CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering
Abstract Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types w...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2020-06-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.15252/msb.20209475 |
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| Summary: | Abstract Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology‐directed repair for engineering purposes, removing time‐consuming post hoc curation, simplifying gRNA outputs and reducing CPU times. |
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| ISSN: | 1744-4292 |