Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling
Recent studies have indicated that the Zika virus (ZIKV) has a significant impact on the fetal brain, and autophagy is contributing to host immune response and defense against virus infection. Here, we demonstrate that ZIKV infection triggered increased LC3 punctuation in mouse monocyte-macrophage c...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/9527147 |
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| author | Yuyi Huang Yujie Wang Shuhui Meng Zhuohang Chen Haifan Kong Ting Pan Gen Lu Xuefeng Li |
| author_facet | Yuyi Huang Yujie Wang Shuhui Meng Zhuohang Chen Haifan Kong Ting Pan Gen Lu Xuefeng Li |
| author_sort | Yuyi Huang |
| collection | DOAJ |
| description | Recent studies have indicated that the Zika virus (ZIKV) has a significant impact on the fetal brain, and autophagy is contributing to host immune response and defense against virus infection. Here, we demonstrate that ZIKV infection triggered increased LC3 punctuation in mouse monocyte-macrophage cell line (RAW264.7), mouse microglial cell line (BV2), and hindbrain tissues, proving the occurrence of autophagy both in vitro and in vivo. Interestingly, manual intervention of autophagy, like deficiency inhibited by 3-MA, can reduce viral clearance in RAW264.7 cells upon ZIKV infection. Besides, specific siRNA strategy confirmed that autophagy can be activated through Atg7-Atg5 and type I IFN signaling pathway upon ZIKV infection, while knocking down of Atg7 and Atg5 effectively decreased the ZIKV clearance in phagocytes. Furthermore, we analyzed that type I IFN signaling could contribute to autophagic clearance of invaded ZIKV in phagocytes. Taken together, our findings demonstrate that ZIKV-induced autophagy is favorable to activate host immunity, particularly through type I IFN signaling, which participates in host protection and defense against ZIKV infection. |
| format | Article |
| id | doaj-art-9a1cf6c2511941a3a112a9e64734374c |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-9a1cf6c2511941a3a112a9e64734374c2025-02-03T05:44:15ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/95271479527147Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN SignalingYuyi Huang0Yujie Wang1Shuhui Meng2Zhuohang Chen3Haifan Kong4Ting Pan5Gen Lu6Xuefeng Li7The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital; The Second Affiliated Hospital of Guangzhou Medical University, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology; Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, ChinaThe Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital; The Second Affiliated Hospital of Guangzhou Medical University, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology; Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, ChinaThe Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital; The Second Affiliated Hospital of Guangzhou Medical University, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology; Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, ChinaSchool of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen 510006, ChinaNan Shan School, Guangzhou Medical University, Guangzhou 511436, ChinaSchool of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaGuangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510120, ChinaThe Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital; The Second Affiliated Hospital of Guangzhou Medical University, The State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology; Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, ChinaRecent studies have indicated that the Zika virus (ZIKV) has a significant impact on the fetal brain, and autophagy is contributing to host immune response and defense against virus infection. Here, we demonstrate that ZIKV infection triggered increased LC3 punctuation in mouse monocyte-macrophage cell line (RAW264.7), mouse microglial cell line (BV2), and hindbrain tissues, proving the occurrence of autophagy both in vitro and in vivo. Interestingly, manual intervention of autophagy, like deficiency inhibited by 3-MA, can reduce viral clearance in RAW264.7 cells upon ZIKV infection. Besides, specific siRNA strategy confirmed that autophagy can be activated through Atg7-Atg5 and type I IFN signaling pathway upon ZIKV infection, while knocking down of Atg7 and Atg5 effectively decreased the ZIKV clearance in phagocytes. Furthermore, we analyzed that type I IFN signaling could contribute to autophagic clearance of invaded ZIKV in phagocytes. Taken together, our findings demonstrate that ZIKV-induced autophagy is favorable to activate host immunity, particularly through type I IFN signaling, which participates in host protection and defense against ZIKV infection.http://dx.doi.org/10.1155/2020/9527147 |
| spellingShingle | Yuyi Huang Yujie Wang Shuhui Meng Zhuohang Chen Haifan Kong Ting Pan Gen Lu Xuefeng Li Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling Mediators of Inflammation |
| title | Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling |
| title_full | Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling |
| title_fullStr | Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling |
| title_full_unstemmed | Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling |
| title_short | Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling |
| title_sort | autophagy contributes to host immunity and protection against zika virus infection via type i ifn signaling |
| url | http://dx.doi.org/10.1155/2020/9527147 |
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