The distribution of four homeobox proteins in the bovine stomach compartments during the fetal period

Homeobox proteins undertake important roles in the determination of the cell identity during embryonic development, the differentiation of embryonic stem cells, morphogenesis, and the formation and development of the mammalian gastrointestinal tract. Based on these data, this study was designed to...

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Main Authors: Uğur Topaloğlu, Mehmet Erdem-Akbalık, Hakan Sağsöz, Muzaffer Aydın-Ketani, Berna Güney-Saruhan, Nurşin Aydın
Format: Article
Language:English
Published: Universidad del Zulia 2025-04-01
Series:Revista Científica
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Online Access:https://produccioncientificaluz.org/index.php/cientifica/article/view/43747
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author Uğur Topaloğlu
Mehmet Erdem-Akbalık
Hakan Sağsöz
Muzaffer Aydın-Ketani
Berna Güney-Saruhan
Nurşin Aydın
author_facet Uğur Topaloğlu
Mehmet Erdem-Akbalık
Hakan Sağsöz
Muzaffer Aydın-Ketani
Berna Güney-Saruhan
Nurşin Aydın
author_sort Uğur Topaloğlu
collection DOAJ
description Homeobox proteins undertake important roles in the determination of the cell identity during embryonic development, the differentiation of embryonic stem cells, morphogenesis, and the formation and development of the mammalian gastrointestinal tract. Based on these data, this study was designed to determine the immunohistochemical localization and expression levels of HOXA10, HOXA11, HOXC6 and HOXB6, which are subunits of the homeobox proteins, in the rumen, reticulum, omasum and abomasum during fetal development. For this purpose, fetuses obtained from private slaughterhouses and were grouped according to their crown-rump length (CRL) measurements, and the gestational trimester they belonged to, as follows: first trimester (69-89 days old/10 fetuses), second trimester (99-178 days old/10 fetuses) and third trimester (188-269 days old/10 fetuses). Gastric tissue samples taken from each group underwent routine histological processing and immunohistochemical staining. Immunohistochemical staining demonstrated that the HOXA10, HOXA11 and HOXC6 proteins were expressed at varying levels in the rumen, reticulum, omasum and abomasum, and their expression was stronger in the epithelial and smooth muscle cells. On the other hand, while there was almost no expression of HOXB6 in the epithelial cells of the rumen during the second and third trimesters of gestation, the remaining gastric components were immunonegative. Based on these findings, it was concluded that some homeobox proteins could have critical roles in the development, morphogenesis and histogenesis of fetal bovine gastric compartments, and thus, could contribute to the lifetime performance and productivity of cattle in terms of milk and meat yields.
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spelling doaj-art-9a0f53b66f6d4736afcb8bb053b60aee2025-08-20T02:25:18ZengUniversidad del ZuliaRevista Científica0798-22592521-97152025-04-0135210.52973/rcfcv-e35586The distribution of four homeobox proteins in the bovine stomach compartments during the fetal periodUğur Topaloğlu0Mehmet Erdem-Akbalık1Hakan Sağsöz2Muzaffer Aydın-Ketani3Berna Güney-Saruhan4Nurşin Aydın5Department of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, 21280, Diyarbakır, Türkiye.Department of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, 21280, Diyarbakır, Türkiye.Department of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, 21280, Diyarbakır, Türkiye.Department of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, 21280, Diyarbakır, Türkiye.Department of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, 21280, Diyarbakır, Türkiye.Department of Histology and Embryology, Faculty of Veterinary Medicine, Dicle University, 21280, Diyarbakır, Türkiye. Homeobox proteins undertake important roles in the determination of the cell identity during embryonic development, the differentiation of embryonic stem cells, morphogenesis, and the formation and development of the mammalian gastrointestinal tract. Based on these data, this study was designed to determine the immunohistochemical localization and expression levels of HOXA10, HOXA11, HOXC6 and HOXB6, which are subunits of the homeobox proteins, in the rumen, reticulum, omasum and abomasum during fetal development. For this purpose, fetuses obtained from private slaughterhouses and were grouped according to their crown-rump length (CRL) measurements, and the gestational trimester they belonged to, as follows: first trimester (69-89 days old/10 fetuses), second trimester (99-178 days old/10 fetuses) and third trimester (188-269 days old/10 fetuses). Gastric tissue samples taken from each group underwent routine histological processing and immunohistochemical staining. Immunohistochemical staining demonstrated that the HOXA10, HOXA11 and HOXC6 proteins were expressed at varying levels in the rumen, reticulum, omasum and abomasum, and their expression was stronger in the epithelial and smooth muscle cells. On the other hand, while there was almost no expression of HOXB6 in the epithelial cells of the rumen during the second and third trimesters of gestation, the remaining gastric components were immunonegative. Based on these findings, it was concluded that some homeobox proteins could have critical roles in the development, morphogenesis and histogenesis of fetal bovine gastric compartments, and thus, could contribute to the lifetime performance and productivity of cattle in terms of milk and meat yields. https://produccioncientificaluz.org/index.php/cientifica/article/view/43747bovinefetushomeobox proteinsstomach
spellingShingle Uğur Topaloğlu
Mehmet Erdem-Akbalık
Hakan Sağsöz
Muzaffer Aydın-Ketani
Berna Güney-Saruhan
Nurşin Aydın
The distribution of four homeobox proteins in the bovine stomach compartments during the fetal period
Revista Científica
bovine
fetus
homeobox proteins
stomach
title The distribution of four homeobox proteins in the bovine stomach compartments during the fetal period
title_full The distribution of four homeobox proteins in the bovine stomach compartments during the fetal period
title_fullStr The distribution of four homeobox proteins in the bovine stomach compartments during the fetal period
title_full_unstemmed The distribution of four homeobox proteins in the bovine stomach compartments during the fetal period
title_short The distribution of four homeobox proteins in the bovine stomach compartments during the fetal period
title_sort distribution of four homeobox proteins in the bovine stomach compartments during the fetal period
topic bovine
fetus
homeobox proteins
stomach
url https://produccioncientificaluz.org/index.php/cientifica/article/view/43747
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