The Relationships between Anti-Thyroid Peroxidase (Anti-TPO) and Inhibin-B as Predictor Biomarkers for Female Infertility
Anti-thyroid peroxidase is an autoantibody generated by the immune system, its primary function is target and neutralize the enzyme thyroid peroxidase, is essential for production of thyroid hormones. Inhibin-B is a peptide belonging to TGF-β family produced by granulosa cells of the growing follic...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2025-07-01
|
| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/60150 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850106163665829888 |
|---|---|
| author | Zahraa Abd Al-Amir Jalil Mamitha Abeer Cheaid Yousif Al-Fatlawi Manal Nasih Ahmed Hamdan Al-Tamimi |
| author_facet | Zahraa Abd Al-Amir Jalil Mamitha Abeer Cheaid Yousif Al-Fatlawi Manal Nasih Ahmed Hamdan Al-Tamimi |
| author_sort | Zahraa Abd Al-Amir Jalil Mamitha |
| collection | DOAJ |
| description |
Anti-thyroid peroxidase is an autoantibody generated by the immune system, its primary function is target and neutralize the enzyme thyroid peroxidase, is essential for production of thyroid hormones. Inhibin-B is a peptide belonging to TGF-β family produced by granulosa cells of the growing follicle cohort. It reflects the health of follicles, their overall numbers. The objective of this article is evaluation of Anti-TPO, Inhibin-B for female infertility. A case-control study includes 120females between 20-45 years divided into 60 apparently healthy females used as controls and 60 infertile females distributed 37 with Polycystic ovarian syndrome, 23 with Premature ovarian failure. Serum levels of Anti-TPO, Inhibin-B, LH, FSH, Testosterone, Prolactin and Estrogen were all measured using an ELISA assay. Inhibin-B, Anti-TPO higher in patients than in controls. Inhibin-B significantly increases(p≤0.0001) in PCOS above and under 40years (273.55±75.25 pg/mL), (234.91±52.43 pg/mL), but decreases in POF (18.96±1.21 pg/mL) than in controls (35.58±10.48 pg/mL), (42.04±10.04 pg/mL). AntiTPO increases in PCOS and POF above and under 40 years (16.08±2.14 ng/mL), (17.63±1.93 ng/mL), (14.72±1.19 ng/mL) than in controls(5.92±0.99 ng/mL), (5.93±0.84 ng/mL). The levels of FSH, Prolactin, Testosterone, Estrogen and LH were increased (p≤0.0001) in PCOS, POF as in controls. The results showed a negative correlation (p≤0.0001) between Inhibin-B, Anti-TPO (r = -0.520) in PCOS. The Receiver operating characteristic (ROC) analysis demonstrated Inhibin-B 95%CI:0.907-1.000; P-value:0.001; Cutoff Point:77.347; AUC:95.417% with Sensitivity to Specificity 91.667-98.333%; Accuracy: 95.000%. Anti-TPO 95% CI: 0.907-1.000; P-value: 0.001; Cutoff Point: 9.306; AUC: 98.861% with Sensitivity to Specificity 96.676-96.667%; Accuracy: 97.000%. Anti-TPO, Inhibin-B are useful biomarkers for evaluating fertility status and ovarian health, can used as sensitive indicators for early diagnosis of PCOS and POF in women of reproductive age.
