Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipation

The origin, development and differentiation of enteric nervous system neuroglia and its involvement in the pathogenesis of gastrointestinal diseases and neurodegenerative diseases have been little studied.Aim of this work is a comparative morphological study of glial cells in the ganglionic plexuses...

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Main Authors: E. I. Chumasov, N. A. Maistrenko, P. N. Romashchenko, V. B. Samedov, E. S. Petrova, D. E. Korzhevskii
Format: Article
Language:Russian
Published: Russian Academy of Sciences, Siberian Branch Publishing House 2024-01-01
Series:Сибирский научный медицинский журнал
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Online Access:https://sibmed.elpub.ru/jour/article/view/1326
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author E. I. Chumasov
N. A. Maistrenko
P. N. Romashchenko
V. B. Samedov
E. S. Petrova
D. E. Korzhevskii
author_facet E. I. Chumasov
N. A. Maistrenko
P. N. Romashchenko
V. B. Samedov
E. S. Petrova
D. E. Korzhevskii
author_sort E. I. Chumasov
collection DOAJ
description The origin, development and differentiation of enteric nervous system neuroglia and its involvement in the pathogenesis of gastrointestinal diseases and neurodegenerative diseases have been little studied.Aim of this work is a comparative morphological study of glial cells in the ganglionic plexuses of the enteric nervous system and analysis of neuroglial relationships in chronic slow-transit constipation using immunohistochemical methods.Material and methods. Resection material obtained at the Department of Faculty Surgery, S.P. Fedorov Faculty of Surgery of S.M. Kirov Military Medical Academy during planned surgical operations was used. The objects of the study were fragments of the sigmoid and colon obtained as a result of surgery for chronic slow-transit constipation (five cases, women aged 37–40 years). The study was carried out using immunohistochemical glial markers (GFAP, S100β protein, etc.).Results. Two types of glia were found in the myenteric ganglionic plexus of the large intestine: astrocyte-like and neurolemmocytic. The astrocyte-like type is similar to the neuroglia of the central nervous system, the neurolemmocytic type is similar to the glia of the autonomic nervous system. It has been established that astrocyte-like glia is found only in the Aauerbach ganglionic plexus, while neurolemmocytes are found in all innervated tissues of the intestinal wall. Reactive, dystrophic and degenerative changes in neurocytes, glial elements, agangliogenosis in the Auerbach plexus were found in all cases of chronic slow-transit constipation. Destructive changes in the neuromuscular terminal plexuses, interstitial edema and inflammatory monocytic reaction and leukocyte infiltration in the intestinal mucosa and intestinal submucosa, found in several cases.Conclusions. The results obtained allow classifying chronic slow-transit constipation as a neurodegenerative disease.
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spelling doaj-art-9a1628ecf2154f6bb9fc353132420d192025-08-20T03:50:44ZrusRussian Academy of Sciences, Siberian Branch Publishing HouseСибирский научный медицинский журнал2410-25122410-25202024-01-0143619120210.18699/SSMJ20230624576Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipationE. I. Chumasov0N. A. Maistrenko1P. N. Romashchenko2V. B. Samedov3E. S. Petrova4D. E. Korzhevskii5Institute of Experimental Medicine; Saint-Petersburg State University of Veterinary MedicineS.M. Kirov Military Medical AcademyS.M. Kirov Military Medical AcademyS.M. Kirov Military Medical AcademyInstitute of Experimental MedicineInstitute of Experimental MedicineThe origin, development and differentiation of enteric nervous system neuroglia and its involvement in the pathogenesis of gastrointestinal diseases and neurodegenerative diseases have been little studied.Aim of this work is a comparative morphological study of glial cells in the ganglionic plexuses of the enteric nervous system and analysis of neuroglial relationships in chronic slow-transit constipation using immunohistochemical methods.Material and methods. Resection material obtained at the Department of Faculty Surgery, S.P. Fedorov Faculty of Surgery of S.M. Kirov Military Medical Academy during planned surgical operations was used. The objects of the study were fragments of the sigmoid and colon obtained as a result of surgery for chronic slow-transit constipation (five cases, women aged 37–40 years). The study was carried out using immunohistochemical glial markers (GFAP, S100β protein, etc.).Results. Two types of glia were found in the myenteric ganglionic plexus of the large intestine: astrocyte-like and neurolemmocytic. The astrocyte-like type is similar to the neuroglia of the central nervous system, the neurolemmocytic type is similar to the glia of the autonomic nervous system. It has been established that astrocyte-like glia is found only in the Aauerbach ganglionic plexus, while neurolemmocytes are found in all innervated tissues of the intestinal wall. Reactive, dystrophic and degenerative changes in neurocytes, glial elements, agangliogenosis in the Auerbach plexus were found in all cases of chronic slow-transit constipation. Destructive changes in the neuromuscular terminal plexuses, interstitial edema and inflammatory monocytic reaction and leukocyte infiltration in the intestinal mucosa and intestinal submucosa, found in several cases.Conclusions. The results obtained allow classifying chronic slow-transit constipation as a neurodegenerative disease.https://sibmed.elpub.ru/jour/article/view/1326human colonsigmoid colonchronic slow-transit constipationglias100β proteingfapimmunohistochemistry
spellingShingle E. I. Chumasov
N. A. Maistrenko
P. N. Romashchenko
V. B. Samedov
E. S. Petrova
D. E. Korzhevskii
Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipation
Сибирский научный медицинский журнал
human colon
sigmoid colon
chronic slow-transit constipation
glia
s100β protein
gfap
immunohistochemistry
title Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipation
title_full Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipation
title_fullStr Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipation
title_full_unstemmed Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipation
title_short Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipation
title_sort pathological changes of glial cells in the enteric nervous system of the colon with chronic slow transit constipation
topic human colon
sigmoid colon
chronic slow-transit constipation
glia
s100β protein
gfap
immunohistochemistry
url https://sibmed.elpub.ru/jour/article/view/1326
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