CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering
Abstract Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types w...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2020-06-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.20209475 |
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| _version_ | 1850201901892632576 |
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| author | Daniel Paolo Anderson Henry James Benns Edward William Tate Matthew Andrew Child |
| author_facet | Daniel Paolo Anderson Henry James Benns Edward William Tate Matthew Andrew Child |
| author_sort | Daniel Paolo Anderson |
| collection | DOAJ |
| description | Abstract Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology‐directed repair for engineering purposes, removing time‐consuming post hoc curation, simplifying gRNA outputs and reducing CPU times. |
| format | Article |
| id | doaj-art-99f8136a3dd34f8bb685d5a240a61272 |
| institution | OA Journals |
| issn | 1744-4292 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-99f8136a3dd34f8bb685d5a240a612722025-08-20T02:11:54ZengSpringer NatureMolecular Systems Biology1744-42922020-06-011661710.15252/msb.20209475CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineeringDaniel Paolo Anderson0Henry James Benns1Edward William Tate2Matthew Andrew Child3Department of Life Sciences, Imperial College LondonDepartment of Life Sciences, Imperial College LondonDepartment of Chemistry, Imperial College LondonDepartment of Life Sciences, Imperial College LondonAbstract Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology‐directed repair for engineering purposes, removing time‐consuming post hoc curation, simplifying gRNA outputs and reducing CPU times.https://doi.org/10.15252/msb.20209475CRISPRgenome engineeringprotein‐centrictarget prioritization |
| spellingShingle | Daniel Paolo Anderson Henry James Benns Edward William Tate Matthew Andrew Child CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering Molecular Systems Biology CRISPR genome engineering protein‐centric target prioritization |
| title | CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering |
| title_full | CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering |
| title_fullStr | CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering |
| title_full_unstemmed | CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering |
| title_short | CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering |
| title_sort | crispr tape protein centric crispr guide design for targeted proteome engineering |
| topic | CRISPR genome engineering protein‐centric target prioritization |
| url | https://doi.org/10.15252/msb.20209475 |
| work_keys_str_mv | AT danielpaoloanderson crisprtapeproteincentriccrisprguidedesignfortargetedproteomeengineering AT henryjamesbenns crisprtapeproteincentriccrisprguidedesignfortargetedproteomeengineering AT edwardwilliamtate crisprtapeproteincentriccrisprguidedesignfortargetedproteomeengineering AT matthewandrewchild crisprtapeproteincentriccrisprguidedesignfortargetedproteomeengineering |