Backflow reduction in local injection therapy with gelatin formulations
The local injection of therapeutic drugs, including cells, oncolytic viruses and nucleic acids, into different organs is an administrative route used to achieve high drug exposure at the site of action. However, after local injection, material backflow and side effect reactions can occur. Hence, thi...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/10717544.2024.2329100 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850138888623882240 |
|---|---|
| author | Kazuki Kotani Francois Marie Ngako Kadji Yoshinobu Mandai Yosuke Hiraoka |
| author_facet | Kazuki Kotani Francois Marie Ngako Kadji Yoshinobu Mandai Yosuke Hiraoka |
| author_sort | Kazuki Kotani |
| collection | DOAJ |
| description | The local injection of therapeutic drugs, including cells, oncolytic viruses and nucleic acids, into different organs is an administrative route used to achieve high drug exposure at the site of action. However, after local injection, material backflow and side effect reactions can occur. Hence, this study was carried out to investigate the effect of gelatin on backflow reduction in local injection. Gelatin particles (GPs) and hydrolyzed gelatin (HG) were injected into tissue models, including versatile training tissue (VTT), versatile training tissue tumor-in type (VTT-T), and broiler chicken muscles (BCM), using needle gauges between 23 G and 33 G. The backflow material fluid was collected with filter paper, and the backflow fluid rate was determined. The backflow rate was significantly reduced with 35 μm GPs (p value < .0001) at different concentrations up to 5% and with 75 μm GPs (p value < .01) up to 2% in the tissue models. The reduction in backflow with HG of different molecular weights showed that lower-molecular-weight HG required a higher-concentration dose (5% to 30%) and that higher-molecular-weight HG required a lower-concentration dose (7% to 8%). The backflow rate was significantly reduced with the gelatin-based formulation, in regard to the injection volumes, which varied from 10 μL to 100 μL with VTT or VTT-T and from 10 μL to 200 μL with BCM. The 35 μm GPs were injectable with needles of small gauges, which included 33 G, and the 75 μm GPs and HG were injectable with 27 G needles. The backflow rate was dependent on an optimal viscosity of the gelatin solutions. An optimal concentration of GPs or HG can prevent material backflow in local injection, and further studies with active drugs are necessary to investigate the applicability in tumor and organ injections. |
| format | Article |
| id | doaj-art-9a4ba86d248842bf882809c8cd2c23d5 |
| institution | OA Journals |
| issn | 1071-7544 1521-0464 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj-art-9a4ba86d248842bf882809c8cd2c23d52025-08-20T02:30:28ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642024-12-0131110.1080/10717544.2024.2329100Backflow reduction in local injection therapy with gelatin formulationsKazuki Kotani0Francois Marie Ngako Kadji1Yoshinobu Mandai2Yosuke Hiraoka3Department of Biomedical, R&D C-enter, Nitta Gelatin, Inc, Yao City, Osaka, JapanDepartment of Biomedical, R&D C-enter, Nitta Gelatin, Inc, Yao City, Osaka, JapanDepartment of Biomedical, R&D C-enter, Nitta Gelatin, Inc, Yao City, Osaka, JapanDepartment of Biomedical, R&D C-enter, Nitta Gelatin, Inc, Yao City, Osaka, JapanThe local injection of therapeutic drugs, including cells, oncolytic viruses and nucleic acids, into different organs is an administrative route used to achieve high drug exposure at the site of action. However, after local injection, material backflow and side effect reactions can occur. Hence, this study was carried out to investigate the effect of gelatin on backflow reduction in local injection. Gelatin particles (GPs) and hydrolyzed gelatin (HG) were injected into tissue models, including versatile training tissue (VTT), versatile training tissue tumor-in type (VTT-T), and broiler chicken muscles (BCM), using needle gauges between 23 G and 33 G. The backflow material fluid was collected with filter paper, and the backflow fluid rate was determined. The backflow rate was significantly reduced with 35 μm GPs (p value < .0001) at different concentrations up to 5% and with 75 μm GPs (p value < .01) up to 2% in the tissue models. The reduction in backflow with HG of different molecular weights showed that lower-molecular-weight HG required a higher-concentration dose (5% to 30%) and that higher-molecular-weight HG required a lower-concentration dose (7% to 8%). The backflow rate was significantly reduced with the gelatin-based formulation, in regard to the injection volumes, which varied from 10 μL to 100 μL with VTT or VTT-T and from 10 μL to 200 μL with BCM. The 35 μm GPs were injectable with needles of small gauges, which included 33 G, and the 75 μm GPs and HG were injectable with 27 G needles. The backflow rate was dependent on an optimal viscosity of the gelatin solutions. An optimal concentration of GPs or HG can prevent material backflow in local injection, and further studies with active drugs are necessary to investigate the applicability in tumor and organ injections.https://www.tandfonline.com/doi/10.1080/10717544.2024.2329100Local injectionbackflowgelatin particleshydrolyzed gelatin |
| spellingShingle | Kazuki Kotani Francois Marie Ngako Kadji Yoshinobu Mandai Yosuke Hiraoka Backflow reduction in local injection therapy with gelatin formulations Drug Delivery Local injection backflow gelatin particles hydrolyzed gelatin |
| title | Backflow reduction in local injection therapy with gelatin formulations |
| title_full | Backflow reduction in local injection therapy with gelatin formulations |
| title_fullStr | Backflow reduction in local injection therapy with gelatin formulations |
| title_full_unstemmed | Backflow reduction in local injection therapy with gelatin formulations |
| title_short | Backflow reduction in local injection therapy with gelatin formulations |
| title_sort | backflow reduction in local injection therapy with gelatin formulations |
| topic | Local injection backflow gelatin particles hydrolyzed gelatin |
| url | https://www.tandfonline.com/doi/10.1080/10717544.2024.2329100 |
| work_keys_str_mv | AT kazukikotani backflowreductioninlocalinjectiontherapywithgelatinformulations AT francoismariengakokadji backflowreductioninlocalinjectiontherapywithgelatinformulations AT yoshinobumandai backflowreductioninlocalinjectiontherapywithgelatinformulations AT yosukehiraoka backflowreductioninlocalinjectiontherapywithgelatinformulations |