Promoting Effects of <i>Piriformospora indica</i> on Plant Growth and Development of Tissue-Cultured <i>Cerasus humilis</i> Seedlings

<i>Piriformospora indica</i> is a beneficial endophytic fungus that promotes plant growth and root development by colonizing plant roots. In order to investigate whether <i>P. indica</i> could promote the growth of tissue-cultured <i>Cerasus humilis</i> seedlings,...

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Main Authors: Lu Yin, JinYang Cheng, YunPeng Liu, YinTao Guan, LuTing Jia, Shuai Zhang, PengFei Wang, XiaoPeng Mu, JianCheng Zhang
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/11/7/797
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Summary:<i>Piriformospora indica</i> is a beneficial endophytic fungus that promotes plant growth and root development by colonizing plant roots. In order to investigate whether <i>P. indica</i> could promote the growth of tissue-cultured <i>Cerasus humilis</i> seedlings, in this study, we co-cultivated <i>P. indica</i> colony segments (P+) and <i>P. indica</i> spore suspensions (P++) in the rooting medium, and plant biomass as well as chlorophyll and root hormone contents of ‘3-19-3’ tissue-cultured <i>C. humilis</i> seedlings were determined under P+, P++, and CK (without fungus inoculation) treatments. The results showed that above-ground biomass and chlorophyll content of P+-and P++-treated tissue-cultured seedlings were significantly increased, and root peroxidase (POD), indole-3-acetic-acid (IAA) content, and root activities were significantly enhanced, while jasmonic acid (JA) and 1-aminocyclopropane-1-carboxylic acid (ACC) contents were reduced. Moreover, the growth-promoting effects of P++ treatment were found to be stronger than those of P+ treatment. Our results confirmed that <i>P. indica</i> was able to promote the growth of tissue-cultured <i>C. humilis</i> seedlings and effectively promoted root development by regulating hormone content. Therefore, the application of <i>P. indica</i> in the production of <i>C. humilis</i> is promising, especially in the cultivation of elite varieties.
ISSN:2311-7524