Embryonic toxicology evaluation of novel Cissus quadrangularis, bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish model
Introduction: The development of novel scaffold incorporating Cissus quadrangularis extract, bioceramics and tendon extracellular matrix (ECM) for periodontal bone regeneration necessitates a thorough assessment of their embryotoxicity to ensure biocompatibility and safety. This study evaluates the...
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| Format: | Article |
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Elsevier
2025-05-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825000727 |
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| author | S. Balaji Ganesh Manoj Aravindan Gurumoorthy Kaarthikeyan Taniya Mary Martin Meenakshi Sundaram Kishore Kumar S. Chitra |
| author_facet | S. Balaji Ganesh Manoj Aravindan Gurumoorthy Kaarthikeyan Taniya Mary Martin Meenakshi Sundaram Kishore Kumar S. Chitra |
| author_sort | S. Balaji Ganesh |
| collection | DOAJ |
| description | Introduction: The development of novel scaffold incorporating Cissus quadrangularis extract, bioceramics and tendon extracellular matrix (ECM) for periodontal bone regeneration necessitates a thorough assessment of their embryotoxicity to ensure biocompatibility and safety. This study evaluates the embryonic toxicology of these innovative scaffold using the zebrafish model, which provides a rapid transparent and highly sensitive system for assessing development toxicity. Materials and methods: Zebrafish embryos were exposed to scaffold containing Cissus quadrangularis extract (80 % ethanol), bioceramics, and tendon extracellular. Briefly, the scaffold immerged in the E3 medium for 48h and the extract (10, 50 and 100μl/ml) was evaluated against developing embryos for different developmental anomalies such as survival, malformation, heartbeat and the expression of RunX2 and Bmp2 genes. Results: The results showed that the SEM analysis revealed that the membrane was rough in nature. FTIR analysis confirmed the presence of hydroxylate groups, collagen and hydroxyapatite in the synthesized membrane. Meanwhile, the scaffold did not show any of the developmental defects such as hatchability inhibition and neural toxicity. The mortality was comparable to negative control. Further, the study revealed that the scaffold induced the osteogenic potential by elevating the RunX2 and Bmp2 expression. Conclusion: This study highlighted the inclusion of C. quadrangularis extract could be a beneficial for enhancing periodontal bone regeneration. Further, this also revealed that the extract did not impede the normal tissue development or regeneration using zebrafish embryo as a model. |
| format | Article |
| id | doaj-art-9a324e99df584fc093174bda416f8a42 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-9a324e99df584fc093174bda416f8a422025-08-20T03:49:33ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-05-0115356356910.1016/j.jobcr.2025.03.009Embryonic toxicology evaluation of novel Cissus quadrangularis, bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish modelS. Balaji Ganesh0Manoj Aravindan1Gurumoorthy Kaarthikeyan2Taniya Mary Martin3Meenakshi Sundaram Kishore Kumar4S. Chitra5Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, Tamil Nadu, India; Corresponding author.Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, Tamil Nadu, IndiaDepartment of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, Tamil Nadu, IndiaDepartment of Anatomy, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, Tamil Nadu, IndiaDepartment of Anatomy, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, Tamil Nadu, IndiaDepartment of Biomaterials, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, Tamil Nadu, IndiaIntroduction: The development of novel scaffold incorporating Cissus quadrangularis extract, bioceramics and tendon extracellular matrix (ECM) for periodontal bone regeneration necessitates a thorough assessment of their embryotoxicity to ensure biocompatibility and safety. This study evaluates the embryonic toxicology of these innovative scaffold using the zebrafish model, which provides a rapid transparent and highly sensitive system for assessing development toxicity. Materials and methods: Zebrafish embryos were exposed to scaffold containing Cissus quadrangularis extract (80 % ethanol), bioceramics, and tendon extracellular. Briefly, the scaffold immerged in the E3 medium for 48h and the extract (10, 50 and 100μl/ml) was evaluated against developing embryos for different developmental anomalies such as survival, malformation, heartbeat and the expression of RunX2 and Bmp2 genes. Results: The results showed that the SEM analysis revealed that the membrane was rough in nature. FTIR analysis confirmed the presence of hydroxylate groups, collagen and hydroxyapatite in the synthesized membrane. Meanwhile, the scaffold did not show any of the developmental defects such as hatchability inhibition and neural toxicity. The mortality was comparable to negative control. Further, the study revealed that the scaffold induced the osteogenic potential by elevating the RunX2 and Bmp2 expression. Conclusion: This study highlighted the inclusion of C. quadrangularis extract could be a beneficial for enhancing periodontal bone regeneration. Further, this also revealed that the extract did not impede the normal tissue development or regeneration using zebrafish embryo as a model.http://www.sciencedirect.com/science/article/pii/S2212426825000727Embryonic toxicologyCissus quadrangularis extractBioceramicsTendon ECMZebrafish embryo |
| spellingShingle | S. Balaji Ganesh Manoj Aravindan Gurumoorthy Kaarthikeyan Taniya Mary Martin Meenakshi Sundaram Kishore Kumar S. Chitra Embryonic toxicology evaluation of novel Cissus quadrangularis, bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish model Journal of Oral Biology and Craniofacial Research Embryonic toxicology Cissus quadrangularis extract Bioceramics Tendon ECM Zebrafish embryo |
| title | Embryonic toxicology evaluation of novel Cissus quadrangularis, bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish model |
| title_full | Embryonic toxicology evaluation of novel Cissus quadrangularis, bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish model |
| title_fullStr | Embryonic toxicology evaluation of novel Cissus quadrangularis, bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish model |
| title_full_unstemmed | Embryonic toxicology evaluation of novel Cissus quadrangularis, bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish model |
| title_short | Embryonic toxicology evaluation of novel Cissus quadrangularis, bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish model |
| title_sort | embryonic toxicology evaluation of novel cissus quadrangularis bioceramics and tendon extracellular matrix incorporated scaffolds for periodontal bone regeneration using zebrafish model |
| topic | Embryonic toxicology Cissus quadrangularis extract Bioceramics Tendon ECM Zebrafish embryo |
| url | http://www.sciencedirect.com/science/article/pii/S2212426825000727 |
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