Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test
Background. Early detection of Gram-positive bacteremia and timely appropriate antimicrobial therapy are required for decreasing patient mortality. The purpose of our study was to evaluate the performance of the Verigene Gram-positive blood culture assay (BC-GP) in two special healthcare settings an...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/4896791 |
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| author | Ken Kikuchi Mari Matsuda Shigekazu Iguchi Tomonori Mizutani Keiichi Hiramatsu Michiru Tega-Ishii Kaori Sansaka Kenta Negishi Kimie Shimada Jun Umemura Shigeyuki Notake Hideji Yanagisawa Hiroshi Takahashi Reiko Yabusaki Hideki Araoka Akiko Yoneyama |
| author_facet | Ken Kikuchi Mari Matsuda Shigekazu Iguchi Tomonori Mizutani Keiichi Hiramatsu Michiru Tega-Ishii Kaori Sansaka Kenta Negishi Kimie Shimada Jun Umemura Shigeyuki Notake Hideji Yanagisawa Hiroshi Takahashi Reiko Yabusaki Hideki Araoka Akiko Yoneyama |
| author_sort | Ken Kikuchi |
| collection | DOAJ |
| description | Background. Early detection of Gram-positive bacteremia and timely appropriate antimicrobial therapy are required for decreasing patient mortality. The purpose of our study was to evaluate the performance of the Verigene Gram-positive blood culture assay (BC-GP) in two special healthcare settings and determine the potential impact of rapid blood culture testing for Gram-positive bacteremia within the Japanese healthcare delivery system. Furthermore, the study included simulated blood cultures, which included a library of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) isolates reflecting different geographical regions in Japan. Methods. A total 347 BC-GP assays were performed on clinical and simulated blood cultures. BC-GP results were compared to results obtained by reference methods for genus/species identification and detection of resistance genes using molecular and MALDI-TOF MS methodologies. Results. For identification and detection of resistance genes at two clinical sites and simulated blood cultures, overall concordance of BC-GP with reference methods was 327/347 (94%). The time for identification and antimicrobial resistance detection by BC-GP was significantly shorter compared to routine testing especially at the cardiology hospital, which does not offer clinical microbiology services on weekends and holidays. Conclusion. BC-GP generated accurate identification and detection of resistance markers compared with routine laboratory methods for Gram-positive organisms in specialized clinical settings providing more rapid results than current routine testing. |
| format | Article |
| id | doaj-art-9a7bb903dcb6494b8f82b0bb74000981 |
| institution | OA Journals |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-9a7bb903dcb6494b8f82b0bb740009812025-08-20T02:20:15ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932017-01-01201710.1155/2017/48967914896791Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture TestKen Kikuchi0Mari Matsuda1Shigekazu Iguchi2Tomonori Mizutani3Keiichi Hiramatsu4Michiru Tega-Ishii5Kaori Sansaka6Kenta Negishi7Kimie Shimada8Jun Umemura9Shigeyuki Notake10Hideji Yanagisawa11Hiroshi Takahashi12Reiko Yabusaki13Hideki Araoka14Akiko Yoneyama15Department of Infectious Diseases, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanDepartment of Infection Control Science, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, JapanDepartment of Infectious Diseases, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, JapanDepartment of Infection Control Science, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, JapanDepartment of Infection Control Science, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, JapanSakakibara Heart Institute, 1-16-1 Asahimachi, Fuchu, Tokyo 183-0003, JapanSakakibara Heart Institute, 1-16-1 Asahimachi, Fuchu, Tokyo 183-0003, JapanSakakibara Heart Institute, 1-16-1 Asahimachi, Fuchu, Tokyo 183-0003, JapanSakakibara Heart Institute, 1-16-1 Asahimachi, Fuchu, Tokyo 183-0003, JapanSakakibara Heart Institute, 1-16-1 Asahimachi, Fuchu, Tokyo 183-0003, JapanMiroku Medical Laboratory Inc., 659-2 Innai, Saku, Nagano 384-2201, JapanMiroku Medical Laboratory Inc., 659-2 Innai, Saku, Nagano 384-2201, JapanEast-West Diagnostics/Theranostics, San Francisco, CA 94105, USADepartment of Infectious Diseases, Toranomon Hospital, 2-2-2 Toranomon, Minato-ku, Tokyo 105-8470, JapanDepartment of Infectious Diseases, Toranomon Hospital, 2-2-2 Toranomon, Minato-ku, Tokyo 105-8470, JapanDepartment of Infectious Diseases, Toranomon Hospital, 2-2-2 Toranomon, Minato-ku, Tokyo 105-8470, JapanBackground. Early detection of Gram-positive bacteremia and timely appropriate antimicrobial therapy are required for decreasing patient mortality. The purpose of our study was to evaluate the performance of the Verigene Gram-positive blood culture assay (BC-GP) in two special healthcare settings and determine the potential impact of rapid blood culture testing for Gram-positive bacteremia within the Japanese healthcare delivery system. Furthermore, the study included simulated blood cultures, which included a library of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) isolates reflecting different geographical regions in Japan. Methods. A total 347 BC-GP assays were performed on clinical and simulated blood cultures. BC-GP results were compared to results obtained by reference methods for genus/species identification and detection of resistance genes using molecular and MALDI-TOF MS methodologies. Results. For identification and detection of resistance genes at two clinical sites and simulated blood cultures, overall concordance of BC-GP with reference methods was 327/347 (94%). The time for identification and antimicrobial resistance detection by BC-GP was significantly shorter compared to routine testing especially at the cardiology hospital, which does not offer clinical microbiology services on weekends and holidays. Conclusion. BC-GP generated accurate identification and detection of resistance markers compared with routine laboratory methods for Gram-positive organisms in specialized clinical settings providing more rapid results than current routine testing.http://dx.doi.org/10.1155/2017/4896791 |
| spellingShingle | Ken Kikuchi Mari Matsuda Shigekazu Iguchi Tomonori Mizutani Keiichi Hiramatsu Michiru Tega-Ishii Kaori Sansaka Kenta Negishi Kimie Shimada Jun Umemura Shigeyuki Notake Hideji Yanagisawa Hiroshi Takahashi Reiko Yabusaki Hideki Araoka Akiko Yoneyama Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test |
| title_full | Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test |
| title_fullStr | Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test |
| title_full_unstemmed | Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test |
| title_short | Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test |
| title_sort | potential impact of rapid blood culture testing for gram positive bacteremia in japan with the verigene gram positive blood culture test |
| url | http://dx.doi.org/10.1155/2017/4896791 |
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