AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease

Summary: The pathogenic factors of inflammatory bowel disease (IBD) are very complex, and further investigation of its pathogenesis is necessary. Some polar proteins have been reported to perform functions other than maintaining cell structure. In this study, we reported that the polar protein AF6 e...

Full description

Saved in:
Bibliographic Details
Main Authors: Liying Lu, Keying Xu, Huiheng Qu, Lele Song, Yanan Song, Yanjun Wu, Xia Sun, Jingwen Kong, Qian Wen, Jiazheng Jiao, Zhanju Liu, Jiazeng Xia, Lixing Zhan
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:iScience
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004225009198
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849708315851882496
author Liying Lu
Keying Xu
Huiheng Qu
Lele Song
Yanan Song
Yanjun Wu
Xia Sun
Jingwen Kong
Qian Wen
Jiazheng Jiao
Zhanju Liu
Jiazeng Xia
Lixing Zhan
author_facet Liying Lu
Keying Xu
Huiheng Qu
Lele Song
Yanan Song
Yanjun Wu
Xia Sun
Jingwen Kong
Qian Wen
Jiazheng Jiao
Zhanju Liu
Jiazeng Xia
Lixing Zhan
author_sort Liying Lu
collection DOAJ
description Summary: The pathogenic factors of inflammatory bowel disease (IBD) are very complex, and further investigation of its pathogenesis is necessary. Some polar proteins have been reported to perform functions other than maintaining cell structure. In this study, we reported that the polar protein AF6 expressed by intestinal epithelial cells (IEC) can regulate the expression of MHCII in epithelial cells, thus affecting the cross dialogue between IECs and immune cells, and promoting the secretion of IgA by B cells to play a protective function. IgA supplementation can also improve the severe colitis phenotype caused by AF6 loss. Our study provides an understanding of the regulatory mechanisms of the intestinal epithelial expression of MHC II and its important role in colitis, which will aid in treatment and intervention.
format Article
id doaj-art-9a4f97e0a7604812bdff207c2ff7108a
institution DOAJ
issn 2589-0042
language English
publishDate 2025-07-01
publisher Elsevier
record_format Article
series iScience
spelling doaj-art-9a4f97e0a7604812bdff207c2ff7108a2025-08-20T03:15:42ZengElsevieriScience2589-00422025-07-0128711265810.1016/j.isci.2025.112658AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel diseaseLiying Lu0Keying Xu1Huiheng Qu2Lele Song3Yanan Song4Yanjun Wu5Xia Sun6Jingwen Kong7Qian Wen8Jiazheng Jiao9Zhanju Liu10Jiazeng Xia11Lixing Zhan12Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaDepartment of General Surgery, JiangNan University Medical Center, No. 68 Zhongshan Road, Liangxi District, Wuxi, Jiangsu Province 214000, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaDepartment of Gastroenterology, The Shanghai Tenth People’s Hospital, Tongji University, Shanghai 200072, ChinaDepartment of General Surgery, JiangNan University Medical Center, No. 68 Zhongshan Road, Liangxi District, Wuxi, Jiangsu Province 214000, ChinaShanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Corresponding authorSummary: The pathogenic factors of inflammatory bowel disease (IBD) are very complex, and further investigation of its pathogenesis is necessary. Some polar proteins have been reported to perform functions other than maintaining cell structure. In this study, we reported that the polar protein AF6 expressed by intestinal epithelial cells (IEC) can regulate the expression of MHCII in epithelial cells, thus affecting the cross dialogue between IECs and immune cells, and promoting the secretion of IgA by B cells to play a protective function. IgA supplementation can also improve the severe colitis phenotype caused by AF6 loss. Our study provides an understanding of the regulatory mechanisms of the intestinal epithelial expression of MHC II and its important role in colitis, which will aid in treatment and intervention.http://www.sciencedirect.com/science/article/pii/S2589004225009198ImmunologyCell biology
spellingShingle Liying Lu
Keying Xu
Huiheng Qu
Lele Song
Yanan Song
Yanjun Wu
Xia Sun
Jingwen Kong
Qian Wen
Jiazheng Jiao
Zhanju Liu
Jiazeng Xia
Lixing Zhan
AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease
iScience
Immunology
Cell biology
title AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease
title_full AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease
title_fullStr AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease
title_full_unstemmed AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease
title_short AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease
title_sort af6 regulates intestinal iga via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease
topic Immunology
Cell biology
url http://www.sciencedirect.com/science/article/pii/S2589004225009198
work_keys_str_mv AT liyinglu af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT keyingxu af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT huihengqu af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT lelesong af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT yanansong af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT yanjunwu af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT xiasun af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT jingwenkong af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT qianwen af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT jiazhengjiao af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT zhanjuliu af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT jiazengxia af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease
AT lixingzhan af6regulatesintestinaligaviacrosstalkbetweenintestinalepithelialcellsandimmunecellsininflammatoryboweldisease