Lower Serum Free Amino Acids in Holstein Cows with Milk Fever in Field Case

This study was conducted to investigate the relationship between serum free amino acids (FAA) and the onset of milk fever in dairy cows around the time of calving. The serum concentration of FAA was compared between multiparous cows with milk fever (MF group; n=7) and healthy multiparous cows (Contr...

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Main Authors: Kudo Katsunori, Ohtsuka Hiromichi
Format: Article
Language:English
Published: Sciendo 2025-06-01
Series:Acta Veterinaria
Subjects:
Online Access:https://doi.org/10.2478/acve-2025-0020
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author Kudo Katsunori
Ohtsuka Hiromichi
author_facet Kudo Katsunori
Ohtsuka Hiromichi
author_sort Kudo Katsunori
collection DOAJ
description This study was conducted to investigate the relationship between serum free amino acids (FAA) and the onset of milk fever in dairy cows around the time of calving. The serum concentration of FAA was compared between multiparous cows with milk fever (MF group; n=7) and healthy multiparous cows (Control Group; N=16) kept on a Holstein dairy farm. Blood samples were obtained 3 to 4 weeks prepartum, on the day of calving, and 1 month post partum for analysis of serum amino acids and lipid parameters. The levels of all essential amino acids in the MF group were lower than those in the Control group. Significant differences between groups were observed in phenylalanine, valine, isoleucine, and leucine at 1 month post partum, and in tryptophan on the day of calving. Regarding the non essential amino acids, a significantly lower level of arginine was observed in the MF group compared to the Control group on the day of caving, as well as 1 month post partum. A significant positive correlation was detected between cholesterol and branched chain amino acids (BCAA) levels (r=0.531, P<0.01). These results showed that MF cows had lower levels in several FAA around the time of calving even before the onset of MF.
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spelling doaj-art-9a698ab74c2d4be498e138fc3fb4810c2025-08-20T02:37:13ZengSciendoActa Veterinaria1820-74482025-06-0175225927010.2478/acve-2025-0020Lower Serum Free Amino Acids in Holstein Cows with Milk Fever in Field CaseKudo Katsunori0Ohtsuka Hiromichi11Hokkaido Minami Agricultural Mutual Relief Association, Chitose, Hokkaido 066-00812Department of Veterinary Medicine, School of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicne, Inada-cho, Obihiro, Hokkaido 080-8555, JapanThis study was conducted to investigate the relationship between serum free amino acids (FAA) and the onset of milk fever in dairy cows around the time of calving. The serum concentration of FAA was compared between multiparous cows with milk fever (MF group; n=7) and healthy multiparous cows (Control Group; N=16) kept on a Holstein dairy farm. Blood samples were obtained 3 to 4 weeks prepartum, on the day of calving, and 1 month post partum for analysis of serum amino acids and lipid parameters. The levels of all essential amino acids in the MF group were lower than those in the Control group. Significant differences between groups were observed in phenylalanine, valine, isoleucine, and leucine at 1 month post partum, and in tryptophan on the day of calving. Regarding the non essential amino acids, a significantly lower level of arginine was observed in the MF group compared to the Control group on the day of caving, as well as 1 month post partum. A significant positive correlation was detected between cholesterol and branched chain amino acids (BCAA) levels (r=0.531, P<0.01). These results showed that MF cows had lower levels in several FAA around the time of calving even before the onset of MF.https://doi.org/10.2478/acve-2025-0020calvingdairy cowfree amino acidsmilk fever
spellingShingle Kudo Katsunori
Ohtsuka Hiromichi
Lower Serum Free Amino Acids in Holstein Cows with Milk Fever in Field Case
Acta Veterinaria
calving
dairy cow
free amino acids
milk fever
title Lower Serum Free Amino Acids in Holstein Cows with Milk Fever in Field Case
title_full Lower Serum Free Amino Acids in Holstein Cows with Milk Fever in Field Case
title_fullStr Lower Serum Free Amino Acids in Holstein Cows with Milk Fever in Field Case
title_full_unstemmed Lower Serum Free Amino Acids in Holstein Cows with Milk Fever in Field Case
title_short Lower Serum Free Amino Acids in Holstein Cows with Milk Fever in Field Case
title_sort lower serum free amino acids in holstein cows with milk fever in field case
topic calving
dairy cow
free amino acids
milk fever
url https://doi.org/10.2478/acve-2025-0020
work_keys_str_mv AT kudokatsunori lowerserumfreeaminoacidsinholsteincowswithmilkfeverinfieldcase
AT ohtsukahiromichi lowerserumfreeaminoacidsinholsteincowswithmilkfeverinfieldcase