Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice
Dimethyl sulfoxide (DMSO) is a commonly used solvent in life sciences due to its excellent ability to dissolve compounds with poor water-solubility. Depending on the applied dose, the variety of DMSO’s physiological and biological effects may compromise its suitability as a vehicle molecule. Even lo...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | IBRO Neuroscience Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266724212500034X |
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| author | Jeroen Spanoghe Arne Van Acker Evelien Carrette Kristl Vonck Paul Boon Robrecht Raedt |
| author_facet | Jeroen Spanoghe Arne Van Acker Evelien Carrette Kristl Vonck Paul Boon Robrecht Raedt |
| author_sort | Jeroen Spanoghe |
| collection | DOAJ |
| description | Dimethyl sulfoxide (DMSO) is a commonly used solvent in life sciences due to its excellent ability to dissolve compounds with poor water-solubility. Depending on the applied dose, the variety of DMSO’s physiological and biological effects may compromise its suitability as a vehicle molecule. Even low concentrations of DMSO are known to affect neuronal excitability in vitro. As in vivo effects have not been studied extensively, this exploratory study investigated the effects of intracerebroventricular (ICV) administration of different DMSO concentrations on hippocampal electrophysiology in mice. Acute recordings of hippocampal evoked potentials (EPs) and electroencephalography (EEG) were performed before and after ICV injection of a 5 µl DMSO solution, with concentrations ranging from 2.5 % to 100 % DMSO. Solutions containing up to 50 % DMSO had no acute effects on hippocampal electrophysiology. Administration of 75 % and 100 % DMSO was found to alter evoked responses, indicating increased excitability. Our results indicate that DMSO can be used as a vehicle in volumes of 5 µl containing concentrations of up to 50 % without affecting acute hippocampal electrophysiological studies in mice. Higher concentrations should be avoided as these affect neuronal excitability. |
| format | Article |
| id | doaj-art-9a5155d51919470da28af6f4c06f3d29 |
| institution | OA Journals |
| issn | 2667-2421 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | IBRO Neuroscience Reports |
| spelling | doaj-art-9a5155d51919470da28af6f4c06f3d292025-08-20T02:06:19ZengElsevierIBRO Neuroscience Reports2667-24212025-06-011837838310.1016/j.ibneur.2025.02.016Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in miceJeroen Spanoghe0Arne Van Acker1Evelien Carrette2Kristl Vonck3Paul Boon4Robrecht Raedt54Brain, Department of Head and Skin, Ghent University, Corneel Heymanslaan 10, Ghent 9000, Belgium4Brain, Department of Head and Skin, Ghent University, Corneel Heymanslaan 10, Ghent 9000, Belgium4Brain, Department of Head and Skin, Ghent University, Corneel Heymanslaan 10, Ghent 9000, Belgium4Brain, Department of Head and Skin, Ghent University, Corneel Heymanslaan 10, Ghent 9000, Belgium4Brain, Department of Head and Skin, Ghent University, Corneel Heymanslaan 10, Ghent 9000, BelgiumCorresponding author.; 4Brain, Department of Head and Skin, Ghent University, Corneel Heymanslaan 10, Ghent 9000, BelgiumDimethyl sulfoxide (DMSO) is a commonly used solvent in life sciences due to its excellent ability to dissolve compounds with poor water-solubility. Depending on the applied dose, the variety of DMSO’s physiological and biological effects may compromise its suitability as a vehicle molecule. Even low concentrations of DMSO are known to affect neuronal excitability in vitro. As in vivo effects have not been studied extensively, this exploratory study investigated the effects of intracerebroventricular (ICV) administration of different DMSO concentrations on hippocampal electrophysiology in mice. Acute recordings of hippocampal evoked potentials (EPs) and electroencephalography (EEG) were performed before and after ICV injection of a 5 µl DMSO solution, with concentrations ranging from 2.5 % to 100 % DMSO. Solutions containing up to 50 % DMSO had no acute effects on hippocampal electrophysiology. Administration of 75 % and 100 % DMSO was found to alter evoked responses, indicating increased excitability. Our results indicate that DMSO can be used as a vehicle in volumes of 5 µl containing concentrations of up to 50 % without affecting acute hippocampal electrophysiological studies in mice. Higher concentrations should be avoided as these affect neuronal excitability.http://www.sciencedirect.com/science/article/pii/S266724212500034XDimethyl sulfoxideElectrophysiologyHippocampusEvoked potentials |
| spellingShingle | Jeroen Spanoghe Arne Van Acker Evelien Carrette Kristl Vonck Paul Boon Robrecht Raedt Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice IBRO Neuroscience Reports Dimethyl sulfoxide Electrophysiology Hippocampus Evoked potentials |
| title | Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice |
| title_full | Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice |
| title_fullStr | Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice |
| title_full_unstemmed | Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice |
| title_short | Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice |
| title_sort | effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice |
| topic | Dimethyl sulfoxide Electrophysiology Hippocampus Evoked potentials |
| url | http://www.sciencedirect.com/science/article/pii/S266724212500034X |
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