Effect of Spinetoram Stress on Midgut Detoxification Enzyme and Gene Expression of <i>Apis cerana cerana</i> Fabricius
This study aimed to evaluate the impact of Spinetoram (SPI) on the midgut of <i>Apis cerana cerana</i> Fabricius pupae, emphasizing detoxifying enzyme activity, gene expression, and morphological alterations. Pupae were subjected to SPI at LC<sub>20</sub> and LC<sub>50&...
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2025-05-01
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| author | Lin Chen Tianjun He Linglong Ding Xinyan Lan Jiahao Sun Xiaoheng Xu Huafen Wu Dayun Zhou Zhichu Huang Tianxing Zhou Xiaoling Su Limin Chen |
| author_facet | Lin Chen Tianjun He Linglong Ding Xinyan Lan Jiahao Sun Xiaoheng Xu Huafen Wu Dayun Zhou Zhichu Huang Tianxing Zhou Xiaoling Su Limin Chen |
| author_sort | Lin Chen |
| collection | DOAJ |
| description | This study aimed to evaluate the impact of Spinetoram (SPI) on the midgut of <i>Apis cerana cerana</i> Fabricius pupae, emphasizing detoxifying enzyme activity, gene expression, and morphological alterations. Pupae were subjected to SPI at LC<sub>20</sub> and LC<sub>50</sub> concentrations, and the midgut was evaluated using morphological assessment, detoxifying enzyme assays, and transcriptome analysis utilizing gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Transcriptome analysis revealed 32 differentially expressed genes (DEGs) that were common to both the LC<sub>20</sub> vs. control (CK) and LC<sub>50</sub> vs. CK comparisons, along with 24 DEGs unique to the LC<sub>20</sub> vs. CK comparison and 76 DEGs unique to the LC<sub>50</sub> vs. CK comparison. KEGG pathway analysis indicated the substantial enrichment of pathways associated with drug metabolism, xenobiotic metabolism, and amino acid metabolism, implying disruptions in detoxification mechanisms and broader metabolic imbalances resulting from SPI exposure. Morphological analysis showed a normal midgut structure in the control group, while significant damage was observed in the LC<sub>20</sub> group, and severe degeneration was observed in the LC<sub>50</sub> group. Detoxification enzyme assays revealed that the activities of cytochrome P450, glutathione S-transferase, and carboxylesterase significantly increased at LC<sub>20</sub> (<i>p</i> < 0.05), indicating an initial induction of detoxification responses; however, they declined at LC<sub>50</sub>, suggesting enzyme inhibition or midgut damage. The activity of acetylcholinesterase markedly diminished at both LC<sub>20</sub> and LC<sub>50</sub> (<i>p</i> < 0.05), with a more substantial decline observed at LC<sub>50</sub>, suggesting possible neurotoxicity. These findings indicate that SPI exposure causes substantial alterations in midgut morphology, detoxifying enzyme activity, and gene expression in <i>Apis cerana cerana</i> Fabricius pupae, underscoring the insecticide’s detrimental impact on honey bee health. |
| format | Article |
| id | doaj-art-9a468d6a59fd4e4a886883a5b8524762 |
| institution | OA Journals |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Insects |
| spelling | doaj-art-9a468d6a59fd4e4a886883a5b85247622025-08-20T02:34:00ZengMDPI AGInsects2075-44502025-05-0116549210.3390/insects16050492Effect of Spinetoram Stress on Midgut Detoxification Enzyme and Gene Expression of <i>Apis cerana cerana</i> FabriciusLin Chen0Tianjun He1Linglong Ding2Xinyan Lan3Jiahao Sun4Xiaoheng Xu5Huafen Wu6Dayun Zhou7Zhichu Huang8Tianxing Zhou9Xiaoling Su10Limin Chen11Lishui Institute of Agriculture and Forestry Sciences, Lishui 323000, ChinaLishui Institute of Agriculture and Forestry Sciences, Lishui 323000, ChinaCollege of Ecology, Lishui University, Lishui 323000, ChinaCollege of Ecology, Lishui University, Lishui 323000, ChinaLishui Institute of Agriculture and Forestry Sciences, Lishui 323000, ChinaLishui Institute of Agriculture and Forestry Sciences, Lishui 