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic Reaction
title_fullStr Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic Reaction
title_full_unstemmed Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic Reaction
title_short Spectrophotometric Determination of Bisacodyl in Pure and Pharmaceutical Preparation via Oxidative Coupling Organic Reaction
title_sort spectrophotometric determination of bisacodyl in pure and pharmaceutical preparation via oxidative coupling organic reaction
topic Bisacodyl drug, Spectrophotometric determination, Pharmaceutical preparation
url http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2351
work_keys_str_mv AT baghdadsciencejournal spectrophotometricdeterminationofbisacodylinpureandpharmaceuticalpreparationviaoxidativecouplingorganicreaction