Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease
The involvement of the intestinal microbiome in the pathogenesis of inflammatory bowel disease (IBD) and colorectal cancer (CRC), is well-established. Bacteria interact with immune cells at sites of intestinal inflammation, but also in the CRC tumor microenvironment. We hypothesized that bacterial r...
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2025.2530157 |
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| author | Yasser Morsy Åsa Walberg Paulina Wawrzyniak Barbara Hubeli Luca Truscello Celine Mamie Ania Niechcial Emilie Gueguen Roberto Manzini Claudia Gottier Silvia Lang Sylvie Scharl Sena Blümel Luc Biedermann Gerhard Rogler Matthias Turina Michaela Ramser Henrik Petrowsky Isabelle C. Arnold Sebastian Zeissig Nicola Zamboni Adrian Egli Jan Hendrik Niess Petr Hruz Alexander Knuth Ralph Fritsch Markus G. Manz Marcin Wawrzyniak Michael Scharl |
| author_facet | Yasser Morsy Åsa Walberg Paulina Wawrzyniak Barbara Hubeli Luca Truscello Celine Mamie Ania Niechcial Emilie Gueguen Roberto Manzini Claudia Gottier Silvia Lang Sylvie Scharl Sena Blümel Luc Biedermann Gerhard Rogler Matthias Turina Michaela Ramser Henrik Petrowsky Isabelle C. Arnold Sebastian Zeissig Nicola Zamboni Adrian Egli Jan Hendrik Niess Petr Hruz Alexander Knuth Ralph Fritsch Markus G. Manz Marcin Wawrzyniak Michael Scharl |
| author_sort | Yasser Morsy |
| collection | DOAJ |
| description | The involvement of the intestinal microbiome in the pathogenesis of inflammatory bowel disease (IBD) and colorectal cancer (CRC), is well-established. Bacteria interact with immune cells at sites of intestinal inflammation, but also in the CRC tumor microenvironment. We hypothesized that bacterial remnants translocate within peripheral blood mononuclear cells (PBMCs) into the circulation and thus explored the composition of the detectable microbiome in PBMCs of patients with CRC or IBD compared to healthy controls. The PBMC microbiome profiles partially align with the tumor-derived or intestinal tissue-derived microbiome signatures obtained from the same patients with CRC or IBD, respectively. Our metagenomics data, supported by 16S-rRNA-FISH-Flow, imaging flow cytometry and species-specific qPCR, revealed the presence of translocated bacterial genetic sequences in the patients with CRC and IBD. Thus, our data suggest that in patients with intestinal barrier leakage, there is the potential for the translocation of bacterial remnants into the circulation via PBMCs. |
| format | Article |
| id | doaj-art-9a2ead003fb346f1bff93ddf5ee84d71 |
| institution | Kabale University |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-9a2ead003fb346f1bff93ddf5ee84d712025-08-20T03:28:13ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2025.2530157Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel diseaseYasser Morsy0Åsa Walberg1Paulina Wawrzyniak2Barbara Hubeli3Luca Truscello4Celine Mamie5Ania Niechcial6Emilie Gueguen7Roberto Manzini8Claudia Gottier9Silvia Lang10Sylvie Scharl11Sena Blümel12Luc Biedermann13Gerhard Rogler14Matthias Turina15Michaela Ramser16Henrik Petrowsky17Isabelle C. Arnold18Sebastian Zeissig19Nicola Zamboni20Adrian Egli21Jan Hendrik Niess22Petr Hruz23Alexander Knuth24Ralph Fritsch25Markus G. Manz26Marcin Wawrzyniak27Michael Scharl28Department of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Visceral and Transplant Surgery, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Visceral and Transplant Surgery, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Visceral and Transplant Surgery, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandInstitute of Experimental Immunology, University of Zürich, Zurich, SwitzerlandCenter for Regenerative Therapies Dresden (CRTD), Technische Universität (TU) Dresden, and Department of Medicine I, University Medical Center Dresden, Dresden, GermanyInstitute of Molecular Systems Biology, Federal Institute of Technology Zurich, Zurich, SwitzerlandInstitute of Medical Microbiology, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Digestive Healthcare Center, Basel, SwitzerlandDepartment of Gastroenterology and Hepatology, University Digestive Healthcare Center, Basel, SwitzerlandDepartment of Medical Oncology and Hematology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Medical Oncology and Hematology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Medical Oncology and Hematology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, Zurich, SwitzerlandThe involvement of the intestinal microbiome in the pathogenesis of inflammatory bowel disease (IBD) and colorectal cancer (CRC), is well-established. Bacteria interact with immune cells at sites of intestinal inflammation, but also in the CRC tumor microenvironment. We hypothesized that bacterial remnants translocate within peripheral blood mononuclear cells (PBMCs) into the circulation and thus explored the composition of the detectable microbiome in PBMCs of patients with CRC or IBD compared to healthy controls. The PBMC microbiome profiles partially align with the tumor-derived or intestinal tissue-derived microbiome signatures obtained from the same patients with CRC or IBD, respectively. Our metagenomics data, supported by 16S-rRNA-FISH-Flow, imaging flow cytometry and species-specific qPCR, revealed the presence of translocated bacterial genetic sequences in the patients with CRC and IBD. Thus, our data suggest that in patients with intestinal barrier leakage, there is the potential for the translocation of bacterial remnants into the circulation via PBMCs.https://www.tandfonline.com/doi/10.1080/19490976.2025.2530157Microbiomecolorectal cancer metastasisinflammatory bowel disease pathogenesiscancer and microbiomeintestinal epithelial barrier defectperipheral blood mononuclear cells |
| spellingShingle | Yasser Morsy Åsa Walberg Paulina Wawrzyniak Barbara Hubeli Luca Truscello Celine Mamie Ania Niechcial Emilie Gueguen Roberto Manzini Claudia Gottier Silvia Lang Sylvie Scharl Sena Blümel Luc Biedermann Gerhard Rogler Matthias Turina Michaela Ramser Henrik Petrowsky Isabelle C. Arnold Sebastian Zeissig Nicola Zamboni Adrian Egli Jan Hendrik Niess Petr Hruz Alexander Knuth Ralph Fritsch Markus G. Manz Marcin Wawrzyniak Michael Scharl Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease Gut Microbes Microbiome colorectal cancer metastasis inflammatory bowel disease pathogenesis cancer and microbiome intestinal epithelial barrier defect peripheral blood mononuclear cells |
| title | Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease |
| title_full | Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease |
| title_fullStr | Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease |
| title_full_unstemmed | Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease |
| title_short | Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease |
| title_sort | blood borne immune cells carry low biomass dna remnants of microbes in patients with colorectal cancer or inflammatory bowel disease |
| topic | Microbiome colorectal cancer metastasis inflammatory bowel disease pathogenesis cancer and microbiome intestinal epithelial barrier defect peripheral blood mononuclear cells |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2025.2530157 |
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