323000, ChinaLishui Institute of Agriculture and Forestry Sciences, Lishui 323000, ChinaLishui Institute of Agriculture and Forestry Sciences, Lishui 323000, ChinaJinhua Academy of Agricultural Sciences, Jinhua 321000, ChinaLishui Institute of Agriculture and Forestry Sciences, Lishui 323000, ChinaJinhua Academy of Agricultural Sciences, Jinhua 321000, ChinaLishui Institute of Agriculture and Forestry Sciences, Lishui 323000, ChinaThis study aimed to evaluate the impact of Spinetoram (SPI) on the midgut of <i>Apis cerana cerana</i> Fabricius pupae, emphasizing detoxifying enzyme activity, gene expression, and morphological alterations. Pupae were subjected to SPI at LC<sub>20</sub> and LC<sub>50</sub> concentrations, and the midgut was evaluated using morphological assessment, detoxifying enzyme assays, and transcriptome analysis utilizing gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Transcriptome analysis revealed 32 differentially expressed genes (DEGs) that were common to both the LC<sub>20</sub> vs. control (CK) and LC<sub>50</sub> vs. CK comparisons, along with 24 DEGs unique to the LC<sub>20</sub> vs. CK comparison and 76 DEGs unique to the LC<sub>50</sub> vs. CK comparison. KEGG pathway analysis indicated the substantial enrichment of pathways associated with drug metabolism, xenobiotic metabolism, and amino acid metabolism, implying disruptions in detoxification mechanisms and broader metabolic imbalances resulting from SPI exposure. Morphological analysis showed a normal midgut structure in the control group, while significant damage was observed in the LC<sub>20</sub> group, and severe degeneration was observed in the LC<sub>50</sub> group. Detoxification enzyme assays revealed that the activities of cytochrome P450, glutathione S-transferase, and carboxylesterase significantly increased at LC<sub>20</sub> (<i>p</i> < 0.05), indicating an initial induction of detoxification responses; however, they declined at LC<sub>50</sub>, suggesting enzyme inhibition or midgut damage. The activity of acetylcholinesterase markedly diminished at both LC<sub>20</sub> and LC<sub>50</sub> (<i>p</i> < 0.05), with a more substantial decline observed at LC<sub>50</sub>, suggesting possible neurotoxicity. These findings indicate that SPI exposure causes substantial alterations in midgut morphology, detoxifying enzyme activity, and gene expression in <i>Apis cerana cerana</i> Fabricius pupae, underscoring the insecticide’s detrimental impact on honey bee health.https://www.mdpi.com/2075-4450/16/5/492SPI toxicityhoney beemidgut damageenzyme inhibition |
| spellingShingle | Lin Chen Tianjun He Linglong Ding Xinyan Lan Jiahao Sun Xiaoheng Xu Huafen Wu Dayun Zhou Zhichu Huang Tianxing Zhou Xiaoling Su Limin Chen Effect of Spinetoram Stress on Midgut Detoxification Enzyme and Gene Expression of <i>Apis cerana cerana</i> Fabricius Insects SPI toxicity honey bee midgut damage enzyme inhibition |
| title | Effect of Spinetoram Stress on Midgut Detoxification Enzyme and Gene Expression of <i>Apis cerana cerana</i> Fabricius |
| title_full | Effect of Spinetoram Stress on Midgut Detoxification Enzyme and Gene Expression of <i>Apis cerana cerana</i> Fabricius |
| title_fullStr | Effect of Spinetoram Stress on Midgut Detoxification Enzyme and Gene Expression of <i>Apis cerana cerana</i> Fabricius |
| title_full_unstemmed | Effect of Spinetoram Stress on Midgut Detoxification Enzyme and Gene Expression of <i>Apis cerana cerana</i> Fabricius |
| title_short | Effect of Spinetoram Stress on Midgut Detoxification Enzyme and Gene Expression of <i>Apis cerana cerana</i> Fabricius |
| title_sort | effect of spinetoram stress on midgut detoxification enzyme and gene expression of i apis cerana cerana i fabricius |
| topic | SPI toxicity honey bee midgut damage enzyme inhibition |
| url | https://www.mdpi.com/2075-4450/16/5/492 |
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