|
| format | Article |
| id | doaj-art-9a24c843cb8b43bfbc8f33c22d63eebf |
| institution | OA Journals |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-9a24c843cb8b43bfbc8f33c22d63eebf2025-08-20T02:38:53ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942025-07-0132610.4308/hjb.32.6.1415-1425The Relationships between Anti-Thyroid Peroxidase (Anti-TPO) and Inhibin-B as Predictor Biomarkers for Female InfertilityZahraa Abd Al-Amir Jalil Mamitha0Abeer Cheaid Yousif Al-Fatlawi1Manal Nasih Ahmed Hamdan Al-Tamimi2Department of Clinical Laboratories, College of Applied Medical Science, University of Kerbala, Kerbala, IraqDepartment of Clinical Laboratories, College of Applied Medical Science, University of Kerbala, Kerbala, IraqCollege of Medicine, University of Kerbala, Kerbala, Iraq Anti-thyroid peroxidase is an autoantibody generated by the immune system, its primary function is target and neutralize the enzyme thyroid peroxidase, is essential for production of thyroid hormones. Inhibin-B is a peptide belonging to TGF-β family produced by granulosa cells of the growing follicle cohort. It reflects the health of follicles, their overall numbers. The objective of this article is evaluation of Anti-TPO, Inhibin-B for female infertility. A case-control study includes 120females between 20-45 years divided into 60 apparently healthy females used as controls and 60 infertile females distributed 37 with Polycystic ovarian syndrome, 23 with Premature ovarian failure. Serum levels of Anti-TPO, Inhibin-B, LH, FSH, Testosterone, Prolactin and Estrogen were all measured using an ELISA assay. Inhibin-B, Anti-TPO higher in patients than in controls. Inhibin-B significantly increases(p≤0.0001) in PCOS above and under 40years (273.55±75.25 pg/mL), (234.91±52.43 pg/mL), but decreases in POF (18.96±1.21 pg/mL) than in controls (35.58±10.48 pg/mL), (42.04±10.04 pg/mL). AntiTPO increases in PCOS and POF above and under 40 years (16.08±2.14 ng/mL), (17.63±1.93 ng/mL), (14.72±1.19 ng/mL) than in controls(5.92±0.99 ng/mL), (5.93±0.84 ng/mL). The levels of FSH, Prolactin, Testosterone, Estrogen and LH were increased (p≤0.0001) in PCOS, POF as in controls. The results showed a negative correlation (p≤0.0001) between Inhibin-B, Anti-TPO (r = -0.520) in PCOS. The Receiver operating characteristic (ROC) analysis demonstrated Inhibin-B 95%CI:0.907-1.000; P-value:0.001; Cutoff Point:77.347; AUC:95.417% with Sensitivity to Specificity 91.667-98.333%; Accuracy: 95.000%. Anti-TPO 95% CI: 0.907-1.000; P-value: 0.001; Cutoff Point: 9.306; AUC: 98.861% with Sensitivity to Specificity 96.676-96.667%; Accuracy: 97.000%. Anti-TPO, Inhibin-B are useful biomarkers for evaluating fertility status and ovarian health, can used as sensitive indicators for early diagnosis of PCOS and POF in women of reproductive age. https://journal.ipb.ac.id/index.php/hayati/article/view/60150 |
| spellingShingle | Zahraa Abd Al-Amir Jalil Mamitha Abeer Cheaid Yousif Al-Fatlawi Manal Nasih Ahmed Hamdan Al-Tamimi The Relationships between Anti-Thyroid Peroxidase (Anti-TPO) and Inhibin-B as Predictor Biomarkers for Female Infertility Hayati Journal of Biosciences |
| title | The Relationships between Anti-Thyroid Peroxidase (Anti-TPO) and Inhibin-B as Predictor Biomarkers for Female Infertility |
| title_full | The Relationships between Anti-Thyroid Peroxidase (Anti-TPO) and Inhibin-B as Predictor Biomarkers for Female Infertility |
| title_fullStr | The Relationships between Anti-Thyroid Peroxidase (Anti-TPO) and Inhibin-B as Predictor Biomarkers for Female Infertility |
| title_full_unstemmed | The Relationships between Anti-Thyroid Peroxidase (Anti-TPO) and Inhibin-B as Predictor Biomarkers for Female Infertility |
| title_short | The Relationships between Anti-Thyroid Peroxidase (Anti-TPO) and Inhibin-B as Predictor Biomarkers for Female Infertility |
| title_sort | relationships between anti thyroid peroxidase anti tpo and inhibin b as predictor biomarkers for female infertility |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/60150 |
| work_keys_str_mv | AT zahraaabdalamirjalilmamitha therelationshipsbetweenantithyroidperoxidaseantitpoandinhibinbaspredictorbiomarkersforfemaleinfertility AT abeercheaidyousifalfatlawi therelationshipsbetweenantithyroidperoxidaseantitpoandinhibinbaspredictorbiomarkersforfemaleinfertility AT manalnasihahmedhamdanaltamimi therelationshipsbetweenantithyroidperoxidaseantitpoandinhibinbaspredictorbiomarkersforfemaleinfertility AT zahraaabdalamirjalilmamitha relationshipsbetweenantithyroidperoxidaseantitpoandinhibinbaspredictorbiomarkersforfemaleinfertility AT abeercheaidyousifalfatlawi relationshipsbetweenantithyroidperoxidaseantitpoandinhibinbaspredictorbiomarkersforfemaleinfertility AT manalnasihahmedhamdanaltamimi relationshipsbetweenantithyroidperoxidaseantitpoandinhibinbaspredictorbiomarkersforfemaleinfertility |