Vascular bundle cell-specific expression of a phosphate transporter improves phosphate use efficiency of transgenic Arabidopsis without detrimental effects
Abstract Constitutive overexpression of phosphate (Pi) transporter family 1 often results in the accumulation of toxic levels of Pi, which causes growth retardation in plants. In contrast, we had previously reported that root epidermis-specific overexpression of the phosphate transporter TaPT2 in Ar...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-78500-5 |
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| author | Yuichi Tada Aoi Shimizu |
| author_facet | Yuichi Tada Aoi Shimizu |
| author_sort | Yuichi Tada |
| collection | DOAJ |
| description | Abstract Constitutive overexpression of phosphate (Pi) transporter family 1 often results in the accumulation of toxic levels of Pi, which causes growth retardation in plants. In contrast, we had previously reported that root epidermis-specific overexpression of the phosphate transporter TaPT2 in Arabidopsis leads to improved growth and Pi use efficiency. In the present study, we used promoters AtHKT1;1 and SKOR, which are predominantly expressed in the vascular bundle tissues, to overexpress TaPT2. Transgenic lines exhibited increased shoot growth compared to wild type plants under normal- and low-Pi conditions, along with elevated root Pi and total P content, and higher xylem sap Pi concentration, specifically under low-Pi conditions. This was attributed to moderate Pi accumulation in the xylem parenchyma cells, enhancing the Pi uploading capacity to the xylem. SKOR-TaPT2, however, did not complement pho1 mutant, which was defective in uploading Pi to the xylem. The transcriptional levels of VPT1 and VPT3, which are responsible for transporting excess Pi into a vacuole, were upregulated in SKOR promoter lines under normal-Pi conditions. Our results suggested that root vascular bundle-specific expression of TaPT2 is another promising strategy for increasing biomass production, Pi uptake, and Pi use efficiency while preventing growth retardation in transgenic plants. |
| format | Article |
| id | doaj-art-9a7e5ee57e074ae1af1f095fb31524f0 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-9a7e5ee57e074ae1af1f095fb31524f02025-08-20T02:50:04ZengNature PortfolioScientific Reports2045-23222024-11-0114111010.1038/s41598-024-78500-5Vascular bundle cell-specific expression of a phosphate transporter improves phosphate use efficiency of transgenic Arabidopsis without detrimental effectsYuichi Tada0Aoi Shimizu1School of Bioscience and Biotechnology, Tokyo University of TechnologyGraduate School of Bionics, Tokyo University of TechnologyAbstract Constitutive overexpression of phosphate (Pi) transporter family 1 often results in the accumulation of toxic levels of Pi, which causes growth retardation in plants. In contrast, we had previously reported that root epidermis-specific overexpression of the phosphate transporter TaPT2 in Arabidopsis leads to improved growth and Pi use efficiency. In the present study, we used promoters AtHKT1;1 and SKOR, which are predominantly expressed in the vascular bundle tissues, to overexpress TaPT2. Transgenic lines exhibited increased shoot growth compared to wild type plants under normal- and low-Pi conditions, along with elevated root Pi and total P content, and higher xylem sap Pi concentration, specifically under low-Pi conditions. This was attributed to moderate Pi accumulation in the xylem parenchyma cells, enhancing the Pi uploading capacity to the xylem. SKOR-TaPT2, however, did not complement pho1 mutant, which was defective in uploading Pi to the xylem. The transcriptional levels of VPT1 and VPT3, which are responsible for transporting excess Pi into a vacuole, were upregulated in SKOR promoter lines under normal-Pi conditions. Our results suggested that root vascular bundle-specific expression of TaPT2 is another promising strategy for increasing biomass production, Pi uptake, and Pi use efficiency while preventing growth retardation in transgenic plants.https://doi.org/10.1038/s41598-024-78500-5Growth promotionPhosphate transporterPi acquisitionPi use efficiencyVascular bundle cell-specific expression |
| spellingShingle | Yuichi Tada Aoi Shimizu Vascular bundle cell-specific expression of a phosphate transporter improves phosphate use efficiency of transgenic Arabidopsis without detrimental effects Scientific Reports Growth promotion Phosphate transporter Pi acquisition Pi use efficiency Vascular bundle cell-specific expression |
| title | Vascular bundle cell-specific expression of a phosphate transporter improves phosphate use efficiency of transgenic Arabidopsis without detrimental effects |
| title_full | Vascular bundle cell-specific expression of a phosphate transporter improves phosphate use efficiency of transgenic Arabidopsis without detrimental effects |
| title_fullStr | Vascular bundle cell-specific expression of a phosphate transporter improves phosphate use efficiency of transgenic Arabidopsis without detrimental effects |
| title_full_unstemmed | Vascular bundle cell-specific expression of a phosphate transporter improves phosphate use efficiency of transgenic Arabidopsis without detrimental effects |
| title_short | Vascular bundle cell-specific expression of a phosphate transporter improves phosphate use efficiency of transgenic Arabidopsis without detrimental effects |
| title_sort | vascular bundle cell specific expression of a phosphate transporter improves phosphate use efficiency of transgenic arabidopsis without detrimental effects |
| topic | Growth promotion Phosphate transporter Pi acquisition Pi use efficiency Vascular bundle cell-specific expression |
| url | https://doi.org/10.1038/s41598-024-78500-5 |
| work_keys_str_mv | AT yuichitada vascularbundlecellspecificexpressionofaphosphatetransporterimprovesphosphateuseefficiencyoftransgenicarabidopsiswithoutdetrimentaleffects AT aoishimizu vascularbundlecellspecificexpressionofaphosphatetransporterimprovesphosphateuseefficiencyoftransgenicarabidopsiswithoutdetrimentaleffects |