Drug Resistance of Mycobacterium tuberculosis Based on Whole-Genome Sequencing in the Yi Ethnic Group, Sichuan Province, China
This study investigated drug-resistant tuberculosis (DR-TB) in the Yi ethnic group. The study was designed to identify risk factors for DR-TB and its relationship with HIV/AIDS. To establish the resistance to antituberculosis drugs, whole-genome sequencing (WGS) was performed using culture-positive...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2023/4431209 |
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| author | Wenfeng Gao Xiaoru Chen Liang Yao Jing Li Yuan Gao Ting Li Yunkui Li Weina Wang Shu Zhang Jinge He |
| author_facet | Wenfeng Gao Xiaoru Chen Liang Yao Jing Li Yuan Gao Ting Li Yunkui Li Weina Wang Shu Zhang Jinge He |
| author_sort | Wenfeng Gao |
| collection | DOAJ |
| description | This study investigated drug-resistant tuberculosis (DR-TB) in the Yi ethnic group. The study was designed to identify risk factors for DR-TB and its relationship with HIV/AIDS. To establish the resistance to antituberculosis drugs, whole-genome sequencing (WGS) was performed using culture-positive Mycobacterium tuberculosis samples collected from people of the Yi ethnic group from March 2019 to March 2021. Baseline characteristics were obtained from China’s tuberculosis surveillance system. A total of 116 M. tuberculosis strains were included in the final analysis. Lineage 2.2 (75.86%) was the dominant sublineage, followed by lineage 4.5 (18.97%) and lineage 4.4 (5.17%). The rates of rifampicin-resistant (RR-TB), multidrug-resistant (MDR-TB), and preextensively drug-resistant TB (pre-XDR-TB) were 18.97%, 10.34%, and 6.03%, respectively. Drug-resistant strains were not found in the elderly (age≥65 years). The proportions of RR/MDR-TB and pre-XDR-TB cases among re-treatment patients were higher than those among new patients (χ2=12.155, P=0.003; χ2=22.495, P=0.001, respectively). The pre-XDR-TB case proportions were higher among female patients than among males and higher among referred patients (χ2=5.456, P=0.032; χ2=15.134, P=0.002, respectively). The rates of RR/MDR-TB and pre-XDR-TB did not differ appreciably among groups with different HIV infection statuses nor lineage populations. DR-TB poses a serious challenge to the Yi ethnic group. Re-treatment patients, women, and referred patients were at high risk of MDR/RR-TB or pre-XDR-TB while HIV and lineage 2 had negligible association with drug resistance. Whole-genome sequencing should be used to guide the design of treatment regimens and to tailor public interventions. |
| format | Article |
| id | doaj-art-9a29c9b813634e3a8a4e6576b8c246a8 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9a29c9b813634e3a8a4e6576b8c246a82025-08-20T03:36:54ZengWileyJournal of Immunology Research2314-71562023-01-01202310.1155/2023/4431209Drug Resistance of Mycobacterium tuberculosis Based on Whole-Genome Sequencing in the Yi Ethnic Group, Sichuan Province, ChinaWenfeng Gao0Xiaoru Chen1Liang Yao2Jing Li3Yuan Gao4Ting Li5Yunkui Li6Weina Wang7Shu Zhang8Jinge He9Institute for Tuberculosis Control and PreventionLaboratory MedicineLaboratory MedicineInstitute for Tuberculosis Control and PreventionInstitute for Tuberculosis Control and PreventionInstitute for Tuberculosis Control and PreventionInstitute for Tuberculosis Control and PreventionInstitute for Tuberculosis Control and PreventionInstitute for Tuberculosis Control and PreventionInstitute for Tuberculosis Control and PreventionThis study investigated drug-resistant tuberculosis (DR-TB) in the Yi ethnic group. The study was designed to identify risk factors for DR-TB and its relationship with HIV/AIDS. To establish the resistance to antituberculosis drugs, whole-genome sequencing (WGS) was performed using culture-positive Mycobacterium tuberculosis samples collected from people of the Yi ethnic group from March 2019 to March 2021. Baseline characteristics were obtained from China’s tuberculosis surveillance system. A total of 116 M. tuberculosis strains were included in the final analysis. Lineage 2.2 (75.86%) was the dominant sublineage, followed by lineage 4.5 (18.97%) and lineage 4.4 (5.17%). The rates of rifampicin-resistant (RR-TB), multidrug-resistant (MDR-TB), and preextensively drug-resistant TB (pre-XDR-TB) were 18.97%, 10.34%, and 6.03%, respectively. Drug-resistant strains were not found in the elderly (age≥65 years). The proportions of RR/MDR-TB and pre-XDR-TB cases among re-treatment patients were higher than those among new patients (χ2=12.155, P=0.003; χ2=22.495, P=0.001, respectively). The pre-XDR-TB case proportions were higher among female patients than among males and higher among referred patients (χ2=5.456, P=0.032; χ2=15.134, P=0.002, respectively). The rates of RR/MDR-TB and pre-XDR-TB did not differ appreciably among groups with different HIV infection statuses nor lineage populations. DR-TB poses a serious challenge to the Yi ethnic group. Re-treatment patients, women, and referred patients were at high risk of MDR/RR-TB or pre-XDR-TB while HIV and lineage 2 had negligible association with drug resistance. Whole-genome sequencing should be used to guide the design of treatment regimens and to tailor public interventions.http://dx.doi.org/10.1155/2023/4431209 |
| spellingShingle | Wenfeng Gao Xiaoru Chen Liang Yao Jing Li Yuan Gao Ting Li Yunkui Li Weina Wang Shu Zhang Jinge He Drug Resistance of Mycobacterium tuberculosis Based on Whole-Genome Sequencing in the Yi Ethnic Group, Sichuan Province, China Journal of Immunology Research |
| title | Drug Resistance of Mycobacterium tuberculosis Based on Whole-Genome Sequencing in the Yi Ethnic Group, Sichuan Province, China |
| title_full | Drug Resistance of Mycobacterium tuberculosis Based on Whole-Genome Sequencing in the Yi Ethnic Group, Sichuan Province, China |
| title_fullStr | Drug Resistance of Mycobacterium tuberculosis Based on Whole-Genome Sequencing in the Yi Ethnic Group, Sichuan Province, China |
| title_full_unstemmed | Drug Resistance of Mycobacterium tuberculosis Based on Whole-Genome Sequencing in the Yi Ethnic Group, Sichuan Province, China |
| title_short | Drug Resistance of Mycobacterium tuberculosis Based on Whole-Genome Sequencing in the Yi Ethnic Group, Sichuan Province, China |
| title_sort | drug resistance of mycobacterium tuberculosis based on whole genome sequencing in the yi ethnic group sichuan province china |
| url | http://dx.doi.org/10.1155/2023/4431209 |
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